NADA-green
Based on 1 Customer Validation
NADA-green is a fluorescent D-amino acid probe. NADA-green is efficiently incorporated into the peptidoglycan of diverse bacterial species peptidoglycan biosynthesis. NADA-green allows probing of bacterial growth with minimal perturbation.
For research use only. We do not sell to patients.
- Purity: 95.39%
- CAS No.: 2253733-11-6
- Formula: C9H10ClN5O5
- Molecular Weight:303.66
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Biological Activity
Excitation maximum~ 450 nm; emission maximum~ 555 nm[1].
D-amino acids are known to modify and strengthen the peptidoglycan layer. Fluorescent D-amino acids (FDAAs) are efficiently incorporated into the peptidoglycan of diverse bacterial species at the sites of active peptidoglycan biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
555
450
Chemical Information
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CAS No. 2253733-11-6
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Appearance Solid
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Molecular Weight 303.66
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Formula C9H10ClN5O5
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Color Brown to reddish brown
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SMILES
N[C@H](CNC1=CC=C([N+]([O-])=O)C2=NON=C21)C(O)=O.[H]Cl
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Synonyms
NADA hydrochloride
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (329.32 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.2932 mL | 16.4658 mL | 32.9316 mL | 82.3289 mL |
| 5 mM | 0.6586 mL | 3.2932 mL | 6.5863 mL | 16.4658 mL | |
| 10 mM | 0.3293 mL | 1.6466 mL | 3.2932 mL | 8.2329 mL | |
| 15 mM | 0.2195 mL | 1.0977 mL | 2.1954 mL | 5.4886 mL | |
| 20 mM | 0.1647 mL | 0.8233 mL | 1.6466 mL | 4.1164 mL | |
| 25 mM | 0.1317 mL | 0.6586 mL | 1.3173 mL | 3.2932 mL | |
| 30 mM | 0.1098 mL | 0.5489 mL | 1.0977 mL | 2.7443 mL | |
| 40 mM | 0.0823 mL | 0.4116 mL | 0.8233 mL | 2.0582 mL | |
| 50 mM | 0.0659 mL | 0.3293 mL | 0.6586 mL | 1.6466 mL | |
| 60 mM | 0.0549 mL | 0.2744 mL | 0.5489 mL | 1.3721 mL | |
| 80 mM | 0.0412 mL | 0.2058 mL | 0.4116 mL | 1.0291 mL | |
| 100 mM | 0.0329 mL | 0.1647 mL | 0.3293 mL | 0.8233 mL |