Lucifer Yellow CH dilithium salt

Name: Lucifer Yellow CH dilithium salt
Brand: MedChemExpress
SKU: HY-128692
Rating: 4.5 (1 reviews)

Cat. No.: HY-128692 Purity: ≥98.0%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

Lucifer Yellow CH dilithium salt is a high-intensity fluorescent probe containing free hydrazyl groups. Lucifer Yellow CH can react with fatty aldehydes at room temperature. Lucifer Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Lucifer yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.

For research use only. We do not sell to patients.

Lucifer Yellow CH dilithium salt Chemical Structure

CAS No. : 67769-47-5

Size	Price	Stock	Quantity
5 mg	USD 90	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 145	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Lucifer Yellow CH dilithium salt:

Lucifer Yellow CH dipotassium salt In-stock

1 Publications Citing Use of MCE Lucifer Yellow CH dilithium salt

•Free Radic Biol Med. 2021 Dec 6;178:271-294. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Preparation of Lucifer Yellow CH (dilithium salt) working solution
1 Preparation of the stock solution
Dissolve Lucifer Yellow CH (dilithium salt) in DDH₂O to obtain 1 mg/mL of Lucifer Yellow CH (dilithium salt).
Note: It is recommended to store the stock solution at -20°C -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Preparation of Lucifer Yellow CH (dilithium salt) working solution.
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 0.5% of Lucifer Yellow CH (dilithium salt) working solution.
Note: Please adjust the concentration of Lucifer Yellow CH (dilithium salt) working solution according to the actual situation.

Cell staining
1. Cell preparation:
For suspension cells: Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
For adherent cells: Discard the cell culture medium, and add trypsin to dissociate cells to make a single-cell suspension. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time.
2. Add 1 mL of Lucifer Yellow CH (dilithium salt) working solution, and then incubate at room temperature for 5-30 minutes.
3. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
4. Wash twice with PBS, 5 minutes each time.
5. Resuspend cells with serum-free cell culture medium or PBS, and then detect by fluorescence microscope or flow cytometer.

Precautions
1. It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
2. Please adjust the concentration of Lucifer Yellow CH (dilithium salt) working solution according to the actual situation.
3. This product is for R&D use only, not for drug, household, or other uses.
4. For your safety and health, please wear a lab coat and disposable gloves to operate.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

457.25

Formula

C₁₃H₉Li₂N₅O₉S₂

CAS No.

67769-47-5

Appearance

Solid

Color

Yellow to orange

Emission (Em)

536

Excitation (Ex)

428

SMILES

O=S(C1=C(N)C2=CC(S(=O)([O-])=O)=CC(C(N(NC(NN)=O)C3=O)=O)=C2C3=C1)([O-])=O.[Li+].[Li+]

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (54.67 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1870 mL	10.9349 mL	21.8699 mL
5 mM	0.4374 mL	2.1870 mL	4.3740 mL
10 mM	0.2187 mL	1.0935 mL	2.1870 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo:

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: PBS
Solubility: 10 mg/mL (21.87 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
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Dosing volume
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μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.04%

Select Batch:

Data Sheet (280 KB) SDS (0 KB)

COA (257 KB) HNMR (275 KB) LCMS (94 KB)

Handling Instructions (2659 KB)

References

[1]. Stewart WW. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 1978 Jul;14(3):741-59. [Content Brief]

[2]. Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.1870 mL	10.9349 mL	21.8699 mL	54.6747 mL
	5 mM	0.4374 mL	2.1870 mL	4.3740 mL	10.9349 mL
	10 mM	0.2187 mL	1.0935 mL	2.1870 mL	5.4675 mL
	15 mM	0.1458 mL	0.7290 mL	1.4580 mL	3.6450 mL
	20 mM	0.1093 mL	0.5467 mL	1.0935 mL	2.7337 mL
	25 mM	0.0875 mL	0.4374 mL	0.8748 mL	2.1870 mL
	30 mM	0.0729 mL	0.3645 mL	0.7290 mL	1.8225 mL
	40 mM	0.0547 mL	0.2734 mL	0.5467 mL	1.3669 mL
	50 mM	0.0437 mL	0.2187 mL	0.4374 mL	1.0935 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.