N0S Dyes

DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.

2,3-Diaminonaphthalene is a fluorescent reagent used to detect selenium and nitric oxide (NO). 2,3-Diaminonaphthalene binds to selenium to form pyrazolene selenol, with excitation/emission maxima at 365 nm and 525 nm, respectively. 2,3-Diaminonaphthalene binds to NO2− to form the fluorescent product, 1-(H)-napthotriazole, with excitation/emission maxima at 360 nm and 440 nm, respectively.

HKGreen-4I is a highly sensitive green fluorescent probe for the specific detection of ONOO^- in living cells with a maximum excitation wavelength and emission wavelength of 520 nm and 543 nm, respectively.

Phenazine ethosulfate is a cationic dye (Ex=390 nm, Em=530 nm) and an electron acceptor that can be used in dye-linked enzyme assays. Phenazine ethosulfate is an intermediate for detecting nitric oxide reducatase (Nors) activity with the presence of ascorbic acid.

4,5-Diaminofluorescein is a fluorescent detector for nitric oxide (NO) in cells and tissues.

HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.

1,2-Diaminoanthraquinone is a sensitivity, specificity and nontoxic nitric oxide (NO) fluorescent probe. 1,2-Diaminoanthraquinone can be used to detect NO productions in live cell and animals with a maximum of absorption at about 540 nm and a detection limit of 5 μM for NO.

RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm).

2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pK_a ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles. 2',7'-Difluorofluorescein undergoes buffer-mediated and buffer-free excited-state proton transfer between different protonated forms, and its cationic form undergoes rapid excited-state deprotonation. 2',7'-Difluorofluorescein is resistant to photodegradation, maintains stable absorption and fluorescence properties within the physiological pH range, and serves as a fluorescent protein label, a component of Ca²⁺ indicators, a fluorescent imaging agent, and an anisotropy probe.

PYSNO is a lysosome-targeted fluorescent probe based on a pyridazinone skeleton (λ_em=515-565 nm, λ_ex=405 nm) that can be used to track nitric oxide (NO) production in vivo. PYSNO exhibits a rapid, highly sensitive and highly selective "turn-on" response to endogenous and exogenous NO by blocking photoinduced electron transfer and regulating radiative decay rates. PYSNO enables precise in vivo monitoring in a mouse model of myocardial fibrosis and can be applied to the research of related diseases.

NIR-PN1 is a blood-brain barrier-permeable near-infrared fluorescent indicator targeting peroxynitrite anion (ONOO⁻) (Ex/Em = 510 nm/670 nm). NIR-PN1 reacts with ONOO⁻ to trigger a strong near-infrared fluorescence enhancement, enabling the detection of ONOO⁻ flux. NIR-PN1 allows the imaging of ONOO⁻ flux in various Parkinson's disease models. NIR-PN1 is applicable to Parkinson's disease-related research.

HDAC-IN-101 is a HDAC1 inhibitor and nitroreductase/pH-activated fluorescent inducer, with an IC₅₀ of 65 nM against human HDAC1. HDAC-IN-101 blocks cancer cell proliferation by inhibiting HDAC1. HDAC-IN-101 is reduced by overexpressed nitroreductase to generate H6AQ (Ex/Em = 450 nm/500 nm), a product that emits fluorescence under low pH conditions. HDAC-IN-101 is applicable for cancer-related research.

Golgi-NO is a Golgi-targeted fluorescent probe for nitric oxide (NO) (Ex/Em: 560 nm/589 nm). Golgi-NO exhibits excellent selectivity for various potential interfering substances. Golgi-NO can be used to study the function of NO within the Golgi apparatus in disease models such as Alzheimer's disease (AD).