1. Metabolic Enzyme/Protease
  2. Cathepsin
  3. PMSF

PMSF  (Synonyms: Phenylmethylsulfonyl fluoride; Benzylsulfonyl fluoride)

Cat. No.: HY-B0496 Purity: 99.91%
COA Handling Instructions

PMSF is an irreversible serine/cysteine protease inhibitor commonly used in the preparation of cell lysates.

For research use only. We do not sell to patients.

PMSF Chemical Structure

PMSF Chemical Structure

CAS No. : 329-98-6

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10 mM * 1 mL in DMSO
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500 mg USD 66 In-stock
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Customer Review

Based on 61 publication(s) in Google Scholar

Top Publications Citing Use of Products

61 Publications Citing Use of MCE PMSF

  • Biological Activity

  • Protocol

  • Purity & Documentation

  • References

  • Customer Review

Description

PMSF is an irreversible serine/cysteine protease inhibitor commonly used in the preparation of cell lysates.

In Vitro

PMSF (2 mM) inhibits carbachol-stimulated inositol phosphate accumulation in the presence of Li+ by only 15%-19%. PMSF inhibition of phosphoinositide turnover is due to one or more steps following phosphoinositide breakdown[1]. PMSF inhibits the acylation of the inositol residue of GPI intermediates in bloodstream-form T. brucei. PMSF inhibits the formation of glycolipid C but does not inhibit fatty acid remodeling in vitro. PMSF inhibits GPI acylation and ethanolamine phosphate addition in procyclic trypanosomes but not in Hela cells[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

PMSF (0.1 mL/10 g b.wt, i.p.) produces antinociception as indicated by the dose-responsive increase in % MPE in the tail-flick latency evaluation, but fails to produce a clear dose-responsive inhibition of locomotion. Mice receiving i.p. injections of PMSF exhibit cannabinoid effects that includes antinociception, hypothermia and immobility with ED50 values of 86, 224 and 206 mg/kg, respectively. PMSF (30 mg/kg) pretreatment potentiates the effects of anandamide on tail-flick response (antinociception), spontaneous activity and mobility by 5-, 10- and 8-fold, respectively[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

174.19

Formula

C7H7FO2S

CAS No.
Appearance

Solid

Color

White to off-white

SMILES

O=S(CC1=CC=CC=C1)(F)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 250 mg/mL (1435.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Isopropanol : 60 mg/mL (344.45 mM; Need ultrasonic)

H2O : < 0.1 mg/mL (insoluble)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 5.7409 mL 28.7043 mL 57.4086 mL
5 mM 1.1482 mL 5.7409 mL 11.4817 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

    Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

    Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
  • Protocol 2

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

    Solubility: ≥ 2 mg/mL (11.48 mM); Clear solution

    This protocol yields a clear solution of ≥ 2 mg/mL (saturation unknown).

    Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

    Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

  • Protocol 1

    Add each solvent one by one:  Corn Oil

    Solubility: 20 mg/mL (114.82 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
%
DMSO +
+
%
Tween-80 +
%
Saline
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Calculation results:
Working solution concentration: mg/mL
Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
 If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation

Purity: 99.91%

References
Animal Administration
[3]

Male ICR mice weighing 18 to 25 g are used in the assay. PMSF is dissolved in sesame oil and administered i.p. at a volume of 0.1 mL/10 g b.wt. PMSF is always administered 10 min before i.v. anandamide or vehicle injections. Mice are acclimated to the evaluation room overnight without interruption of food or water. After i.v. anandamide or vehicle administration each animal is evaluated as follows: tail-flick latency (antinociception) response at 5 min and spontaneous (locomotor) activity at 5 to 15 min; or core (rectal) temperature at 5 min and ring-immobility (catalepsy) at 5 to 10 min.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
Isopropanol / DMSO 1 mM 5.7409 mL 28.7043 mL 57.4086 mL 143.5214 mL
5 mM 1.1482 mL 5.7409 mL 11.4817 mL 28.7043 mL
10 mM 0.5741 mL 2.8704 mL 5.7409 mL 14.3521 mL
15 mM 0.3827 mL 1.9136 mL 3.8272 mL 9.5681 mL
20 mM 0.2870 mL 1.4352 mL 2.8704 mL 7.1761 mL
25 mM 0.2296 mL 1.1482 mL 2.2963 mL 5.7409 mL
30 mM 0.1914 mL 0.9568 mL 1.9136 mL 4.7840 mL
40 mM 0.1435 mL 0.7176 mL 1.4352 mL 3.5880 mL
50 mM 0.1148 mL 0.5741 mL 1.1482 mL 2.8704 mL
60 mM 0.0957 mL 0.4784 mL 0.9568 mL 2.3920 mL
80 mM 0.0718 mL 0.3588 mL 0.7176 mL 1.7940 mL
100 mM 0.0574 mL 0.2870 mL 0.5741 mL 1.4352 mL
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PMSF Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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PMSF
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