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  4. 2',7'-Difluorofluorescein

2',7'-Difluorofluorescein  (Synonyms: Oregon green 488)

Cat. No.: HY-D0077 Purity: 99.52%
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2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pKa ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles. 2',7'-Difluorofluorescein undergoes buffer-mediated and buffer-free excited-state proton transfer between different protonated forms, and its cationic form undergoes rapid excited-state deprotonation. 2',7'-Difluorofluorescein is resistant to photodegradation, maintains stable absorption and fluorescence properties within the physiological pH range, and serves as a fluorescent protein label, a component of Ca2+ indicators, a fluorescent imaging agent, and an anisotropy probe.

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2',7'-Difluorofluorescein

2',7'-Difluorofluorescein Chemical Structure

CAS No. : 195136-58-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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10 mM * 1 mL in DMSO In-stock
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2',7'-Difluorofluorescein Related Antibodies

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Description

2',7'-Difluorofluorescein (Oregon green 488) is a fluorescein derivative and a pH-sensitive fluorescent probe (pKa ~4.7). Upon excitation at 488 nm, 2',7'-Difluorofluorescein exhibits pH-sensitive fluorescence intensity through the formation of dianions, while its pH sensitivity decreases under excitation at 450 nm, allowing its use in ratiometric pH analysis. 2',7'-Difluorofluorescein can be used for the quantitative analysis of pH values in the range of 2-7 in submicron aerosol particles. 2',7'-Difluorofluorescein undergoes buffer-mediated and buffer-free excited-state proton transfer between different protonated forms, and its cationic form undergoes rapid excited-state deprotonation. 2',7'-Difluorofluorescein is resistant to photodegradation, maintains stable absorption and fluorescence properties within the physiological pH range, and serves as a fluorescent protein label, a component of Ca2+ indicators, a fluorescent imaging agent, and an anisotropy probe[1][2][3].

In Vitro

2',7'-Difluorofluorescein enables ratiometric pH measurement of bulk aqueous solutions via AFC, with a pH-responsive fluorescence intensity ratio (I488/I450)ₙₒᵣₘ, a pKa of ~4.7, and no significant ionic strength dependence[1].
2',7'-Difluorofluorescein (10-4 mass fraction; 5 s shutter interval) enables ratiometric pH measurement of sub-micrometer atmospheric aerosol particles via AFC, covering an acidic pH range of 2.38-5.35 with reasonable agreement to indicator paper measurements for most particle types[1].
2',7'-Difluorofluorescein (5.0 μM) in aqueous solution exhibits pH-dependent steady-state fluorescence, with the cation acting as a super-photoacid and an excited-state neutral/monoanion pKa of 2.67[2].
2',7'-Difluorofluorescein (2.5 μM; 5 μM-1.5 M buffer concentrations) in aqueous solution with HOAc/OAc- buffer promotes excited-state proton transfer between the monoanion and dianion forms, with an apparent excited-state pKa of 4.42[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

2',7'-Difluorofluorescein Related Antibodies
Molecular Weight

368.29

Formula

C20H10F2O5
CAS No.
Appearance

Solid

Color

Light brown to brown

Emission (Em)

515
Excitation (Ex)

495
SMILES

O=C1OC2(C3=C(OC4=C2C=C(F)C(O)=C4)C=C(O)C(F)=C3)C5=C1C=CC=C5
Shipping

Room temperature in continental US; may vary elsewhere.
Storage
Powder -20°C 3 years
4°C 2 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (135.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.7153 mL 13.5763 mL 27.1525 mL
5 mM 0.5431 mL 2.7153 mL 5.4305 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 2.7153 mL 13.5763 mL 27.1525 mL 67.8813 mL
5 mM 0.5431 mL 2.7153 mL 5.4305 mL 13.5763 mL
10 mM 0.2715 mL 1.3576 mL 2.7153 mL 6.7881 mL
15 mM 0.1810 mL 0.9051 mL 1.8102 mL 4.5254 mL
20 mM 0.1358 mL 0.6788 mL 1.3576 mL 3.3941 mL
25 mM 0.1086 mL 0.5431 mL 1.0861 mL 2.7153 mL
30 mM 0.0905 mL 0.4525 mL 0.9051 mL 2.2627 mL
40 mM 0.0679 mL 0.3394 mL 0.6788 mL 1.6970 mL
50 mM 0.0543 mL 0.2715 mL 0.5431 mL 1.3576 mL
60 mM 0.0453 mL 0.2263 mL 0.4525 mL 1.1314 mL
80 mM 0.0339 mL 0.1697 mL 0.3394 mL 0.8485 mL
100 mM 0.0272 mL 0.1358 mL 0.2715 mL 0.6788 mL
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2',7'-Difluorofluorescein Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

2',7'-Difluorofluorescein195136-58-4Oregon green 488Oregon green488Oregon green-488Fluorescent DyepH-sensitive fluorescent probephotodegradationglycerol kinase variantsexcited-state proton transferCa2+ indicator componentanisotropy probefluorescence imaging agentextrinsic fluorophoresub-micrometer aerosol particlesfluorescent protein labelInhibitorinhibitorinhibit

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