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  3. NADA-green

NADA-green  (Synonyms: NADA hydrochloride)

Cat. No.: HY-D1117 Purity: ≥95.0%
COA Handling Instructions

NADA-green is a fluorescent D-amino acid probe. NADA-green is efficiently incorporated into the peptidoglycan of diverse bacterial species peptidoglycan biosynthesis. NADA-green allows probing of bacterial growth with minimal perturbation.

For research use only. We do not sell to patients.

NADA-green Chemical Structure

NADA-green Chemical Structure

CAS No. : 2253733-11-6

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Based on 1 publication(s) in Google Scholar

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Description

NADA-green is a fluorescent D-amino acid probe. NADA-green is efficiently incorporated into the peptidoglycan of diverse bacterial species peptidoglycan biosynthesis. NADA-green allows probing of bacterial growth with minimal perturbation[1].

In Vitro

Excitation maximum~ 450 nm; emission maximum~ 555 nm[1].
D-amino acids are known to modify and strengthen the peptidoglycan layer. Fluorescent D-amino acids (FDAAs) are efficiently incorporated into the peptidoglycan of diverse bacterial species at the sites of active peptidoglycan biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

303.66

Formula

C9H10ClN5O5

CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

555

Excitation (Ex)

450

SMILES

N[C@H](CNC1=CC=C([N+]([O-])=O)C2=NON=C21)C(O)=O.[H]Cl

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 30 mg/mL (98.79 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 3.2932 mL 16.4658 mL 32.9316 mL
5 mM 0.6586 mL 3.2932 mL 6.5863 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 3.2932 mL 16.4658 mL 32.9316 mL 82.3289 mL
5 mM 0.6586 mL 3.2932 mL 6.5863 mL 16.4658 mL
10 mM 0.3293 mL 1.6466 mL 3.2932 mL 8.2329 mL
15 mM 0.2195 mL 1.0977 mL 2.1954 mL 5.4886 mL
20 mM 0.1647 mL 0.8233 mL 1.6466 mL 4.1164 mL
25 mM 0.1317 mL 0.6586 mL 1.3173 mL 3.2932 mL
30 mM 0.1098 mL 0.5489 mL 1.0977 mL 2.7443 mL
40 mM 0.0823 mL 0.4116 mL 0.8233 mL 2.0582 mL
50 mM 0.0659 mL 0.3293 mL 0.6586 mL 1.6466 mL
60 mM 0.0549 mL 0.2744 mL 0.5489 mL 1.3721 mL
80 mM 0.0412 mL 0.2058 mL 0.4116 mL 1.0291 mL
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NADA-green Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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