2. Membrane Transporter/Ion Channel
    Neuronal Signaling
    Autophagy
    Immunology/Inflammation
    GPCR/G Protein
  3. TRP Channel
    Autophagy
    COX
    Prostaglandin Receptor
    Na+/H+ Exchanger (NHE)
  4. EIPA hydrochloride

EIPA hydrochloride  (Synonyms: L593754 hydrochloride; MH 12-43 hydrochloride; Ethylisopropylamiloride hydrochloride)

Cat. No.: HY-101840A Purity: 99.92%
COA Handling Instructions

EIPA hydrochloride (L593754 hydrochloride) est un inhibiteur de canal TRPP3 avec un IC50 de 10,5 μM. EIPA hydrochloride inhibe également les échangeurs Na+/H+ (NHE) et macropinocytose.

EIPA (L593754) hydrochloride is an orally active TRPP3 channel inhibitor with an IC50 of 10.5 μM. EIPA hydrochloride also enhances autophagy by inhibiting Na+/H+-exchanger 3 (NHE3). EIPA hydrochloride inhibits macropinocytosis as well. EIPA hydrochloride can be used in the research of inflammation and cancers, such as gastric cancer, colon carcinoma, pancreatic carcinoma.

For research use only. We do not sell to patients.

EIPA hydrochloride Chemical Structure

EIPA hydrochloride Chemical Structure

CAS No. : 1345839-28-2

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Customer Review

Based on 35 publication(s) in Google Scholar

Other Forms of EIPA hydrochloride:

Top Publications Citing Use of Products

35 Publications Citing Use of MCE EIPA hydrochloride

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Description

EIPA (L593754) hydrochloride is an orally active TRPP3 channel inhibitor with an IC50 of 10.5 μM. EIPA hydrochloride also enhances autophagy by inhibiting Na+/H+-exchanger 3 (NHE3). EIPA hydrochloride inhibits macropinocytosis as well. EIPA hydrochloride can be used in the research of inflammation and cancers, such as gastric cancer, colon carcinoma, pancreatic carcinoma[1][2][3][5].

IC50 & Target[7]

COX-2

 

In Vitro

EIPA hydrochloride (100 μM, 30 min) suppresses TRPP3-mediated Ca2+ uptake in X. laevis oocytes[1].
EIPA hydrochloride (10-100 μM) reversibly inhibits the basal Na+ current (IC50: 19.5 μM)[1].
EIPA hydrochloride (300 μM, 6h) enhances autophagy through NHE3 (Na+/H+-exchanger 3) in IEC-18 cells[2].
EIPA hydrochloride (20 μM, 2 h) blocks macropinocytosis-mediated uptake of CA-PZ massively entry in HT-29 cells and MIA PaCa-2 cells[3].
EIPA hydrochloride (30 μM, 3h) attenuates Zinc/Kainate toxicity by decreasing Zn2+ entry in cerebellar granule neurons[4].
EIPA hydrochloride (5-100 μM, 48h) suppresses proliferation of MKN28 cells through up-regulation of p21 expression[5].
EIPA hydrochloride (3 μM, 6 h) inhibits the LPS-induced increase in the level of COX-2 protein[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay[5]

Cell Line: MKN28 cells
Concentration: 5, 10, 25, 50, and 100 μM
Incubation Time: 48 h
Result: Inhibited cell proliferation in a dose- and time-dependent manner.

Western Blot Analysis[2]

Cell Line: IEC-18 cells
Concentration: 300 μM
Incubation Time: 6 h
Result: Increased total LC3-II protein levels and P62 flux.
Increased ATG5, 7, 12 and P62 expression.
In Vivo

EIPA hydrochloride (Intravenous injection, 1 mg/kg) dose-dependently attenuates the I/R (Ischemia/reperfusion)-induced renal dysfunction in ddY strain mice[6].
EIPA hydrochloride (oral administration, 10 mg/kg) inhibits LPS-induced inflammation in air pouch-type LPS-induced inflammation model[7].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ddY strain mice[6]
Dosage: 1 mg/kg
Administration: Intravenous injection
Result: Attenuated histologic renal damage, and imprved the I/R-induced increases in renal ET-1 contents.
Animal Model: Air pouch-type LPS-induced inflammation model[7]
Dosage: 10 mg/kg
Administration: Oral administration
Result: Inhibited the LPS-induced infiltration of leukocytes into the pouch.
Inhibited the amount of PGE2 in the pouch fluid.
Molecular Weight

336.22

Appearance

Solid

Formula

C11H19Cl2N7O
CAS No.
SMILES

O=C(C1=NC(Cl)=C(N(CC)C(C)C)N=C1N)NC(N)=N.Cl
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Solvent & Solubility
In Vitro: 

DMSO : 130 mg/mL (386.65 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9742 mL 14.8712 mL 29.7424 mL
5 mM 0.5948 mL 2.9742 mL 5.9485 mL
10 mM 0.2974 mL 1.4871 mL 2.9742 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation
References
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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Keywords:

EIPA1345839-28-2L593754MH 12-43EthylisopropylamilorideL 593754L-593754TRP ChannelAutophagyCOXProstaglandin ReceptorNa+/H+ Exchanger (NHE)Transient receptor potential channelsCyclooxygenaseSodium/hydrogen ExchangerSodium-hydrogen ExchangerCation channelNHE3IECsIBDsIEC-18neuronzinccalciumkainiteMKN28gastric cancercolon carcinomapancreatic carcinomarenal failureddY strain miceInhibitorinhibitorinhibit

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