1. Dye Reagents

Fluo-4 AM 

Cat. No.: HY-101896 Purity: >90.0%
Handling Instructions

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).

For research use only. We do not sell to patients.

Fluo-4 AM Chemical Structure

Fluo-4 AM Chemical Structure

CAS No. : 273221-67-3

Size Price Stock Quantity
100 μg USD 432 In-stock
Estimated Time of Arrival: December 31

* Please select Quantity before adding items.

Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm).

In Vitro

Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9116 mL 4.5581 mL 9.1163 mL
5 mM 0.1823 mL 0.9116 mL 1.8233 mL
10 mM 0.0912 mL 0.4558 mL 0.9116 mL
*Please refer to the solubility information to select the appropriate solvent.
Cell Assay

For measuring fluorescence from cells in suspension, dilutions to 2 to 3×106 cells are made, from cultures of rat basophilic leukemia (RBL) cells. Cells are incubated in suspension in 1 mM dye (including Fluo-4 AM) for 30 min at 37°C. Cell suspensions are then transferred to cuvets for measurements of fluorescence emission intensity by spectrofluorometer[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight









-20°C, protect from light, stored under nitrogen


Room temperature in continental US; may vary elsewhere

Purity: >90.0%

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Fluo-4 AM
Cat. No.:

Fluo-4 AM

Cat. No.: HY-101896