Phenazine ethosulfate
Based on 1 Customer Validation
Phenazine ethosulfate is a cationic dye (Ex=390 nm, Em=530 nm) and electron acceptor that can be used in dye-linked enzyme assays. Phenazine ethosulfate exhibits weak, pH-dependent fluorescence properties and serves as an oxidative substrate. Phenazine ethosulfate promotes the reduction of equine heart metmyoglobin to ferrohemoglobin. As an intermediate, Phenazine ethosulfate can be used in combination with ascorbic acid for the detection of nitric oxide reductase activity.
For research use only. We do not sell to patients.
- Purity: 99.85%
- CAS No.: 10510-77-7
- Formula: C16H18N2O4S
- Molecular Weight:334.39
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Phenazine ethosulfate exists only in cationic form in buffered aqueous solutions with a pH range of 0-12, and its absorption spectrum does not change with pH. Its maximum absorption peaks are located at 387.5 nm, 440 nm, and 490 nm[2].
Phenazine ethosulfate (5.5 × 10-5 M; room temperature) exhibits weak pH-dependent fluorescence in aqueous buffer solutions: no fluorescence is observed under acidic conditions, weak fluorescence (524 nm) appears at neutral pH, and redshifted and enhanced fluorescence (530 nm) occurs under alkaline conditions[2].
Phenazine ethosulfate (5 μM) reduces equine heart metmyoglobin to ferrohemoglobin[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
530
390
Chemical Information
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CAS No. 10510-77-7
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Appearance Solid
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Molecular Weight 334.39
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Formula C16H18N2O4S
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Color Yellow to orange
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SMILES
O=S([O-])(OCC)=O.CC[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 100 mg/mL (299.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. Ghosh R, et al. Phenazine ethosulfate as a preferred electron acceptor to phenazine methosulfate in dye-linked enzyme assays. Anal Biochem. 1979 Oct 15;99(1):112-7. [Content Brief]
[2]. Ryazanova OA, et al. pH-induced changes in electronic absorption and fluorescence spectra of phenazine derivatives. Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy. 2007 Apr;66(4-5):849-59. [Content Brief]
[3]. van Wonderen JH, et al. The nitric oxide reductase activity of cytochrome c nitrite reductase from Escherichia coli. The Journal of biological chemistry. 2008 Apr 11;283(15):9587-94. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9905 mL | 14.9526 mL | 29.9052 mL | 74.7630 mL |
| 5 mM | 0.5981 mL | 2.9905 mL | 5.9810 mL | 14.9526 mL | |
| 10 mM | 0.2991 mL | 1.4953 mL | 2.9905 mL | 7.4763 mL | |
| 15 mM | 0.1994 mL | 0.9968 mL | 1.9937 mL | 4.9842 mL | |
| 20 mM | 0.1495 mL | 0.7476 mL | 1.4953 mL | 3.7381 mL | |
| 25 mM | 0.1196 mL | 0.5981 mL | 1.1962 mL | 2.9905 mL | |
| 30 mM | 0.0997 mL | 0.4984 mL | 0.9968 mL | 2.4921 mL | |
| 40 mM | 0.0748 mL | 0.3738 mL | 0.7476 mL | 1.8691 mL | |
| 50 mM | 0.0598 mL | 0.2991 mL | 0.5981 mL | 1.4953 mL | |
| 60 mM | 0.0498 mL | 0.2492 mL | 0.4984 mL | 1.2460 mL | |
| 80 mM | 0.0374 mL | 0.1869 mL | 0.3738 mL | 0.9345 mL | |
| 100 mM | 0.0299 mL | 0.1495 mL | 0.2991 mL | 0.7476 mL |
Phenazine ethosulfate Related Classifications
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