2,3-Diaminonaphthalene
Based on 1 Customer Validation
2,3-Diaminonaphthalene is a fluorescent reagent used to detect selenium and nitric oxide (NO). 2,3-Diaminonaphthalene binds to selenium to form pyrazolene selenol, with excitation/emission maxima at 365 nm and 525 nm, respectively. 2,3-Diaminonaphthalene binds to NO2− to form the fluorescent product, 1-(H)-napthotriazole, with excitation/emission maxima at 360 nm and 440 nm, respectively.
For research use only. We do not sell to patients.
- Purity: 98.12%
- CAS No.: 771-97-1
- Formula: C10H10N2
- Molecular Weight:158.20
-
Storage:
RT, protect from light.
In solvent -80°C, 1 year , -20°C, 6 months
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Protocol
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
415
365
Chemical Information
-
CAS No. 771-97-1
-
Appearance Solid
-
Molecular Weight 158.20
-
Formula C10H10N2
-
Color Light yellow to brown
-
SMILES
NC1=C(N)C=C2C=CC=CC2=C1
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
RT, protect from light
In solvent -80°C 1 year -20°C 6 months
Solvent & Solubility
DMSO : 100 mg/mL (632.11 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocol
Various concentrations of each drug are incubated for 60 min at 37°C in OS-mL reaction volumes containing PBS (pH 7.4), 200 μM 2,3-Diaminonaphthalene (DAN) and 40 μM Sp/NO. Spermine/NO will spontaneously decompose at pH7.4 to produce 2 mol of NO and 1 mol of spermine with a half-life of 39 min at 37°C. Following the incubation period, 2.5 mL of 10 mM NaOH is added to each tube to stop the reaction. The NO-dependent N-nitrosation of 2,3-Diaminonaphthalene to yield its highly fluorescent N-nitrosated derivative, 2,3-naphthotriazole, is quantified by measuring the fluorescence of each sample using an excitation wavelength of 375 nm and an emission wavelength of 450nm. The concentration of Triazole is determined using standards of the pure 2,3-naphthotriazole[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (557 KB)
- English - EN (557 KB)
- Français - FR (557 KB)
- Deutsch - DE (557 KB)
- Norwegian - NO (557 KB)
- Español - ES (557 KB)
- Swedish - SV (557 KB)
- Italian - IT (557 KB)
- Korean - KR (557 KB)
- Portuguese - PT (557 KB)
-
Handling Instructions (2659 KB)
References
[1]. Tian J, et al. Poly(2,3-diaminonaphthalene) microspheres as a novel quencher for fluorescence-enhanced nucleic acid detection. Analyst. 2011 Jun 7;136(11):2221-4. [Content Brief]
[2]. Martínez-Tomé MJ, et al. Immobilization and characterization of 2,3-diaminonaphthalene/cyclodextrin complexes in a sol-gel matrix: a new fluorimetric sensor for nitrite. J Fluoresc. 2009 Jan;19(1):119-25. [Content Brief]
[3]. Grisham MB, et al. Effects of aminosalicylates and immunosuppressive agents on nitric oxide-dependent N-nitrosation reactions. Biochem Pharmacol. 1994 May 18;47(10):1897-902. [Content Brief]
[4]. Kleinhenz DJ, et al. Detection of endothelial nitric oxide release with the 2,3-diaminonapthalene assay. Free Radic Biol Med. 2003 Apr 1;34(7):856-61. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 6.3211 mL | 31.6056 mL | 63.2111 mL | 158.0278 mL |
| 5 mM | 1.2642 mL | 6.3211 mL | 12.6422 mL | 31.6056 mL | |
| 10 mM | 0.6321 mL | 3.1606 mL | 6.3211 mL | 15.8028 mL | |
| 15 mM | 0.4214 mL | 2.1070 mL | 4.2141 mL | 10.5352 mL | |
| 20 mM | 0.3161 mL | 1.5803 mL | 3.1606 mL | 7.9014 mL | |
| 25 mM | 0.2528 mL | 1.2642 mL | 2.5284 mL | 6.3211 mL | |
| 30 mM | 0.2107 mL | 1.0535 mL | 2.1070 mL | 5.2676 mL | |
| 40 mM | 0.1580 mL | 0.7901 mL | 1.5803 mL | 3.9507 mL | |
| 50 mM | 0.1264 mL | 0.6321 mL | 1.2642 mL | 3.1606 mL | |
| 60 mM | 0.1054 mL | 0.5268 mL | 1.0535 mL | 2.6338 mL | |
| 80 mM | 0.0790 mL | 0.3951 mL | 0.7901 mL | 1.9753 mL | |
| 100 mM | 0.0632 mL | 0.3161 mL | 0.6321 mL | 1.5803 mL |
2,3-Diaminonaphthalene Related Classifications
HY-D0073 Related Classifications
Powered by Bioz