Luc Yellow CH dilithium

Name: Luc Yellow CH dilithium
Brand: MedChemExpress
SKU: HY-128692
Rating: 5.0 (5 reviews)

Cat. No.: HY-128692 Purity: 99.67%: Data Sheet
COA

SDS
Handling Instructions
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Luc Yellow CH dilithium is a high-intensity fluorescent probe containing free hydrazyl groups. Luc Yellow CH can react with fatty aldehydes at room temperature. Luc Yellow CH serves as a biological tracer to monitor neuronal branching, regeneration, gap junction detection and characterization, and selective ablation of cells after aldehyde fixation. Luc Yellow CH displays the maximum excitation/emission of 430 nm/540 nm, respectively.

For research use only. We do not sell to patients.

CAS No. : 67769-47-5

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in Water ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in Water	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 5 publication(s) in Google Scholar

Other Forms of Luc Yellow CH dilithium:

Lucifer Yellow CH dipotassium salt In-stock

5 Publications Citing Use of MCE Luc Yellow CH dilithium

Others

Cell Imaging/Staining

: Luc Yellow CH dilithium purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2025 Mar 18:32:101677.; Lucifer yellow CH (10 μg/mL; 12 h) staining images of DPP, GLUT-PP and POSS-PEG-PP.

: Luc Yellow CH dilithium purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Oct 6;15(1):34716. [Abstract]; The H9c2 cells were treated in normoxia and hypoxia for the indicated times, and the distance of LY dye (Luc Yellow CH dilithium: 1mg/mL; 5 min) spread was measured to evaluate the GJ functionality.

: Luc Yellow CH dilithium purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Oct 6;15(1):34716. [Abstract]; The H/R-treated H9c2 cells were cultured in a culture medium containing SP15 (50 µM) for 24 h, and the distance of LY dye (Luc Yellow CH dilithium: 1mg/mL; 5 min) spread was measured to evaluate the GJ functionality.

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1 Preparation of dye working solution ^[3]
1) Prepare 0.33 M lithium citrate solution containing 5% Lucifer Yellow CH as the working solution.
2 Specific staining steps
1) Insert a glass capillary microelectrode containing Lucifer Yellow CH working solution into the neuron and apply a constant hyperpolarizing current (0.2-0.5 nA) for 5-15 min for ion electrophoresis injection.
2) Simultaneously monitor the DC potential and action potential induced by intracellular stimulation. Only one cell is labeled per slice.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

457.25

Formula

C₁₃H₉Li₂N₅O₉S₂

CAS No.

67769-47-5

Appearance

Solid

Color

Yellow to orange

Emission (Em)

536

Excitation (Ex)

428

SMILES

O=S(C1=C(N)C2=CC(S(=O)(O[Li])=O)=CC(C(N(NC(NN)=O)C3=O)=O)=C2C3=C1)(O[Li])=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 25 mg/mL (54.67 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1870 mL	10.9349 mL	21.8699 mL
5 mM	0.4374 mL	2.1870 mL	4.3740 mL
10 mM	0.2187 mL	1.0935 mL	2.1870 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

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(per animal)

μL

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.67%

Select Batch:

Data Sheet (283 KB) SDS (252 KB)

COA (257 KB) HNMR (271 KB) RP-HPLC (278 KB) LCMS (93 KB)

Handling Instructions (2659 KB)

References

[1]. Stewart WW. Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell. 1978 Jul;14(3):741-59. [Content Brief]

[2]. Klein M, et al. Transport of lucifer yellow CH into plant vacuoles--evidence for direct energization of a sulphonatedsubstance and implications for the design of new molecular probes. FEBS Lett. 1997 Dec 22;420(1):86-92. [Content Brief]

[3]. Kawata M, et al. Immunohistochemical identification of Lucifer Yellow-labeled neurons in the rat supraoptic nucleus. Histochemistry. 1983;78(1):21-6. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O	1 mM	2.1870 mL	10.9349 mL	21.8699 mL	54.6747 mL
	5 mM	0.4374 mL	2.1870 mL	4.3740 mL	10.9349 mL
	10 mM	0.2187 mL	1.0935 mL	2.1870 mL	5.4675 mL
	15 mM	0.1458 mL	0.7290 mL	1.4580 mL	3.6450 mL
	20 mM	0.1093 mL	0.5467 mL	1.0935 mL	2.7337 mL
	25 mM	0.0875 mL	0.4374 mL	0.8748 mL	2.1870 mL
	30 mM	0.0729 mL	0.3645 mL	0.7290 mL	1.8225 mL
	40 mM	0.0547 mL	0.2734 mL	0.5467 mL	1.3669 mL
	50 mM	0.0437 mL	0.2187 mL	0.4374 mL	1.0935 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.