1. GPCR/G Protein
    Neuronal Signaling
  2. Cholecystokinin Receptor
  3. Ceruletide

Ceruletide (Synonyms: Caerulein; Cerulein; FI-6934)

Cat. No.: HY-A0190 Purity: 99.96%
Handling Instructions

Ceruletide, a biologically active decapeptide isolated from the skin of the Australian frog Hyla caerulea, is a potent cholecystokinetic agent, and acts as a cholecystokinin receptor agonist.

For research use only. We do not sell to patients.

Ceruletide Chemical Structure

Ceruletide Chemical Structure

CAS No. : 17650-98-5

Size Price Stock Quantity
1 mg USD 160 In-stock
Estimated Time of Arrival: December 31
5 mg USD 550 In-stock
Estimated Time of Arrival: December 31
10 mg USD 950 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 11 publication(s) in Google Scholar

Top Publications Citing Use of Products

    Ceruletide purchased from MCE. Usage Cited in: Am J Transl Res. 2018 Feb 15;10(2):402-410.

    Cerulein exposure increases the protein expression of MD2, while ALR reduces the Cerulein-induced upregulation in MD2 protein expression.

    Ceruletide purchased from MCE. Usage Cited in: Can J Physiol Pharmacol. 2019 Jan;97(1):15-22.

    Expressions of Wnt/β-Catenin pathway-related genes in pancreatic and small intestinal tissues in each group (control, Caerulein, SKL2001+Caerulein, SKL2001+control). Relative mRNA expressions of Wnt1 (A, F), Wnt2 (B, G), β-catenin (C, H), c-Myc (D, I) and Axin-2 (E, J) in pancreatic and small intestinal tissues in each group detected by qRT-PCR.

    Ceruletide purchased from MCE. Usage Cited in: Exp Cell Res. 2019 Sep 4:111606.

    Decreased SPOP expression in cerulein-induced CP mice. Pancreatic SPOP, NF-κB/p65 and downstream proteins are determined through western blotting. Protein levels are normalized to the GAPDH loading control. Densitometry analysis is performed using the ImageJ software.
    • Biological Activity

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    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ceruletide, a biologically active decapeptide isolated from the skin of the Australian frog Hyla caerulea, is a potent cholecystokinetic agent, and acts as a cholecystokinin receptor agonist.

    IC50 & Target

    Cholecystokinin receptor[4]

    In Vitro

    Ceruletide is similar chemically and biologically to the human gastrointestinal hormones cholecystokinin-pancreozymin (CCK) and gastrin II. Ceruletide stimulates gallbladder contraction, pancreatic exocrine secretion, gastric secretion, and motility in the distal duodenum, jejunum, ileum and colon, while delaying gastric emptying and inhibiting motility in the proximal duodenum[1]. Ceruletide in supramaximal but not in physiological doses activates NF-kappaB/Rel in vitro. This activation may induce a self-defending genetic program before the onset of cellular injury, which may prevent higher degrees of damage of pancreatic acinar cells after secretagogue hyperstimulation[2].

    In Vivo

    Ceruletide (0.4-0.5 mcg/kg, i.v.; 3-4 mcg/kg, s.c.) results in emesis and evacuation of the bowel in the intact conscious dog, and recovery is complete 15-30 min after i. v. administration and 2-4 hr after s.c. administration. Ceruletide (5-15 ng/kg, i.v.) shows a marked spasmogenic effect on the pylorus of rats. Ceruletide also reduces blood pressure in anesthetized dogs[1]. Ceruletide serum bile acid (SBA) stimulation circumvents exogenous and endogenous influences associated with postprandial (PP) SBA stimulation. Ceruletide SBA stimulation may perform as well as PP SBA stimulation in dogs with portosystemic shunt (PSS) and be more sensitive for the detection of hepatic dysfunction in dogs with upper respiratory disease (URD)[3].

    Molecular Weight

    1352.41

    Formula

    C₅₈H₇₃N₁₃O₂₁S₂

    CAS No.

    17650-98-5

    Sequence

    {pGlu}-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2

    Sequence Shortening

    {pGlu}-QD-Y(SO3H)-TGWMDF-NH2

    SMILES

    O=C(N[[email protected]@H](CCSC)C(N[[email protected]@H](CC(O)=O)C(N[[email protected]](C(N)=O)CC1=CC=CC=C1)=O)=O)[[email protected]@H](NC(CNC([[email protected]@]([[email protected]](O)C)([H])NC([[email protected]@H](NC([[email protected]](CC(O)=O)NC([[email protected]](CCC(N)=O)NC([[email protected]](CC2)NC2=O)=O)=O)=O)CC3=CC=C(OS(=O)(O)=O)C=C3)=O)=O)=O)CC4=CNC5=C4C=CC=C5

    Shipping

    Room temperature in continental US; may vary elsewhere

    Storage
    Protect from light
    Powder -80°C 2 years
      -20°C 1 year
    In solvent -80°C 6 months
      -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : ≥ 100 mg/mL (73.94 mM)

    DMSO : ≥ 100 mg/mL (73.94 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7394 mL 3.6971 mL 7.3942 mL
    5 mM 0.1479 mL 0.7394 mL 1.4788 mL
    10 mM 0.0739 mL 0.3697 mL 0.7394 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

      Solubility: ≥ 2.08 mg/mL (1.54 mM); Clear solution

    • 2.

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

      Solubility: ≥ 2.08 mg/mL (1.54 mM); Clear solution

    • 3.

      Add each solvent one by one:  10% DMSO    90% corn oil

      Solubility: 2.08 mg/mL (1.54 mM); Clear solution; Need warming

    *All of the co-solvents are provided by MCE.
    References
    Animal Administration
    [3]

    Dogs[3]
    All dogs undergo serum bile acid (SBA) stimulation with food (<5 kg/body weight [BW] 2 teaspoons, >5 kg BW 2 tablespoons) or 0.3 μg/kg BW Ceruletide IM, respectively, on consecutive days. A diet of moderate protein content and with an increased concentration of fiber is chosen to minimize metabolic complications such as hepatic encephalopathy. Before each test, the dogs are fasted for 12 hours. Blood samples are drawn at baseline, 60 and 120 minutes after feeding, and 20, 30, and 40 minutes postinjection, respectively. The blood samples are collected in plain tubes and left to clot; they are then centrifuged at 6,500 ×g for 1 minute, and the serum is used to measure SBA by a colorimetric test with endpoint determination[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References

    Purity: 99.96%

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    Product Name:
    Ceruletide
    Cat. No.:
    HY-A0190
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