Ion Fluorescent Probes

Fluo-4 AM is a cell-permeable Ca²⁺ (calcium ion) indicator (Ex/Em = 485/526 nm).

BODIPY 581/591 C11 is a BODIPY borofluoroprene derivative with good light stability and low fluorescence artifacts. BODIPY 581/591 C11 can be used for study lipid peroxidation and antioxidant properties in living cells, or detect ferroptosis by reaction with hydroxyl radicals. BODIPY 581/591 C11 is emitted at 591 nm (reduced prototype), or redshifted to 510 nm (oxidized type). The excitation wavelengths were 581 nm (reduced prototype) and 500 nm (oxidized type).

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu⁺ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu⁺) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.

RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe²⁺. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe²⁺ in living cells, and tends to be localized in the Golgi apparatus.

MQAE is a chloride ion (Cl^-) fluorescent probe that can be used to measure chloride concentrations. The fluorescence intensity of MQAE decreases proportionally as Cl^- ions increase. MQAE has high cell permeability and is suitable for fluorescence detection such as confocal microscopy and flow cytometry (Ex/Em=350/460 nm).

Fluo-3AM (solution) is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.
Solvent and concentration: DMSO: 5 mM

RhoNox-4 is a Fe²⁺-selective fluorescence-activatable probe and an inhibitor of ferroptosis. RhoNox-4 shows no obvious cytotoxicity even at concentrations up to 50 μM. RhoNox-4 can selectively oxidize labile Fe²⁺ to Fe³⁺, depleting the iron pool required for lipid peroxidation and ferroptosis. RhoNox-4 inhibits ferroptosis induced by ferroptosis inducers; its activity depends on localization to the endoplasmic reticulum/Golgi apparatus, and it does not affect apoptosis or necrosis. RhoNox-4 can be used for imaging labile Fe²⁺ in living cells, and also enables high-throughput screening of labile iron homeostasis regulators based on 96-well plates. RhoNox-4 allows visualization of cytoplasmic Fe²⁺ levels in root tips of *Arabidopsis* (Ex/Em = 530-560/573-647 nm).

Copper Fluor-4 (solution) (CF4 (solution)) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 solution has high copper selectivity with a K_d value of 2.9×10^?13 M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 solution is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).
Solvent and concentration: DMSO: 5 mM

TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λ_max ~470 nm). TSQ can combine with Zn²⁺ in the presence of Ca²⁺ and Mg²⁺ to produce blue fluorescence (Ex/Em = 334/495 nm).

Ferrozine is a spectrophotometric reagent for iron ions, can react with divalent Fe to form a stable magenta complex species. The complex has an absorption peak at 562 nm. Ferrozine-based colorimetric assays can quantify iron in cells

Bathocuproine disulfonate disodium (BCS) is an organic compound used as a highly sensitive colorimetric reagent for copper ions in biochemical and analytical applications. It has a bright yellow color and absorbs light at specific wavelengths, so it can be used to detect and quantify trace copper. In biochemical applications, BCS is commonly used to study the role of copper ions in various biological processes. Copper is an essential nutrient for many organisms, but it can also be toxic in high concentrations, so accurate measurement of copper levels is important to understand its impact on living systems. In terms of analysis, BCS is often used in environmental monitoring and water quality testing to detect copper pollution. It can detect copper even at very low concentrations, making it an invaluable tool for identifying potential sources of pollution and assessing the impact of industrial activities on aquatic ecosystems.

Mag-Fluo-4 AM, an analog of Fluo-4 AM (HY-101896), is an intracellular Mg²⁺ (K_d = 4.7 mM) indicator and a Ca²⁺ (K_d = 22 μM) indicator. Mag-Fluo-4 AM suitable for monitoring high concentrations and rapidly changing Ca²⁺ signals, and changes in intracellular Mg²⁺ homeostasis (Ex/Em = 496/525 nm).

ANS (8-Anilino-1-naphthalenesulfonic acid) is a competitive inhibitor targeting thyroxine binding globulin (TBG) (K_i=2.09×10⁶ M^-1). ANS is used in radioimmunoassay by displacing bound triiodothyronine (T3) to improve detection sensitivity. ANS can block the protein binding site of T3 and release free T3 for antibody recognition. As a fluorescent probe, ANS can specifically bind to the hydrophobic region of proteins (such as membrane proteins) and monitor the dynamics of protein conformation through changes in fluorescent signals. It is widely used in biochemical research and antibacterial material development.

Fura-2 AM is a membrane permeable, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ (calcium ion) indicator. Fura-2 AM crosses cell membranes and is converted to Fura-2 (HY-D0110A) via cellular esterases. Fura-2 AM can be used to detect calcium in cells.

Fluo-3 AM is a fluorecent Ca²⁺ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

Copper Fluor-4 (CF4) is a Cu⁺-specific fluorescent probe based on a rhodol dye scaffold. Copper Fluor-4 has high copper selectivity with a K_d value of 2.9×10⁻¹³ M, particularly over zinc and iron, as well as abundant cellular alkali and alkaline earth metals. Copper Fluor-4 is stable in a physiologically relevant pH regime between 6 and 8 (wavelengths of 415 nm for excitation and 660 nm for emission).

Zinquin ethyl ester is a cell-permeable and lipophilic fluorescent derivative of Zinquin (HY-D0982). Zinquin ethyl ester reacts with protein-bound Zn²⁺ in cells and forms fluorescent ternary adducts. Zinquin ethyl ester undergoes hydrolysis by intracellular esterases impeding its efflux across the plasma membrane (Ex/Em = 370/470 nm).

2,3-Diaminonaphthalene is a fluorescent reagent used to detect selenium and nitric oxide (NO). 2,3-Diaminonaphthalene binds to selenium to form pyrazolene selenol, with excitation/emission maxima at 365 nm and 525 nm, respectively. 2,3-Diaminonaphthalene binds to NO2− to form the fluorescent product, 1-(H)-napthotriazole, with excitation/emission maxima at 360 nm and 440 nm, respectively.

Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.

SBFI-AM is a Na⁺ selective fluorescent indicator. SBFI-AM shows selectivity for Na⁺ over K⁺ with K_d values of 6.7 and 166 mM, respectively (Ex = 340/380 nm; Em = 500 nm).