Thrombin, Pig blood
Based on 8 publication(s) in Google Scholar
Rabbit Thrombin is a Na+-activated, allosteric serine protease that plays opposing functional roles in blood coagulation. Thrombin recognition sequence and can be used to digest GST-tagged proteins.
For research use only. We do not sell to patients.
- Purity: 88.00%
- CAS No.: 9002-04-4
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Publications Citing Use of MedChemExpress (MCE) Thrombin, Pig blood
More- Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
- Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
- Stem Cell Res Ther. 2020 Feb 21;11(1):76. [Abstract]
- Front Pharmacol. 2022 Jan 10;12:792263. [Abstract]
- Antiviral Res. 2023 Jun:214:105606. [Abstract]
- Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
- University of Utah. 2026.
- bioRxiv. 2026 Mar 5:2026.03.03.709348. [Abstract]
Customer Validation & Images
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Flow Cytometry
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Cell Imaging/Staining
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Cell Migration/Invasion Assay
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WB
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ELISA
Biological Activity
Product information
This product is extracted from porcine plasma, with a protein content of ≥85% (biuret). It can hydrolyze fibrinogen and is used as a raw material for blood coagulation diagnostic reagents for detecting fibrinogen content, as well as a pharmaceutical raw material for treating gastrointestinal bleeding.
Instructions
Prepare a thrombin solution containing 100 U/mL with 0.1% (w/v) BSA solution. The enzyme activity of the stored solution can be retained for one week at 0-4°C.
Thrombin, Pig blood is a key enzyme in the coagulation cascade, responsible for converting fibrinogen into fibrin. Thrombin, Pig blood activates platelet activation, endothelial cell function, and inflammatory responses by binding to PAR-1 and PAR-4, which triggers G protein-coupled signaling pathways. Thrombin, Pig blood holds promise for research in coagulation and inflammation fields[2][3].
Thrombin, Pig blood exhibits a good affinity to thrombomodulin TM456 with a KD of 1.6 nM, which converts the procoagulant activity of Thrombin, Pig blood into anticoagulant and antiplasmin properties[4].
Thrombin, Pig blood induces PAR1-related intracellular Ca2+ release in cell MDA-MB 231 and A549 with EC50s of 2.57 nM and 0.53 nM[5].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
3.4.21.5
≥100 IU/mg soild
It was determined by comparison with the Thrombin reference standard
Chemical Information
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CAS No. 9002-04-4
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Appearance Solid
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Color White to off-white
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SMILES
[Thrombin, Pig blood]
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Cancer Commun (Lond)
Reciprocal tumor-platelet interaction through the EPHB1-EFNB1 axis in the liver metastatic niche promotes metastatic tumor outgrowth in pancreatic ductal adenocarcinoma. [Abstract]2025 Feb;45(2):143-166. PMID: 39648610
Thrombin, Pig blood purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Feb;45(2):143-166. [Abstract]
Platelets were treated with Fc fragment, Thrombin (1 µg/mL; 2 h), EphB1‐Fc, Thrombin + EphB1‐Fc, mono EphB1, mono EphB1 + EphB1‐Fc, or EFNB1 Ab + EphB1‐Fc. Platelets without treatment served as controls. CD62P expression in activated platelets was tested by flow cytometry analysis. Representative flow cytometry analysis of CD62P expression is presented on the left. The right graph shows the percentage of CD62P+ platelets. Data were presented as the means ± SD, with n = 3 per group.
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Allergy
2022 Jul;77(7):2104-2120. PMID: 34995358
Thrombin, Pig blood purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
Western blots for the HA tag on RRL-expressed IL-33 showing the Thrombin cleavage of human pro-IL-33 into the two main forms, ~18 and ~25 kDa, and the inhibition of these cleavage events by the Thrombin inhibitor Argatroban (50 μg/ml). Cleavage occurred in a time-dependent manner (15 min, 30 min, 1 h, 2 h and 4 h; 0.075 U Thrombin; 15 μL buffer) (A) and a dose-dependent manner (0.01875, 0.0375, 0.075, 0.15 and 0.3 U; RT for 1 h; 15 μL buffer) (B).
Thrombin, Pig blood purchased from MedChemExpress. Usage Cited in: Allergy. 2022 Jul;77(7):2104-2120. [Abstract]
L-33-dependent IL-5, IL-13 and IL-4 production by ST2+ CD4 cells incubated with or without Thrombin (50 pg RRL; 0.075 U Thrombin; RT for 1 h) and with or without a neutralizing hIL-33 mAb. The bars and error bars represent the mean ± SEM; *p < .05; **p < .01; ***p < .001.
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Stem Cell Res Ther
Effects of SW033291 on the myogenesis of muscle-derived stem cells and muscle regeneration. [Abstract]2020 Feb 21;11(1):76. PMID: 32085799 -
Front Pharmacol
Hsp47 Inhibitor Col003 Attenuates Collagen-Induced Platelet Activation and Cerebral Ischemic-Reperfusion Injury in Rats. [Abstract]2022 Jan 10;12:792263. PMID: 35082674 -
Antiviral Res
Omicsynin B4 potently blocks coronavirus infection by inhibiting host proteases cathepsin L and TMPRSS2. [Abstract]2023 Jun:214:105606. PMID: 37076089 -
Mol Carcinog
Baicalein promotes KDM4E to induce BICD1 and inhibit triple-negative breast cancer progression by blocking PAR1 signaling. [Abstract]2024 Jul;63(7):1288-1302. PMID: 38607237
Thrombin, Pig blood purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the proliferative activity of Thrombin (1 pM)-activated TNBC cells detected by EdU staining (scale bar: 50 μm).
Thrombin, Pig blood purchased from MedChemExpress. Usage Cited in: Mol Carcinog. 2024 Jul;63(7):1288-1302. [Abstract]
Effect of knockdown of KDM4E and combined overexpression of BICD1 on the invasive activity of Thrombin (1 pM)-activated TNBC cells detected by Transwell assay (scale bar: 50 μm). *p < 0.05 versus the sh-scramble group, #p < 0.05 versus the sh-KDM4E + oe-NC group, &p < 0.05 versus the blank group.
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bioRxiv
Protease-Activated Receptor 1 as an Endogenous Model of Peptidergic Gαq-Gα12-Biased G Protein Signaling. [Abstract]2026 Mar 5:2026.03.03.709348. PMID: 41847045
Solvent & Solubility
H2O : ≥ 100 mg/mL
* "≥" means soluble, but saturation unknown.
Purity & Documentation
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Data Sheet (268 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Golderman V, et al. Thrombin and the Protease-Activated Receptor-1 in Organophosphate-Induced Status Epilepticus. J Mol Neurosci. 2018 Dec 4. [Content Brief]
[2]. Coughlin SR. Thrombin signalling and protease-activated receptors. Nature. 2000 Sep 14;407(6801):258-64. [Content Brief]
[3]. Lundblad RL, et al. A review of the therapeutic uses of thrombin. Thromb Haemost. 2004 May;91(5):851-60. [Content Brief]
[4]. Chen K, et al., Exosite 2-Directed Ligands Attenuate Protein C Activation by the Thrombin-Thrombomodulin Complex. Biochemistry. 2017 Jun 20;56(24):3119-3128. [Content Brief]
[5]. Stahn S, et al., Teleocidin A2 inhibits human proteinase-activated receptor 2 signaling in tumor cells. Pharmacol Res Perspect. 2016 Jun 10;4(4):e00230. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Thrombin, Pig blood Related Classifications
HY-114164C Related Classifications
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