1. Dye Reagents

Monochlorobimane (Synonyms: Chlorobimane)

Cat. No.: HY-101899
Handling Instructions

Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm).

For research use only. We do not sell to patients.

Monochlorobimane Chemical Structure

Monochlorobimane Chemical Structure

CAS No. : 76421-73-3

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Monochlorobimane is a fluorescent dye (λex=380 nm, λem=470 nm).

In Vitro

A common technique to measure Glutathione (GSH) in cultured cells is to add Monochlorobimane to the culture medium where it readily enters cells to form a fluorescent GSH-monochlorobimane adduct that can be measured fluorometrically. It is clear that the Monochlorobimane approach gives comparable results to the HPLC technique. Although most laboratories use 100 mM Monochlorobimane in their assay our results show that the Monochlorobimane concentration can be lowered to 25 mM and still accurately measure homogenate GSH content[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 4.4119 mL 22.0595 mL 44.1189 mL
5 mM 0.8824 mL 4.4119 mL 8.8238 mL
10 mM 0.4412 mL 2.2059 mL 4.4119 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Kinase Assay
[1]

Monochlorobimane (mCB) is added to the second half liver tissue homogenate to a final concentration of 100 mM along with glutathione S-transferase (1 U/mL) obtained from equine liver; the homogenate is then allowed to incubate at room temperature for 30 min. The Glutathione (GSH)-Monochlorobimane adduct is measured in a microtiter reader[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

226.66

Formula

C₁₀H₁₁ClN₂O₂

CAS No.

76421-73-3

SMILES

O=C1N(C(C(C)=C2C)=O)N2C(CCl)=C1C

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Shipping

Room temperature in continental US; may vary elsewhere

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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Product Name:
Monochlorobimane
Cat. No.:
HY-101899
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Monochlorobimane

Cat. No.: HY-101899