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  4. HKYellow-AM (6/12-mixture)

HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity.

For research use only. We do not sell to patients.

HKYellow-AM (6/12-mixture)

HKYellow-AM (6/12-mixture) Chemical Structure

CAS No. : 1448821-89-3

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Based on 1 publication(s) in Google Scholar

HKYellow-AM (6/12-mixture) Related Antibodies

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Description

HKYellow-AM (6/12-mixture) is a yellow fluorescent probe that can detect ONOO- in living cells and tissues with high selectivity and sensitivity without cytotoxicity[2].

In Vitro

Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Preparation of HKYellow-AM (6/12-mixture) working solution
1.1 Preparation of the stock solution
Dissolve HKYellow-AM (6/12-mixture) in DMSO to obtain 10 mM of stock solution.
1.2 Preparation of HKYellow-AM (6/12-mixture) working solution
Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of HKYellow-AM (6/12-mixture) working solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL
b. Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy or flow cytometry.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

HKYellow-AM (6/12-mixture) Related Antibodies
Molecular Weight

1422.40

Formula

C80H78Cl2N4O16
CAS No.
Appearance

Solid

Color

Green to dark green

Emission (Em)

567
Excitation (Ex)

543
SMILES

O=C1OC(C2=C3)(C4=CC=CC=C41)C5=CC(C(CC(C)6C)C)=C(N6CCCC(OCOC(C)=O)=O)C=C5OC2=CC(N(C(C=C7)=CC=C7O)C)=C3Cl.O=C8OC(C9=CC=CC=C98)(C%10=C(O%11)C=CC(C(CC(C)%12C)C)=C%10N%12CCCC(OCOC(C)=O)=O)C(C%11=C%13)=CC(Cl)=C%13N(C(C=C%14)=CC=C%14O)C
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 100 mg/mL (70.30 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.7030 mL 3.5152 mL 7.0304 mL
5 mM 0.1406 mL 0.7030 mL 1.4061 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

  • Molarity Calculator

  • Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass
=
Concentration
×
Volume
×
Molecular Weight *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start)

C1

×
Volume (start)

V1

=
Concentration (final)

C2

×
Volume (final)

V2

In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

g

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 0.7030 mL 3.5152 mL 7.0304 mL 17.5759 mL
5 mM 0.1406 mL 0.7030 mL 1.4061 mL 3.5152 mL
10 mM 0.0703 mL 0.3515 mL 0.7030 mL 1.7576 mL
15 mM 0.0469 mL 0.2343 mL 0.4687 mL 1.1717 mL
20 mM 0.0352 mL 0.1758 mL 0.3515 mL 0.8788 mL
25 mM 0.0281 mL 0.1406 mL 0.2812 mL 0.7030 mL
30 mM 0.0234 mL 0.1172 mL 0.2343 mL 0.5859 mL
40 mM 0.0176 mL 0.0879 mL 0.1758 mL 0.4394 mL
50 mM 0.0141 mL 0.0703 mL 0.1406 mL 0.3515 mL
60 mM 0.0117 mL 0.0586 mL 0.1172 mL 0.2929 mL
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HKYellow-AM (6/12-mixture) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

HKYellow-AM (6/12-mixture)1448821-89-3Fluorescent DyefluorogenicprobeperoxynitriteInhibitorinhibitorinhibit

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