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  4. Monochlorobimane

Monochlorobimane  (Synonyms: Chlorobimane，mBBr)

Cat. No.: HY-101899 Purity: 99.0%
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Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays.

For research use only. We do not sell to patients.

CAS No. : 76421-73-3

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
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Customer Review

Based on 17 publication(s) in Google Scholar

Other Forms of Monochlorobimane:

Monochlorobimane Related Antibodies

Top Publications Citing Use of Products

    Monochlorobimane purchased from MedChemExpress. Usage Cited in: Biomaterials. 2025 Feb 12:318:123188.  [Abstract]

    Monochlorobimane (40 μM, 37°C, 20 min) was used to measure GSH levels in HepG2 cells.

    Monochlorobimane purchased from MedChemExpress. Usage Cited in: Ecotoxicol Environ Saf. 2025 Jun 4:300:118461.  [Abstract]

    Monochlorobimane (20–100 μM, 2 h) was used to determine intracellular GSH levels in oocytes.

    Monochlorobimane purchased from MedChemExpress. Usage Cited in: Antioxidants (Basel). 2024 Jun 29;13(7):798.  [Abstract]

    Monochlorobimane (mCB, 5 μM, diluted in Hanks' Balanced Salt Solution (HBSS) for 4 h) was used to assess glutathione (GSH) levels in human skin fibroblasts.

    Monochlorobimane purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2023 Jul 28;24(15):12104.

    MCB (5 μM, diluted in Hanks' balanced salt solution (HBSS) for 4 h) was used to assess glutathione (GSH) levels in human skin fibroblasts.

    Monochlorobimane purchased from MedChemExpress. Usage Cited in: Int J Biol Sci. 2022 Jul 11;18(11):4545-4559.  [Abstract]

    Intracellular GSH levels in the retinal tissues of EAU mice were quantified using monochlorobimane (30 min).

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Monochlorobimane (Chlorobimane) is a fluorescent dye (λex=380 nm, λem=470 nm) to measure glutathione (GSH) in cellular assays[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Solution preparation[3]
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 10 mM.
    1.2 Preparation of working solution
    Dilute to 1-10 μM with PBS or cell culture medium (optimized according to the experiment).
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 Cells are plated in clear-bottomed black 96-well microplates.
    2.2 Add Monochlorobimane working solution and incubate at 37°C for 30 minutes (optimized according to the experiment).
    2.3 The fluorescence intensity is measured with fluorescence microscopy or Fluorescence microplate reader (Ex/Em = 380/480 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Monochlorobimane Related Antibodies
    Molecular Weight

    226.66

    Formula

    C10H11ClN2O2
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    Emission (Em)

    490
    Excitation (Ex)

    395
    SMILES

    O=C1N(C(C(C)=C2C)=O)N2C(CCl)=C1C
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    -20°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 50 mg/mL (220.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 4.4119 mL 22.0595 mL 44.1189 mL
    5 mM 0.8824 mL 4.4119 mL 8.8238 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: 99.86%

    SDS (453 KB)

    COA (256 KB) HNMR (216 KB) LCMS (95 KB)

    Handling Instructions (2659 KB)
    Dyeing Example
    References
    Kinase Assay
    [1]

    Monochlorobimane (mCB) is added to the second half liver tissue homogenate to a final concentration of 100 mM along with glutathione S-transferase (1 U/mL) obtained from equine liver; the homogenate is then allowed to incubate at room temperature for 30 min. The Glutathione (GSH)-Monochlorobimane adduct is measured in a microtiter reader[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 4.4119 mL 22.0595 mL 44.1189 mL 110.2974 mL
    5 mM 0.8824 mL 4.4119 mL 8.8238 mL 22.0595 mL
    10 mM 0.4412 mL 2.2059 mL 4.4119 mL 11.0297 mL
    15 mM 0.2941 mL 1.4706 mL 2.9413 mL 7.3532 mL
    20 mM 0.2206 mL 1.1030 mL 2.2059 mL 5.5149 mL
    25 mM 0.1765 mL 0.8824 mL 1.7648 mL 4.4119 mL
    30 mM 0.1471 mL 0.7353 mL 1.4706 mL 3.6766 mL
    40 mM 0.1103 mL 0.5515 mL 1.1030 mL 2.7574 mL
    50 mM 0.0882 mL 0.4412 mL 0.8824 mL 2.2059 mL
    60 mM 0.0735 mL 0.3677 mL 0.7353 mL 1.8383 mL
    80 mM 0.0551 mL 0.2757 mL 0.5515 mL 1.3787 mL
    100 mM 0.0441 mL 0.2206 mL 0.4412 mL 1.1030 mL
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    Monochlorobimane Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Monochlorobimane76421-73-3Chlorobimane，mBBrFluorescent DyeInhibitorinhibitorinhibit

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