CellTracker Blue CMF2HC Dye

Name: CellTracker Blue CMF2HC Dye
Brand: MedChemExpress
SKU: HY-D1571
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1571 Purity: 99.78%: FAQs
Data Sheet SDS COA Handling Instructions

Molarity Calculator

CellTracker Blue CMF2HC Dye is a blue dye, can be used in two-channel nuclei acid sequencing, with blue and purple excitation light (450-460 nm/400-405nm or 415-450 nm/480-525nm). CellTracker Blue CMF2HC Dye can be used to rapid determination of antibiotic sensitivity of microorganisms.

For research use only. We do not sell to patients.

CellTracker Blue CMF2HC Dye Chemical Structure

CAS No. : 215868-45-4

Size	Price	Stock	Quantity
1 mg	USD 114	In-stock	Estimated Time of Arrival: December 31
5 mg	USD 330	In-stock	Estimated Time of Arrival: December 31
10 mg	USD 500	In-stock	Estimated Time of Arrival: December 31
25 mg	USD 900	In-stock	Estimated Time of Arrival: December 31
50 mg		Get quote
100 mg		Get quote

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Purity & Documentation
References
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Description

In Vitro

CellTracker Blue CMF2HC Dye is a blue dye that can be excitable by a blue light source having a wavelength of about 450-460 nm, is used as the first or the second detectable label described herein^[1].
Blue/Violet Two-Channel Sequencing Methods^[1]:
1.Contacting a primer polynucleotide/taiget polynucleotide complex with a mixture comprising one or more of a first type of nucleotide, a second type of nucleotide, a third type of nucleotide, and a fourth type of nucleotide, wherein the primer polynucleotide is complementary to at least a portion ofthe single stranded target polynucleotide;
2.Incorporating one type of nucleotide from the mixture to the primer polynucleotide to produce an extended primer polynucleotide (i.e., an extended primer polynucleotide/target polynucleotide complex);
3.Performing a first imaging event using a first excitation light source and collecting a first emission signal from the extended primer polynucleotide/target polynucleotide complex with a first emission filer;
4.Performing a second imaging event using a second excitation light source and collecting a second emission signal from the extended primer polynucleotide/taiget polynucleotide complex with a second emission filter;
Note^[1]:
a. one of the first excitation light source and the second excitation light source has a wavelength of about 350 nm to about 410 nm, and the other one of the first excitation light source and the second excitation light source has a wavelength of about 450 nm to about 460 nm;
b. one of the first emission filter and the second emission filter has a detection wavelength of about 415 nm to about 450 nm, and the other one of the first emission filter and the second emission filter has a detection wavelength of about 480 nm to about 525 nm.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

246.59

Formula

C₁₀H₅ClF₂O₃

CAS No.

215868-45-4

Appearance

Solid

Color

White to off-white

Emission (Em)

451

Excitation (Ex)

405

SMILES

FC1=C2C(C(CCl)=CC(O2)=O)=CC(F)=C1O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (506.91 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	4.0553 mL	20.2766 mL	40.5531 mL
5 mM	0.8111 mL	4.0553 mL	8.1106 mL
10 mM	0.4055 mL	2.0277 mL	4.0553 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.78%

Select Batch:

Data Sheet (274 KB) SDS (251 KB)

COA (250 KB) HNMR (248 KB) LCMS (97 KB)

Handling Instructions (2659 KB)

References

[1]. Wu Xiaolin, et al. Methods and compositions for nucleic acid sequencing: US, US20220195518[P]. 2022-06-23.

[2]. Super Michael, et al. Rapid antibiotic susceptibility testing: US, US20150064703[p]. 2015-03-05.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	4.0553 mL	20.2766 mL	40.5531 mL	101.3829 mL
	5 mM	0.8111 mL	4.0553 mL	8.1106 mL	20.2766 mL
	10 mM	0.4055 mL	2.0277 mL	4.0553 mL	10.1383 mL
	15 mM	0.2704 mL	1.3518 mL	2.7035 mL	6.7589 mL
	20 mM	0.2028 mL	1.0138 mL	2.0277 mL	5.0691 mL
	25 mM	0.1622 mL	0.8111 mL	1.6221 mL	4.0553 mL
	30 mM	0.1352 mL	0.6759 mL	1.3518 mL	3.3794 mL
	40 mM	0.1014 mL	0.5069 mL	1.0138 mL	2.5346 mL
	50 mM	0.0811 mL	0.4055 mL	0.8111 mL	2.0277 mL
	60 mM	0.0676 mL	0.3379 mL	0.6759 mL	1.6897 mL
	80 mM	0.0507 mL	0.2535 mL	0.5069 mL	1.2673 mL
	100 mM	0.0406 mL	0.2028 mL	0.4055 mL	1.0138 mL