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Pyranine  (Synonyms: HPTS; Solvent Green 7)

Cat. No.: HY-D0023 Purity: 98.0%
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Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu+ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm).

For research use only. We do not sell to patients.

CAS No. : 6358-69-6

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Based on 2 publication(s) in Google Scholar

Other Forms of Pyranine:

Pyranine Related Antibodies

Top Publications Citing Use of Products

2 Publications Citing Use of MCE Pyranine

Bio/Physico-chemical Assay

    Pyranine purchased from MedChemExpress. Usage Cited in: Food Biosci. 2025 Jul.

    Proteoliposomes were diluted to 1 mg/mL lipid concentration with external buffer containing 1 μM Pyranine. SH or palmitic acid was added as indicated. Valinomycin was included to increase K+ permeability, generating a membrane potential that facilitated UCP1- mediated proton transport.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Pyranine (HPTS; Solvent Green 7) is a cell-impermeant pH-sensitive fluorescent indicator. Pyranine acts as a class of fluorescent chemosensor for the Cu+ ion. Pyranine is used as a coloring agent, biological stain, optical detecting reagent, and pH indicator (Ex/Em = 450/510 nm)[1][2][3].

    In Vitro

    Pyranine is capable of discriminating ranges of cations from the Cu+ ion, even in competing environment. Pyranine displays a rapid fluorescence response (t1/2=1.66 min) towards the Cu+ ion, and the micromolar detection limit enables the detection of the ion in environmental samples. The observed stoichiometry of complexation between Pyranine and Cu+ is 2:1[1].
    The pH-sensitive fluorescent indicator Pyranine is studied to determine its usefulness as probes for the living cornea and anterior chamber. Adequate concentrations are reached in the cornea and anterior chamber after topical administration; measurements can be made by fluorophotometry for many hours. The pH is calculated by measuring the ratio of fluorescent intensity at two excitation wavelengths, I463/I404, a measurement which is dependent on pH but independent of the concentration of the fluorophore and other variables which can alter the intensity. In the rabbit eye, Pyranine in the cornea and anterior chamber is observed to undergo easily measurable changes in fluorescent ratios associated with lid closure and contact lens wear, indicating its sensitivity to mild changes in pH[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Pyranine Related Antibodies
    Molecular Weight

    524.39

    Formula

    C16H7Na3O10S3
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    Emission (Em)

    510
    Excitation (Ex)

    450
    SMILES

    O=S(C1=C(C2=C34)C=CC4=C(O)C=C(S(=O)(O[Na])=O)C3=CC=C2C(S(=O)(O[Na])=O)=C1)(O[Na])=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, sealed storage, away from moisture and light

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

    Solvent & Solubility
    In Vitro: 

    H2O : 20 mg/mL (38.14 mM; ultrasonic and warming and heat to 60°C)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.9070 mL 9.5349 mL 19.0698 mL
    5 mM 0.3814 mL 1.9070 mL 3.8140 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    This equation is commonly abbreviated as: C1V1 = C2V2

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    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

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    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
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    Working solution concentration: mg/mL
    This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
    Purity & Documentation

    Purity: 98.0%

    SDS (393 KB)

    COA (260 KB) HNMR (269 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [3]

    PAAm-κC composite gels are prepared by free-radical copolymerization using 0.71 g of AAm, 0.01 g of BIS (N, N’-methylenebisacrylamide), 0.008 g of APS (ammonium persulfate) and 2 µl of TEMED (tetramethylethylenediamine) are dissolved in 5 mL of distilled water (pH 6.5) by heating. The heated mixture solution is held at 80°C. Then different amounts of κ-carrageenan (0.5, 1, 1.5, 2, 2.5 and 3 (w/v) % κC) are added. Pyranine (Py) is used in the PAAm–κC composites as a fluorescence probe, which is a derivative of pyrene possessing three SO3- groups, which can form bonds with positive charges on the gel. Pyranine (Py) concentration is kept constant at 4×10-4 M, for all experiments. The solution is stirred (200 rpm) for 15 min to achieve a homogenous sample solution. All samples are deoxygenated by bubbling nitrogen for 10 min just before the polymerization process. The drying and swelling experiments of disc-shaped PAAm–κC composite gels prepared are performed in air and in water, respectively, at various temperatures (30, 40, 50, and 60°C). A model LS-50 spectrometer equipped with a temperature controller is used for fluorescence intensity measurements, which are made at a 90° position and spectral bandwidths are kept at 5 nm. Disc-shaped gel samples are placed on the wall of a 1-cm path-length square quartz cell filled with air and/or water for the drying and swelling experiments[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    H2O 1 mM 1.9070 mL 9.5349 mL 19.0698 mL 47.6744 mL
    5 mM 0.3814 mL 1.9070 mL 3.8140 mL 9.5349 mL
    10 mM 0.1907 mL 0.9535 mL 1.9070 mL 4.7674 mL
    15 mM 0.1271 mL 0.6357 mL 1.2713 mL 3.1783 mL
    20 mM 0.0953 mL 0.4767 mL 0.9535 mL 2.3837 mL
    25 mM 0.0763 mL 0.3814 mL 0.7628 mL 1.9070 mL
    30 mM 0.0636 mL 0.3178 mL 0.6357 mL 1.5891 mL

    * Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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    Pyranine Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Pyranine6358-69-6HPTS Solvent Green 7Solvent Green7Solvent Green 7Solvent Green-7Fluorescent DyeEnvironmental PollutantsEnvironmental ContaminantspHfluorescentindicatorCu+Inhibitorinhibitorinhibit

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