Ac-LEHD-AFC
Based on 3 publication(s) in Google Scholar
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase.
For research use only. We do not sell to patients.
- Purity: 99.95%
- CAS No.: 210345-03-2
- Formula: C33H38F3N7O11
- Molecular Weight:765.69
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Ac-LEHD-AFC
MoreAll Caspase Isoforms
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Biological Activity
Caspase-9[1]
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
The assessment of the caspase activity by fluorogenic substrate assay[1]:
1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) .
2. Incubate the lysis buffer (25 mg of total protein) with 50 mM of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM).
3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 210345-03-2
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Appearance Solid
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Molecular Weight 765.69
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Formula C33H38F3N7O11
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Color White to off-white
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SMILES
FC(C(C1=CC=C(C=C1O2)NC([C@@H](NC([C@H](CC3=CNC=N3)NC([C@@H](NC([C@H](CC(C)C)NC(C)=O)=O)CCC(O)=O)=O)=O)CC(O)=O)=O)=CC2=O)(F)F
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Publications (3)
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Journal Impact Factor
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Most Recent
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Cell Death Dis
GSDMEa-mediated pyroptosis is bi-directionally regulated by caspase and required for effective bacterial clearance in teleost. [Abstract]2022 May 24;13(5):491. PMID: 35610210 -
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Genes (Basel)
Characterization of the Apoptotic and Antimicrobial Activities of Two Initiator Caspases of Sea Cucumber Apostichopus japonicus. [Abstract]2024 Apr 25;15(5):540. PMID: 38790170
Solvent & Solubility
DMSO : ≥ 100 mg/mL (130.60 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3060 mL | 6.5301 mL | 13.0601 mL | 32.6503 mL |
| 5 mM | 0.2612 mL | 1.3060 mL | 2.6120 mL | 6.5301 mL | |
| 10 mM | 0.1306 mL | 0.6530 mL | 1.3060 mL | 3.2650 mL | |
| 15 mM | 0.0871 mL | 0.4353 mL | 0.8707 mL | 2.1767 mL | |
| 20 mM | 0.0653 mL | 0.3265 mL | 0.6530 mL | 1.6325 mL | |
| 25 mM | 0.0522 mL | 0.2612 mL | 0.5224 mL | 1.3060 mL | |
| 30 mM | 0.0435 mL | 0.2177 mL | 0.4353 mL | 1.0883 mL | |
| 40 mM | 0.0327 mL | 0.1633 mL | 0.3265 mL | 0.8163 mL | |
| 50 mM | 0.0261 mL | 0.1306 mL | 0.2612 mL | 0.6530 mL | |
| 60 mM | 0.0218 mL | 0.1088 mL | 0.2177 mL | 0.5442 mL | |
| 80 mM | 0.0163 mL | 0.0816 mL | 0.1633 mL | 0.4081 mL | |
| 100 mM | 0.0131 mL | 0.0653 mL | 0.1306 mL | 0.3265 mL |
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