Poloxamer 407 (F127)
Based on 8 publication(s) in Google Scholar
Poloxamer 407 (F127) is a nonionic surfactant that is 100% active and relatively non-toxic to cells at low concentrations, and frequently used with dye AM esters such as Indo-1 AM, Fura-2 AM, Calcein AM, Fluo-3 AM, Fluo-4 AM, Quest Fluo-8 AM and Quest Rhod-4 AM, etc. to improve their water solubility. Poloxamer 407 is also a lipoprotein lipase inhibitor.
For research use only. We do not sell to patients.
- CAS No.: 9003-11-6
- Molecular Weight:12600(Average)
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Poloxamer 407 (F127)
More- Nat Aging. 2026 Feb;6(2):349-367. [Abstract]
- Int J Biol Macromol. 2026 Apr:354:151468. [Abstract]
- Acta Pharmacol Sin. 2025 Nov 1. [Abstract]
- Cell Rep. 2025 Feb 5;44(2):115246. [Abstract]
- FASEB J. 2026 May 31;40(10):e71953. [Abstract]
- Sci Rep. 2025 Jul 1;15(1):21506. [Abstract]
- Dig Dis Sci. 2023 Feb;68(2):478-486. [Abstract]
- Research Square Preprint. 2023 Jul 5.
Customer Validation & Images
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In Vivo Efficacy Study
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In Vivo Efficacy Study
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Histological Imaging/Staining
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Histological Imaging/Staining
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IHC
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Dissolve 1 g of Poloxamer 407 (F127) in 10 mL distilled water to make a 10% (w/v) stock solution, or 2 g of Poloxamer 407 (F127) in 10 mL DMSO to make a 20% (w/v) stock solution. These may require heating from 40 to 50°C for about 30 minutes. Store solution at room temperature. Do not refrigerate or freeze the Poloxamer 407 (F127) solution since it may precipitate. If precipitation is observed, the precipitates can be dissolved by heating to 37°C and vortexing before use.
2. Dilute the 10% or 20% Poloxamer 407 (F127) stock solution into the cell-loading buffer such as Hanks and 20 mM Hepes buffer (HHBS) at 1:1000 to 1:500 dilution to achieve a 0.02 to 0.04% working solution.
3. The DMSO stock solution of AM ester is then diluted into the 0.02 to 0.04% working solution to achieve a final AM ester concentration between 1 μM and 10 μM. The final concentration of Poloxamer 407 (F127) is normally kept at or below 0.08%.
4. The cells are incubated at a desired temperature for between 10 minutes and 1 hour. In general it is desirable to use the minimum amount of AM ester needed to achieve adequate fluorescence signal to noise levels.
5. After labeling, the cells are washed with HHBS or fresh medium before starting the experiment.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Ldlr−/− mice[2]
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Dosage:0.25 g/kg
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Administration:Intraperitoneal injection, every other day for 7 weeks
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Result:Mice tended to have a lower body weight and had smaller epididymal fat pads compared to the saline controls, and had reduced atherosclerosis.
Chemical Information
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CAS No. 9003-11-6
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Appearance Solid
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Molecular Weight 12600(Average)
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Color White to off-white
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SMILES
CC.[H(OCH2CH2)x(OCH2CH)y(OCH2CH2)zOH].[CH3]
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Synonyms
PEG-PPG-PEG, 12600 (Average Mn)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Publications (8)
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Journal Impact Factor
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Most Recent
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Nat Aging
Senotoxins target senescence via lipid binding specificity, ion imbalance and lipidome remodeling. [Abstract]2026 Feb;6(2):349-367. PMID: 41526742 -
Int J Biol Macromol
Lycium barbarum polysaccharides loaded myocardial-decorating nanoplatform for precise treatment of myocardial injury induced by exertional heat stroke. [Abstract]2026 Apr:354:151468. PMID: 41846018 -
Acta Pharmacol Sin
Galangin 3-methyl ether alleviates mouse hypertrophic cardiomyopathy via targeting HDAC2 and subsequently inactivating the PI3K-AKT signaling pathway. [Abstract]2025 Nov 1. PMID: 41176539 -
Cell Rep
Meteorin-like alleviates hepatic steatosis by regulating hepatic triglyceride secretion and fatty acid oxidation. [Abstract]2025 Feb 5;44(2):115246. PMID: 39918960
Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 5;44(2):115246. [Abstract]
We observed a decrease in the serum TG levels after poloxamer 407 (0.2 g/mL, 200 μL, Intrauterine injection) administration as well as a decrease in the serum TG content in LKO-Met mice compared with those in L-WT mice fed an HFD.
Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Cell Rep. 2025 Feb 5;44(2):115246. [Abstract]
We observed a decrease in the serum TG levels after poloxamer 407 (0.2 g/mL, 200 μL, Intrauterine injection) administration as well as a decrease in the hepatic PC content in LKO-Met mice compared with those in L-WT mice fed an HFD.
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FASEB J
Integrated Multi-Omics and Experimental Validation Reveal Dysregulation of the OXPHOS-NADPH-GSH Axis in Renal Fibrosis. [Abstract]2026 May 31;40(10):e71953. PMID: 42175955 -
Sci Rep
S100A8 regulated by estrogen improves injured endometrial epithelium reconstruction by promoting tight junction formation and stromal cell transformation. [Abstract]2025 Jul 1;15(1):21506. PMID: 40596550
Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506. [Abstract]
Distribution of S100A8-positive cells in the endometrium of four different groups with estrogen treatment (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and S100A8 + P407 groups). Black arrows show S100A8-PICs. S100A8-PICs were distributed around the glands and blood vessels in the control group. S100A8-PICs in the site of injury or adhesion in the injury group and in the P407 group. S100A8-PICs reverse migrated to the glands or blood vessels in the S100A8 + P407 group. Scale bar is 100 μm or 1 mm.
Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506. [Abstract]
Ki-67-positive proliferating cells were distributed in the endometrium or uterine interior wall in all groups (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and S100A8 + P407 groups). Scale bar is 100 or 400 μm.
Poloxamer 407 (F127) purchased from MedChemExpress. Usage Cited in: Sci Rep. 2025 Jul 1;15(1):21506. [Abstract]
Immunohistochemical staining showing Claudin-1 distribution in the endometrium of different groups (control, injury, P407 (0.2 g/mL, 200 μL, Intrauterine injection), and P407 + S100A8 groups); black arrows show Claudin-1 accumulated below the cell membrane of the lateral contact zone between adjacent cells and red arrows show cells expressing Claudin-1 in the stroma. Scale bar is 100 μm.
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Dig Dis Sci
Dysregulated B7H4/JAK2/STAT3 Pathway Involves in Hypertriglyceridemia Acute Pancreatitis and Is Attenuated by Baicalin. [Abstract]2023 Feb;68(2):478-486. PMID: 35781653 -
Solvent & Solubility
H2O : 110 mg/mL (Need ultrasonic)
For the following dissolution methods, please prepare the working solution directly:
It is recommended to prepare fresh solutions and use them promptly within a short period of time.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: PBS
Solubility: 50 mg/mL; Clear solution; Need ultrasonic
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Antunes FE, et al. Gels of Pluronic F127 and nonionic surfactants from rheological characterization to controlled drug permeation. Colloids Surf B Biointerfaces. 2011 Oct 1;87(1):42-8. [Content Brief]
[2]. Peng X, et al. Poloxamer 407 Induces Hypertriglyceridemia but Decreases Atherosclerosis in Ldlr-/- Mice. Cells. 2022 May 30;11(11):1795. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Poloxamer 407 (F127) Related Classifications
HY-D1005 Related Classifications
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