1. Dye Reagents
  2. Fluo-4 AM

Fluo-4 AM 

Cat. No.: HY-101896
Handling Instructions

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).

For research use only. We do not sell to patients.

Fluo-4 AM Chemical Structure

Fluo-4 AM Chemical Structure

CAS No. : 273221-67-3

Size Price Stock Quantity
100 μg USD 432 In-stock
Estimated Time of Arrival: December 31

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Based on 1 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Protocol

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Description

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).

In Vitro

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1].

Storage

-20°C, protect from light, stored under nitrogen

References
Cell Assay
[1]

For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 mM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1096.94

Formula

C₅₁H₅₀F₂N₂O₂₃

CAS No.

273221-67-3

SMILES

O=C(OCOC(C)=O)CN(C1=CC=C(C2=C3C=C(F)C(C=C3OC4=C2C=C(F)C(OCOC(C)=O)=C4)=O)C=C1OCCOC5=CC(C)=CC=C5N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O

Shipping

Room temperature in continental US; may vary elsewhere

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Fluo-4 AM
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Fluo-4 AM

Cat. No.: HY-101896