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  4. Ceruletide

Ceruletide  (Synonyms: Caerulein; Cerulein; FI-6934)

Cat. No.: HY-A0190 Purity: 99.85%
COA Handling Instructions

Cérulétide est un décapeptide et un récepteur des cholécystokinines puissant. Cérulétide est un agent cholécystocinétique sûr et efficace avec un effet spasmogène direct sur le muscle de la vésicule biliaire et les voies biliaires.

Ceruletid ist ein Decapeptid und ein potenter cholecystokinin receptor-Agonist. Ceruletid ist ein sicheres und wirksames Cholecystokinetikum mit einer direkten spasmogenen Wirkung auf den Gallenblasenmuskel und die Gallengänge.

Ceruletide is a decapeptide and a potent cholecystokinin receptor agonist. Ceruletide is a safe and effective cholecystokinetic agent with a direct spasmogenic effect on the gallbladder muscle and bile ducts.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Ceruletide Chemical Structure

Ceruletide Chemical Structure

CAS No. : 17650-98-5

Size Price Stock Quantity
100 μg USD 40 In-stock
500 μg USD 70 In-stock
1 mg USD 110 In-stock
5 mg USD 420 In-stock
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50 mg   Get quote  

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This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 70 publication(s) in Google Scholar

Top Publications Citing Use of Products

67 Publications Citing Use of MCE Ceruletide

WB
RT-PCR

    Ceruletide purchased from MedChemExpress. Usage Cited in: Exp Cell Res. 2019 Nov 1;384(1):111606.  [Abstract]

    Decreased SPOP expression in cerulein-induced CP mice. Pancreatic SPOP, NF-κB/p65 and downstream proteins are determined through western blotting. Protein levels are normalized to the GAPDH loading control. Densitometry analysis is performed using the ImageJ software.

    Ceruletide purchased from MedChemExpress. Usage Cited in: Can J Physiol Pharmacol. 2019 Jan;97(1):15-22.  [Abstract]

    Expressions of Wnt/β-Catenin pathway-related genes in pancreatic and small intestinal tissues in each group (control, Caerulein, SKL2001+Caerulein, SKL2001+control). Relative mRNA expressions of Wnt1 (A, F), Wnt2 (B, G), β-catenin (C, H), c-Myc (D, I) and Axin-2 (E, J) in pancreatic and small intestinal tissues in each group detected by qRT-PCR.

    Ceruletide purchased from MedChemExpress. Usage Cited in: Am J Transl Res. 2018 Feb 15;10(2):402-410.  [Abstract]

    Cerulein exposure increases the protein expression of MD2, while ALR reduces the Cerulein-induced upregulation in MD2 protein expression.
    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Ceruletide is a decapeptide and a potent cholecystokinin receptor agonist. Ceruletide is a safe and effective cholecystokinetic agent with a direct spasmogenic effect on the gallbladder muscle and bile ducts[1].

    IC50 & Target

    Cholecystokinin receptor[4]

    In Vitro

    Ceruletide is similar chemically and biologically to the human gastrointestinal hormones cholecystokinin-pancreozymin (CCK) and gastrin II. Ceruletide stimulates gallbladder contraction, pancreatic exocrine secretion, gastric secretion, and motility in the distal duodenum, jejunum, ileum and colon, while delaying gastric emptying and inhibiting motility in the proximal duodenum[1]. Ceruletide in supramaximal but not in physiological doses activates NF-kappaB/Rel in vitro. This activation may induce a self-defending genetic program before the onset of cellular injury, which may prevent higher degrees of damage of pancreatic acinar cells after secretagogue hyperstimulation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Ceruletide can be used in animal modeling to construct animal models of pancreatitis.

    Ceruletide (0.4-0.5 mcg/kg, i.v.; 3-4 mcg/kg, s.c.) results in emesis and evacuation of the bowel in the intact conscious dog, and recovery is complete 15-30 min after i. v. administration and 2-4 hr after s.c. administration. Ceruletide (5-15 ng/kg, i.v.) shows a marked spasmogenic effect on the pylorus of rats. Ceruletide also reduces blood pressure in anesthetized dogs[1]. Ceruletide serum bile acid (SBA) stimulation circumvents exogenous and endogenous influences associated with postprandial (PP) SBA stimulation. Ceruletide SBA stimulation may perform as well as PP SBA stimulation in dogs with portosystemic shunt (PSS) and be more sensitive for the detection of hepatic dysfunction in dogs with upper respiratory disease (URD)[3].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Molecular Weight

    1352.41

    Formula

    C58H73N13O21S2

    CAS No.
    Appearance

    Solid

    Color

    White to off-white

    Sequence

    {pGlu}-Gln-Asp-Tyr(SO3H)-Thr-Gly-Trp-Met-Asp-Phe-NH2

    Sequence Shortening

    {pGlu}-QD-Y(SO3H)-TGWMDF-NH2

    Structure Classification
    Initial Source

    the skin of a tropical frog

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage

    Sealed storage, away from moisture

    Powder -80°C 2 years
    -20°C 1 year

    *In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (73.94 mM; Need ultrasonic)

    DMF : 16.67 mg/mL (12.33 mM; Need ultrasonic)

    H2O : 2.5 mg/mL (1.85 mM; Need ultrasonic)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 0.7394 mL 3.6971 mL 7.3942 mL
    5 mM 0.1479 mL 0.7394 mL 1.4788 mL
    10 mM 0.0739 mL 0.3697 mL 0.7394 mL
    *Please refer to the solubility information to select the appropriate solvent.
    In Vivo:
    • 1.

      Add each solvent one by one:  PBS

      Solubility: 30.3 mg/mL (22.40 mM); Clear solution; Need ultrasonic

    • 2.

      Add each solvent one by one:  Saline

      Solubility: 2 mg/mL (1.48 mM); Clear solution; Need ultrasonic and adjust pH to 12 with 1M NaOH

    • 3.

      Add each solvent one by one:  10% DMF    40% PEG300    5% Tween-80    45% Saline

      Solubility: ≥ 1.67 mg/mL (1.23 mM); Clear solution

    • 4.

      Add each solvent one by one:  10% DMF    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.67 mg/mL (1.23 mM); Clear solution

    • 5.

      Add each solvent one by one:  10% DMF    90% Corn Oil

      Solubility: 1.67 mg/mL (1.23 mM); Suspended solution; Need ultrasonic

    *All of the co-solvents are available by MedChemExpress (MCE).
    Purity & Documentation

    Purity: 99.96%

    References
    Animal Administration
    [3]

    Dogs[3]
    All dogs undergo serum bile acid (SBA) stimulation with food (<5 kg/body weight [BW] 2 teaspoons, >5 kg BW 2 tablespoons) or 0.3 μg/kg BW Ceruletide IM, respectively, on consecutive days. A diet of moderate protein content and with an increased concentration of fiber is chosen to minimize metabolic complications such as hepatic encephalopathy. Before each test, the dogs are fasted for 12 hours. Blood samples are drawn at baseline, 60 and 120 minutes after feeding, and 20, 30, and 40 minutes postinjection, respectively. The blood samples are collected in plain tubes and left to clot; they are then centrifuged at 6,500 ×g for 1 minute, and the serum is used to measure SBA by a colorimetric test with endpoint determination[3].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
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    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
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    Cat. No.:
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