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  4. RB-OPD

RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm).

For research use only. We do not sell to patients.

RB-OPD

RB-OPD Chemical Structure

CAS No. : 1034863-51-8

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 1 publication(s) in Google Scholar

RB-OPD Related Antibodies

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Description

RB-OPD (NO-red) is a o-phenylenediamine (OPD)-locked rhodamine nitric oxide (NO) fluorescent probe with great sensitivity and selectivity (λex=550 nm, λem=590 nm)[1].

In Vitro

RB-OPD (compound 1) exhibits "turn-on" type fluorogenic and chromogenic behavior toward NO in aqueous solution with great sensitivity and selectivity, and it can be used for the sensing of NO under normal physiological conditions. NO-red is composed of two moieties: rhodamine B spirolactam as the potential strong fluorophore and chromophore, and o-phenylenediamine as an NO-reactive group in a lactam form as a "masked" NO-sensitive modulator[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RB-OPD Related Antibodies
Molecular Weight

532.68

Formula

C34H36N4O2
CAS No.
Appearance

Solid

Color

Off-white to light yellow

Emission (Em)

590
Excitation (Ex)

550
SMILES

O=C1C2=C(C3(N1C4=C(N)C=CC=C4)C5=CC=C(N(CC)CC)C=C5OC6=CC(N(CC)CC)=CC=C63)C=CC=C2
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 25 mg/mL (46.93 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.8773 mL 9.3865 mL 18.7730 mL
5 mM 0.3755 mL 1.8773 mL 3.7546 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
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Calculation results:
Working solution concentration: mg/mL
Purity & Documentation

Purity: 99.24%

SDS (252 KB)

COA (258 KB) HNMR (287 KB) LCMS (98 KB)

Handling Instructions (2659 KB)
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.8773 mL 9.3865 mL 18.7730 mL 46.9325 mL
5 mM 0.3755 mL 1.8773 mL 3.7546 mL 9.3865 mL
10 mM 0.1877 mL 0.9386 mL 1.8773 mL 4.6932 mL
15 mM 0.1252 mL 0.6258 mL 1.2515 mL 3.1288 mL
20 mM 0.0939 mL 0.4693 mL 0.9386 mL 2.3466 mL
25 mM 0.0751 mL 0.3755 mL 0.7509 mL 1.8773 mL
30 mM 0.0626 mL 0.3129 mL 0.6258 mL 1.5644 mL
40 mM 0.0469 mL 0.2347 mL 0.4693 mL 1.1733 mL
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RB-OPD Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

RB-OPD1034863-51-8NO-redFluorescent DyeNOchromogenicfluorescentrhodamineredo-phenylenediamineInhibitorinhibitorinhibit

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