2. Apoptosis
  3. Caspase
  4. Ac-LEHD-AFC

Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase.

For research use only. We do not sell to patients.

Ac-LEHD-AFC Chemical Structure

Ac-LEHD-AFC Chemical Structure

CAS No. : 210345-03-2

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10 mg USD 210 In-stock
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Based on 1 publication(s) in Google Scholar

Ac-LEHD-AFC Related Antibodies

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Description

Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].

IC50 & Target

Caspase-9[1]
In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The assessment of the caspase activity by fluorogenic substrate assay[1]:
1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) .
2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM).
3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Ac-LEHD-AFC Related Antibodies
Molecular Weight

765.69

Formula

C33H38F3N7O11
CAS No.
Appearance

Solid

Color

White to off-white

Emission (Em)

454
Excitation (Ex)

445
SMILES

FC(C(C1=CC=C(C=C1O2)NC([C@@H](NC([C@H](CC3=CNC=N3)NC([C@@H](NC([C@H](CC(C)C)NC(C)=O)=O)CCC(O)=O)=O)=O)CC(O)=O)=O)=CC2=O)(F)F
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (130.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3060 mL 6.5301 mL 13.0601 mL
5 mM 0.2612 mL 1.3060 mL 2.6120 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
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Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

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Volume (start)

V1

=
Concentration (final)

C2

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Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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mg/kg

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g

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(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3060 mL 6.5301 mL 13.0601 mL 32.6503 mL
5 mM 0.2612 mL 1.3060 mL 2.6120 mL 6.5301 mL
10 mM 0.1306 mL 0.6530 mL 1.3060 mL 3.2650 mL
15 mM 0.0871 mL 0.4353 mL 0.8707 mL 2.1767 mL
20 mM 0.0653 mL 0.3265 mL 0.6530 mL 1.6325 mL
25 mM 0.0522 mL 0.2612 mL 0.5224 mL 1.3060 mL
30 mM 0.0435 mL 0.2177 mL 0.4353 mL 1.0883 mL
40 mM 0.0327 mL 0.1633 mL 0.3265 mL 0.8163 mL
50 mM 0.0261 mL 0.1306 mL 0.2612 mL 0.6530 mL
60 mM 0.0218 mL 0.1088 mL 0.2177 mL 0.5442 mL
80 mM 0.0163 mL 0.0816 mL 0.1633 mL 0.4081 mL
100 mM 0.0131 mL 0.0653 mL 0.1306 mL 0.3265 mL
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Ac-LEHD-AFC Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Ac-LEHD-AFC210345-03-2CaspaseInhibitorinhibitorinhibit

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