Hydroxystilbamidine bis(fluoroGlod)

Name: Hydroxystilbamidine bis(fluoroGlod)
Brand: MedChemExpress
SKU: HY-108166A
Rating: 4.5 (1 reviews)

Cat. No.: HY-108166A Purity: ≥96.0%: FAQs
Data Sheet SDS COA Handling Instructions

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Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.

For research use only. We do not sell to patients.

Hydroxystilbamidine bis(fluoroGlod) Chemical Structure

CAS No. : 223769-64-0

Size	Price	Stock	Quantity
1 mg	USD 85	In-stock	Estimated Time of Arrival: December 31
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10 mg		Get quote

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Based on 1 publication(s) in Google Scholar

Other Forms of Hydroxystilbamidine bis(fluoroGlod):

Hydroxystilbamidine Get quote

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Biological Activity
Protocol
Purity & Documentation
References
Customer Review

Description

Hydroxystilbamidine bis(methanesulfonate), a dye capable of binding to both DNA and RNA, has been found to be a powerful inhibitor of cellular ribonucleases.

In Vitro

It is found that the trypanocidal dye Hydroxystilbamidine bis(methanesulfonate) permits the recovery of mRNA after polysome released with Nonidet P-40 (NP-40). Sucrose gradient analysis of detergent-lysed postnuclear supernates is used to analyze the size distribution of NP-40-released polysomes. The heparin gradient shows some polyribosomes, whereas the Hydroxystilbamidine bis(methanesulfonate) gradient shows a remarkably large peak of very heavy polyribosomes. This peak is obtained reproducibly if Hydroxystilbamidine bis(methanesulfonate) is present before the addition of NP-40^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Hydroxystilbamidine bis(methanesulfonate) is an effective suppressor of the plaque-forming cell (PFC) response when given before sheep erythrocytes (SRBC) stimulation. Hydroxystilbamidine bis(methanesulfonate) depresses the plaque response of the treated mice. Fewer PFC are observed in Hydroxystilbamidine bis(methanesulfonate)-treated mice throughout the experiment, but the level of suppression decreases with time. By day 14, the number of PFC observed in both the Hydroxystilbamidine bis(methanesulfonate) treated mice and the control group is essentially at the background level^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

472.54

Formula

C₁₈H₂₄N₄O₇S₂

CAS No.

223769-64-0

Appearance

Solid

Color

Light yellow to yellow

Emission (Em)

625

Excitation (Ex)

360

SMILES

NC(C1=CC=C(/C=C/C2=C(O)C=C(C(N)=N)C=C2)C=C1)=N.OS(=O)(C)=O.OS(=O)(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

H₂O : 50 mg/mL (105.81 mM; ultrasonic and warming and heat to 60°C)

DMSO : 50 mg/mL (105.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.1162 mL	10.5811 mL	21.1622 mL
5 mM	0.4232 mL	2.1162 mL	4.2324 mL
10 mM	0.2116 mL	1.0581 mL	2.1162 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: ≥96.0%

Select Batch:

Data Sheet (277 KB) SDS (393 KB)

COA (251 KB)

Handling Instructions (2659 KB)

References

[1]. Lizardi PM. Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine. J Cell Biol. 1980 Oct;87(1):292-6. [Content Brief]

[2]. Folds JD, et al. Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug. Infect Immun. 1975 Mar;11(3):441-4. [Content Brief]

Kinase Assay ^[1]	Silkwork larvae are used in this study. Larvae on the fourth or fifth day of the fifth instar (~4.2 g body weight) are injected with 35 μL of a solution of 10 mg/mL cyclobeximidein H₂O. After 5 min, the animals are immobilized in ice, and posterior silk glands are dissected and washed in ice-cold 0.15 M NaCl, 0.015 M Na citrate, 100 μg/mL cycloheximide. Washed glands from two larvae are placed in a homogenizer containing 4.7 mL of 40 mM triethanolamine-HCl, pH 7.5, 0.15 M sucrose, 0.1 M KCI, 3 mM MgCl₂, 2 mM reduced glutathione, 10 μg/mL cycloheximide, 750 μg/mL Escherichia coli tRNA, and an appropriate concentration of RNase inhibitor (sodium heparin, 1.5 μg/mL or Hydroxystilbamidine bis(methanesulfonate), 1.5 mM)^[1]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
Animal Administration ^[2]	Mice (six per group) are given various doses of Hydroxystilbamidine bis(methanesulfonate) (HSB) 3, 2, and 1 day before antigen. Other groups are given Hydroxystilbamidine bis(methanesulfonate) 1 or 2 days after the injection of antigen. Another group of mice receive antigen and Hydroxystilbamidine bis(methanesulfonate) simultaneously. A control group receives only antigen. The antigen dose consists of 2×10⁸ sheep erythrocytes (SRBC). Four days after the injection of SRBC, the mice are sacrificed and spleens are removed and assayed for plaque-forming cell (PFC) by the plaque assay^[2]. MCE has not independently confirmed the accuracy of these methods. They are for reference only.
References	[1]. Lizardi PM. Isolation of giant silk fibroin polysomes and fibroin mRNP particles using a novel ribonuclease inhibitor, hydroxystilbamidine. J Cell Biol. 1980 Oct;87(1):292-6. [Content Brief] [2]. Folds JD, et al. Immunosuppression by hydroxystilbamidine isethionate, a lysosome-stabilizing, anti-proteolytic, antifungal drug. Infect Immun. 1975 Mar;11(3):441-4. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	2.1162 mL	10.5811 mL	21.1622 mL	52.9056 mL
	5 mM	0.4232 mL	2.1162 mL	4.2324 mL	10.5811 mL
	10 mM	0.2116 mL	1.0581 mL	2.1162 mL	5.2906 mL
	15 mM	0.1411 mL	0.7054 mL	1.4108 mL	3.5270 mL
	20 mM	0.1058 mL	0.5291 mL	1.0581 mL	2.6453 mL
	25 mM	0.0846 mL	0.4232 mL	0.8465 mL	2.1162 mL
	30 mM	0.0705 mL	0.3527 mL	0.7054 mL	1.7635 mL
	40 mM	0.0529 mL	0.2645 mL	0.5291 mL	1.3226 mL
	50 mM	0.0423 mL	0.2116 mL	0.4232 mL	1.0581 mL
	60 mM	0.0353 mL	0.1764 mL	0.3527 mL	0.8818 mL
	80 mM	0.0265 mL	0.1323 mL	0.2645 mL	0.6613 mL
	100 mM	0.0212 mL	0.1058 mL	0.2116 mL	0.5291 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.