ER-Tracker Blue-White DPX

Guide (The following is our recommended protocol. This protocol only provides guidance and should be modified according to your specific needs).
1. Preparation of ER-Tracker working solution
1.1 Preparation of stock solution
Dissolve 100 μg ER-Tracker in 172 μL anhydrous DMSO to prepare a 1 mM stock solution.
1.2 Preparation of working solution
It is recommended to use Hank's balanced salt solution (HBSS/Ca/Mg) containing Ca²⁺ and Mg²⁺ to dilute the stock solution and prepare an ER-Tracker working solution of 100 nM-1 μM.
Note: Please adjust the concentration of the ER-Tracker working solution according to actual conditions and prepare fresh before use.
2. Cell staining (suspension cells)
2.1 Collect cells by centrifugation, wash twice with PBS for 5 minutes each time. Adjust the cell density to 1×10⁶/mL.
2.2 Add 1 mL ER-Tracker working solution and incubate at room temperature for 5-30 minutes.
2.3 Centrifuge at 400 g for 3-4 minutes and discard the supernatant.
2.4 Wash the cells twice with PBS for 5 minutes each time.
2.5 Resuspend the cells in 1 mL serum-free medium or PBS, then observe using a fluorescence microscope or flow cytometer.
3. Cell staining (adherent cells)
3.1 Culture adherent cells on sterile coverslips.
3.2 Remove the coverslips from the culture medium and aspirate excess medium.
3.3 Add 100 μL staining working solution, gently shake to ensure complete coverage of the cells, and incubate for 5-30 minutes.
3.4 Remove the staining working solution and wash with culture medium 2-3 times for 5 minutes each time, then observe using a fluorescence microscope.
Note: If flow cytometry analysis is required, digest the cells with trypsin, resuspend, and then proceed with staining.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.