1. Membrane Transporter/Ion Channel
    Neuronal Signaling
  2. TRP Channel
    Sodium Channel
  3. EIPA

EIPA (Synonyms: L593754; MH 12-43)

Cat. No.: HY-101840 Purity: 99.73%
Handling Instructions

EIPA (L593754) est un inhibiteur de canal TRPP3 avec un IC50 de 10,5 μM. EIPA inhibe également les échangeurs Na+/H+ (NHE) et macropinocytose.

EIPA (L593754) is a TRPP3 channel inhibitor with an IC50 of 10.5 μM. EIPA also inhibits Na+/H+-exchanger (NHE) and macropinocytosis.

For research use only. We do not sell to patients.

EIPA Chemical Structure

EIPA Chemical Structure

CAS No. : 1154-25-2

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Based on 3 publication(s) in Google Scholar

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Description

EIPA (L593754) is a TRPP3 channel inhibitor with an IC50 of 10.5 μM. EIPA also inhibits Na+/H+-exchanger (NHE) and macropinocytosis[1][2][3].

IC50 & Target

IC50: 10.5 μM (TRPP3 channel)[1]
NHE[2]
Macropinocytosis[3]

In Vitro

In the presence of 100 μM EIPA, 10 μM benzamil, and 10 μM phenamil, 45Ca2+ uptake decreases from 79±9 to 46±4 (58% remaining), 27±4 (34%), 29±5 (37%), and 38±4 (48%) pmol/oocyte/30 min (n=6, P=0.008), respectively. It is found that EIPA, benzamil, and phenamil rapidly and reversibly block Ca2+-activated TRPP3 channel activation at -50 mV, with IC50s of 143±8 (n=36), 10.5±2.2 (n=28), 1.1±0.3 (n=30), and 0.14±0.04 μM (n=25), respectively[1]. The number of autophagic vacuoles increases dramatically in the HAE and HPE groups after EIPA treatment compare with the HAN and HPN groups. EIPA regulates the initiation and maturation of the autophagy associated with amino acids in IEC-18 cells[2]. In addition, the uptake of cinnamoylphenazine (CA-PZ) and neutral red (NR) is inhibited by EIPA[3].

Molecular Weight

299.76

Formula

C₁₁H₁₈ClN₇O

CAS No.

1154-25-2

SMILES

O=C(C1=NC(Cl)=C(N(CC)C(C)C)N=C1N)NC(N)=N

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
  -20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 140 mg/mL (467.04 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.3360 mL 16.6800 mL 33.3600 mL
5 mM 0.6672 mL 3.3360 mL 6.6720 mL
10 mM 0.3336 mL 1.6680 mL 3.3360 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.33 mg/mL (7.77 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.33 mg/mL (7.77 mM); Clear solution

*All of the co-solvents are provided by MCE.
References
Cell Assay
[2]

The effect of EIPA alone (without alanine or proline) is also examined in both control (DMEM cultured cells) and amino acid-starved cells. The cells are incubated for 6 h in DMEM containing 5% FBS and either 0 or 0.3 mM EIPA (labelled QNN and QNE, respectively), HBSS containing either 0 or 0.3 mM EIPA (labelled HNN and HNE, respectively), HBSS with 1.0 mM alanine (labelled HAN) or 0.5 mM proline (labelled HPN), HBSS with 1.0 mM alanine and 0.3 mM EIPA (labelled HAE), and HBSS with 0.5 mM proline and 0.3 mM EIPA (labelled HPE)[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: 99.73%

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Keywords:

EIPAL593754MH 12-43L 593754L-593754TRP ChannelSodium ChannelTransient receptor potential channelsNa channelsNa+ channelsInhibitorinhibitorinhibit

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