EIPA hydrochloride (Synonyms: L593754 hydrochloride; MH 12-43 hydrochloride; Ethylisopropylamiloride hydrochloride)

Name: EIPA hydrochloride
Brand: MedChemExpress
SKU: HY-101840A
Rating: 5.0 (98 reviews)

Cat. No.: HY-101840A Purity: 98.01%: Data Sheet
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EIPA (L593754) hydrochloride is an orally active TRPP3 channel inhibitor with an IC₅₀ of 10.5 μM. EIPA hydrochloride also enhances autophagy by inhibiting Na⁺/H⁺-exchanger 3 (NHE3). EIPA hydrochloride inhibits macropinocytosis as well. EIPA hydrochloride can be used in the research of inflammation and cancers, such as gastric cancer, colon carcinoma, pancreatic carcinoma.

For research use only. We do not sell to patients.

CAS No. : 1345839-28-2

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 98 publication(s) in Google Scholar

Other Forms of EIPA hydrochloride:

EIPA In-stock
EIPA hydrochloride (Standard) Get quote

98 Publications Citing Use of MCE EIPA hydrochloride

Gel Electrophoresis

RT-PCR

Flow Cytometry

Cell Imaging/Staining

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Jul 15:S1535-6108(25)00271-5. [Abstract]; Murine CAFs were cultured in 4 mM or 0.2 mM Q with DMSO or 25 μM EIPA hydrochloride for 4 h. The protein levels of sestrin2 (SESN2) were analyzed by western blot.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Cell. 2025 Jul 15:S1535-6108(25)00271-5. [Abstract]; Murine CAFs were cultured in 4 mM or 0.2 mM Q with DMSO or 25 μM EIPA for 32 h.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Mater. 2025 Jan 10:e2415030. [Abstract]; EIPA (50 μM, 7 h) decreased the internalization efficiency of F/G/H-GNC in 4T1 cells by approximately 17%.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Cancer Res. 2025 Oct 9. [Abstract]; EIPA (30 μM, 24 h) could not completely block the signal enhancement of FITC-EV mediated by P-3 Fax-Neu5Ac in RT-112 and KU-19-19 cells.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: J Cell Biol. 2025 May 5;224(5):e202405060. [Abstract]; Agarose gel of Xbp1 cDNA amplicons in AML12 treated with Ctrl/CM ± endocytosis inhibitors (amiloride, nocodazole, nystatin, EIPA hydrochloride (40 μM)).

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2025 Feb;20(2):296-302. [Abstract]; EIPA (5 μg/mL, 24 h) could not reverse 500 nM NTZ-NPs induced EGFR protein degradation in MDA-MB-231 cells.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Cell Metab. 2022 Dec 6;34(12):2018-2035.e8. [Abstract]; Bone marrow cells cultured in medium containing 10 ng/mL GM-CSF for 3 days were treated with 2 μg PKH67-labeled sEVs with or without Amiloride (10 μM), Chlorpromazine (10 μM), or EIPA (5 μM) for 1 h. The median fluorescence intensity (MFI) of PKH67 was assessed by flow cytometry.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Emerg Microbes Infect. 2022 Dec;11(1):1135-1144. [Abstract]; The blocking effect of EIPA hydrochloride (30 μM) on dextran, CPZ (25 μM) on transferrin, and MCD (3 mM) or filipin III (1 μg/ml) on CTB was observed in Vero E6 cells. Hoechst was used to stain the nuclei.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 2021 Apr 1.; EIPA (100 μM, 5 h) inhibited the intracellular uptake of antigenic peptides GT83-Mn²⁺/OVA257-280-FITC NPs in DC2.4 cells.

: EIPA hydrochloride purchased from MedChemExpress. Usage Cited in: Mol Pharm. 2021 Oct 4;18(10):3750-3762. [Abstract]; The cellular fluorescence profiles showed 5-TAMRA-RRL signals were sharply inhibited by Pitstop 2, EIPA hydrochloride (100 μM) and VER-155008 (20 μM).

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Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[7]

COX-2

In Vitro

EIPA hydrochloride (100 μM, 30 min) suppresses TRPP3-mediated Ca²⁺ uptake in X. laevis oocytes^[1].
EIPA hydrochloride (10-100 μM) reversibly inhibits the basal Na⁺ current (IC₅₀: 19.5 μM)^[1].
EIPA hydrochloride (300 μM, 6h) enhances autophagy through NHE3 (Na⁺/H⁺-exchanger 3) in IEC-18 cells^[2].
EIPA hydrochloride (20 μM, 2 h) blocks macropinocytosis-mediated uptake of CA-PZ massively entry in HT-29 cells and MIA PaCa-2 cells^[3].
EIPA hydrochloride (30 μM, 3h) attenuates Zinc/Kainate toxicity by decreasing Zn²⁺ entry in cerebellar granule neurons^[4].
EIPA hydrochloride (5-100 μM, 48h) suppresses proliferation of MKN28 cells through up-regulation of p21 expression^[5].
EIPA hydrochloride (3 μM, 6 h) inhibits the LPS-induced increase in the level of COX-2 protein^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[5]

Cell Line:	MKN28 cells
Concentration:	5, 10, 25, 50, and 100 μM
Incubation Time:	48 h
Result:	Inhibited cell proliferation in a dose- and time-dependent manner.

Western Blot Analysis^[2]

Cell Line:	IEC-18 cells
Concentration:	300 μM
Incubation Time:	6 h
Result:	Increased total LC3-II protein levels and P62 flux. Increased ATG5, 7, 12 and P62 expression.

In Vivo

EIPA hydrochloride (Intravenous injection, 1 mg/kg) dose-dependently attenuates the I/R (Ischemia/reperfusion)-induced renal dysfunction in ddY strain mice^[6].
EIPA hydrochloride (oral administration, 10 mg/kg) inhibits LPS-induced inflammation in air pouch-type LPS-induced inflammation model^[7].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ddY strain mice^[6]
Dosage:	1 mg/kg
Administration:	Intravenous injection
Result:	Attenuated histologic renal damage, and imprved the I/R-induced increases in renal ET-1 contents.

Animal Model:	Air pouch-type LPS-induced inflammation model^[7]
Dosage:	10 mg/kg
Administration:	Oral administration
Result:	Inhibited the LPS-induced infiltration of leukocytes into the pouch. Inhibited the amount of PGE2 in the pouch fluid.

Molecular Weight

336.22

Formula

C₁₁H₁₉Cl₂N₇O

CAS No.

1345839-28-2

Appearance

Solid

Color

Light yellow to yellow

SMILES

O=C(C1=NC(Cl)=C(N(CC)C(C)C)N=C1N)NC(N)=N.Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : 130 mg/mL (386.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.9742 mL	14.8712 mL	29.7424 mL
5 mM	0.5948 mL	2.9742 mL	5.9485 mL
10 mM	0.2974 mL	1.4871 mL	2.9742 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.01%

Select Batch:

Data Sheet (279 KB) SDS (254 KB)

COA (248 KB) RP-HPLC (259 KB) MS (69 KB)

Handling Instructions (2659 KB)

References

[1]. Dai XQ, et al. Inhibition of TRPP3 channel by MK-870 and analogs. Mol Pharmacol. 2007 Dec;72(6):1576-85. [Content Brief]

[2]. Shi H, et al. Na+/H+ Exchanger Regulates Amino Acid-Mediated Autophagy in Intestinal Epithelial Cells. Cell Physiol Biochem. 2017;42(6):2418-2429. [Content Brief]

[3]. Zhu BY, et al. A new HDAC inhibitor cinnamoylphenazine shows antitumor activity in association with intensive macropinocytosis. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.9742 mL	14.8712 mL	29.7424 mL	74.3561 mL
	5 mM	0.5948 mL	2.9742 mL	5.9485 mL	14.8712 mL
	10 mM	0.2974 mL	1.4871 mL	2.9742 mL	7.4356 mL
	15 mM	0.1983 mL	0.9914 mL	1.9828 mL	4.9571 mL
	20 mM	0.1487 mL	0.7436 mL	1.4871 mL	3.7178 mL
	25 mM	0.1190 mL	0.5948 mL	1.1897 mL	2.9742 mL
	30 mM	0.0991 mL	0.4957 mL	0.9914 mL	2.4785 mL
	40 mM	0.0744 mL	0.3718 mL	0.7436 mL	1.8589 mL
	50 mM	0.0595 mL	0.2974 mL	0.5948 mL	1.4871 mL
	60 mM	0.0496 mL	0.2479 mL	0.4957 mL	1.2393 mL
	80 mM	0.0372 mL	0.1859 mL	0.3718 mL	0.9295 mL
	100 mM	0.0297 mL	0.1487 mL	0.2974 mL	0.7436 mL

EIPA hydrochloride Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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EIPA hydrochloride (Synonyms: L593754 hydrochloride; MH 12-43 hydrochloride; Ethylisopropylamiloride hydrochloride)

Customer Review

Other Forms of EIPA hydrochloride:

EIPA hydrochloride Related Antibodies

98 Publications Citing Use of MCE EIPA hydrochloride

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All TRP Channel Isoform Specific Products:

View All COX Isoform Specific Products:

View All Prostaglandin Receptor Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

EIPA hydrochloride Related Antibodies

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Dilution Calculator

Complete Stock Solution Preparation Table

EIPA hydrochloride Related Classifications

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