1,2-Diaminoanthraquinone
Based on 1 Customer Validation
1,2-Diaminoanthraquinone is a sensitivity, specificity and nontoxic nitric oxide (NO) fluorescent probe. 1,2-Diaminoanthraquinone can be used to detect NO productions in live cell and animals with a maximum of absorption at about 540 nm and a detection limit of 5 μM for NO.
For research use only. We do not sell to patients.
- Purity: 91.06%
- CAS No.: 1758-68-5
- Formula: C14H10N2O2
- Molecular Weight:238.25
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Storage:
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
1,2-Diaminoanthraquinone and the product formed upon its reaction with NO, DAA-TZ, can be spectrally resolved using fluorescence spectroscopy and confocal microscopy[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Raw 264.7 macrophage cells
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Concentration:27.4 μM
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Incubation Time:20 min
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Result:Reacted with NO in the presence of oxygen in the intracellular environment to yield DAA-TZ.
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Chemical Information
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CAS No. 1758-68-5
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Appearance Solid
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Molecular Weight 238.25
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Formula C14H10N2O2
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Color Brown to black
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SMILES
O=C1C2=C(C=CC=C2)C(C3=CC=C(N)C(N)=C13)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 62.5 mg/mL (262.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
[1]. María J Marín, et al. Fluorescence of 1,2-diaminoanthraquinone and its nitric oxide reaction product within macrophage cells. Chembiochem. 2011 Nov 4;12(16):2471-7. [Content Brief]
[2]. Francisco Galindo, et al. Spectroscopic studies of 1,2-diaminoanthraquinone (DAQ) as a fluorescent probe for the imaging of nitric oxide in living cells. Photochem Photobiol Sci. 2008 Jan;7(1):126-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.1973 mL | 20.9864 mL | 41.9727 mL | 104.9318 mL |
| 5 mM | 0.8395 mL | 4.1973 mL | 8.3945 mL | 20.9864 mL | |
| 10 mM | 0.4197 mL | 2.0986 mL | 4.1973 mL | 10.4932 mL | |
| 15 mM | 0.2798 mL | 1.3991 mL | 2.7982 mL | 6.9955 mL | |
| 20 mM | 0.2099 mL | 1.0493 mL | 2.0986 mL | 5.2466 mL | |
| 25 mM | 0.1679 mL | 0.8395 mL | 1.6789 mL | 4.1973 mL | |
| 30 mM | 0.1399 mL | 0.6995 mL | 1.3991 mL | 3.4977 mL | |
| 40 mM | 0.1049 mL | 0.5247 mL | 1.0493 mL | 2.6233 mL | |
| 50 mM | 0.0839 mL | 0.4197 mL | 0.8395 mL | 2.0986 mL | |
| 60 mM | 0.0700 mL | 0.3498 mL | 0.6995 mL | 1.7489 mL | |
| 80 mM | 0.0525 mL | 0.2623 mL | 0.5247 mL | 1.3116 mL | |
| 100 mM | 0.0420 mL | 0.2099 mL | 0.4197 mL | 1.0493 mL |
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