Other Indicator Probes

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu⁺ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu⁺) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.

Bathocuproine disulfonate disodium (BCS) is an organic compound used as a highly sensitive colorimetric reagent for copper ions in biochemical and analytical applications. It has a bright yellow color and absorbs light at specific wavelengths, so it can be used to detect and quantify trace copper. In biochemical applications, BCS is commonly used to study the role of copper ions in various biological processes. Copper is an essential nutrient for many organisms, but it can also be toxic in high concentrations, so accurate measurement of copper levels is important to understand its impact on living systems. In terms of analysis, BCS is often used in environmental monitoring and water quality testing to detect copper pollution. It can detect copper even at very low concentrations, making it an invaluable tool for identifying potential sources of pollution and assessing the impact of industrial activities on aquatic ecosystems.

Copper Fluor-4 (CF4) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a K_d value of 2.9×10⁻¹³ M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).

2,3-Diaminonaphthalene is a fluorescent reagent used to detect selenium and nitric oxide (NO). 2,3-Diaminonaphthalene binds to selenium to form pyrazolene selenol, with excitation/emission maxima at 365 nm and 525 nm, respectively. 2,3-Diaminonaphthalene binds to NO2− to form the fluorescent product, 1-(H)-napthotriazole, with excitation/emission maxima at 360 nm and 440 nm, respectively.

Lumogallion is a highly sensitive fluorescent reagent for the detection of aluminum, gallium and other metals. Lumogallion has an excitation wavelength of 490 nm and an emission spectrum in the range of 520 nm to 650 nm, with a peak near 580 nm.

Azomethine-H monosodium is a colour-forming reagent. Azomethine-H monosodium is also a reagent for boron determinations.

Copper Fluor-4 (solution) (CF4 (solution)) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 solution has high copper selectivity with a K_d value of 2.9×10^?13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 solution is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).
Solvent and concentration: DMSO: 5 mM

DCM-KPV is a fluorescent probe targeting the human intestinal oligopeptide transporter PEPT1/SLC15A1 receptor (λ_ex=480 nm, λ_em=620-670 nm). DCM-KPV specifically binds to PepT1 via its KPV domain and mediates receptor-targeted internalization, thus effectively accumulating in the cytoplasm and nucleus of cells overexpressing this receptor. DCM-KPV has the advantages of long emission wavelength, high emission efficiency, low photobleaching, and negligible cytotoxicity. DCM-KPV maintains stable fluorescence intensity under continuous illumination, exhibiting extremely high live cell compatibility. DCM-KPV can specifically accumulate at colonic inflammatory sites through the intestinal mucosa, enabling direct non-invasive visual differentiation between chronic and acute ulcerative colitis groups and the normal group.

Bathophenanthrolinedisulfonic acid disodium is an iron ion chelator that serves as a colorimetric reagent for iron ion detection. Bathophenanthrolinedisulfonic acid disodium forms stable, strong complexes with Fe²⁺ and Fe³⁺, and each complex exhibits reversible redox behavior. Bathophenanthrolinedisulfonic acid disodium can act as a ligand to form water-soluble ruthenium catalysts, which promote the hydrogenation of the ketone group in levulinic acid to produce γ-valerolactone. When Bathophenanthrolinedisulfonic acid disodium and its iron complex are incorporated into a polymer-modified electrode, rapid color change from red to transparent occurs during potential cycling.

Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator.

CAY10731 (compound 3) is a highly selective fluorescent probe for detection of hydrogen sulfide (H₂S). CAY10731 is used to monitor exo- and endogenous H₂S in both cancer and normal cells. CAY10731 is applied for imaging of H₂S in living tissues at variable depths and in nematodes.

BD-Oligo is an oligomer-specific fluorescent chemical probe. BD-Oligo preferentially identifies Aβ oligomer assemblies over monomers or fibrils by using diversity-directed fluorescent library (DOFL) screening and computational techniques. BD-Oligo exhibits dynamic oligomer monitoring capabilities during Aβ peptide fibril formation as Aβ is induced to form oligomers and ultimately fibrils over time. BD-Oligo also exhibits blood-brain barrier permeability with the ability to stain Aβ oligomers in vivo.

PS-SCOTfluor-12 (compound 10) is a fluorescent probe for imaging Lactic acid (HY-B2227) metabolism in live cells. λex/λem= 485/605 nm.

Arsenazo III is an azo derivative of chromotropic acid, a metal chrome dye, a chelating agent, and a cation complexing agent. Arsenazo III forms stable 1:1 complexes with Ca²⁺, Mg²⁺, Sr²⁺, Ba²⁺, K⁺, and Na⁺, and its binding affinity for Ca²⁺ depends on pH, alkali metal cation concentration, and buffer parameters. Arsenazo III serves as a colorimetric indicator for micromolar ionized Ca²⁺ in cells.

Sel-green, a selective selenol fluorescent probe, is applied to quantify the Sec content in the selenoenzyme thioredoxin reductase and image endogenous Sec in live HepG2 cells.

10-(4-(Bis(2-acetoxyethyl)amino)phenyl)-BODIPY 505/515 is a fluorescent dye for Cu²⁺ ion.

BTD probe-1 is a benzothiazine-based chemoproteomic probe and selective protein S-sulfenic acid (Cys-SOH) labeling agent. BTD probe-1 labels protein S-sulfenic acids in vitro in cell and tissue samples, and in situ in intact cells, enabling detection or enrichment of modified proteins/peptides. BTD probe-1 exhibits no cytotoxicity in cells at concentrations ≤1 mM. BTD probe-1 enables global, site-specific mapping and quantification of cysteine S-sulfenylation in complex proteomes with lower input material.

Cobalt(II) ions probe 1 (Compound L) is a fluorescent sensor for Co²⁺ detecting in biological environments. Cobalt(II) ions probe 1 can selectively bind with Co²⁺ in the presence of other metal ions (Ex: 380 nm).

Bromothymol Blue sodium salt is a pH indicator. Storage: protect from light.

Bromopyrogallol red (Dibromopyrogallolsulfonphthaleine) is a multifunctional dye. Dyes are important tools in biological experiments. They can help researchers observe and analyze cell structures, track biomolecules, evaluate cell functions, distinguish cell types, detect biomolecules, study tissue pathology and monitor microorganisms. Their applications range from basic scientific research to clinical A wide range of diagnostics. Dyes are also widely used in traditional fields such as textile dyeing, as well as in emerging fields such as functional textile processing, food pigments and dye-sensitized solar cells.