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  3. Fluorescent Dye
  4. Phenazine ethosulfate

Phenazine ethosulfate is a cationic dye (Ex=390 nm, Em=530 nm) and an electron acceptor that can be used in dye-linked enzyme assays. Phenazine ethosulfate is an intermediate for detecting nitric oxide reducatase (Nors) activity with the presence of ascorbic acid.

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Phenazine ethosulfate Chemical Structure

Phenazine ethosulfate Chemical Structure

CAS No. : 10510-77-7

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Phenazine ethosulfate Related Antibodies

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Description

Phenazine ethosulfate is a cationic dye (Ex=390 nm, Em=530 nm) and an electron acceptor that can be used in dye-linked enzyme assays. Phenazine ethosulfate is an intermediate for detecting nitric oxide reducatase (Nors) activity with the presence of ascorbic acid[1][2][3].

Molecular Weight

334.39

Formula

C16H18N2O4S
CAS No.
Appearance

Solid

Color

Yellow to orange

Emission (Em)

530
Excitation (Ex)

390
SMILES

O=S([O-])(OCC)=O.CC[N+]1=C2C=CC=CC2=NC3=C1C=CC=C3
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (299.05 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.9905 mL 14.9526 mL 29.9052 mL
5 mM 0.5981 mL 2.9905 mL 5.9810 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.9905 mL 14.9526 mL 29.9052 mL 74.7630 mL
5 mM 0.5981 mL 2.9905 mL 5.9810 mL 14.9526 mL
10 mM 0.2991 mL 1.4953 mL 2.9905 mL 7.4763 mL
15 mM 0.1994 mL 0.9968 mL 1.9937 mL 4.9842 mL
20 mM 0.1495 mL 0.7476 mL 1.4953 mL 3.7381 mL
25 mM 0.1196 mL 0.5981 mL 1.1962 mL 2.9905 mL
30 mM 0.0997 mL 0.4984 mL 0.9968 mL 2.4921 mL
40 mM 0.0748 mL 0.3738 mL 0.7476 mL 1.8691 mL
50 mM 0.0598 mL 0.2991 mL 0.5981 mL 1.4953 mL
60 mM 0.0498 mL 0.2492 mL 0.4984 mL 1.2460 mL
80 mM 0.0374 mL 0.1869 mL 0.3738 mL 0.9345 mL
100 mM 0.0299 mL 0.1495 mL 0.2991 mL 0.7476 mL
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Phenazine ethosulfate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Phenazine ethosulfate10510-77-7Fluorescent DyeCationic dyeelectron acceptorNorsNOfluorescent dyeprobeInhibitorinhibitorinhibit

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