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  4. Lipopolysaccharides, from E. coli O55:B5

Lipopolysaccharides, from E. coli O55:B5  (Synonyms: LPS)

Cat. No.: HY-D1056
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Lipopolysaccharides, from E. coli O55:B5 is an endotoxin extracted from E. coli O55:B5, consisting of an antigen-specific chain, A core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activates TLR-4 of immune cells. Lipopolysaccharides, from E. coli O55:B5 can induce the change of body temperature in rats with dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 caused a heterogeneous and dose-independent increase in body temperature in rats.

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Lipopolysaccharides, from E. coli O55:B5 Chemical Structure

Lipopolysaccharides, from E. coli O55:B5 Chemical Structure

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Top Publications Citing Use of Products

213 Publications Citing Use of MCE Lipopolysaccharides, from E. coli O55:B5

WB
Proliferation Assay
IF
RT-PCR

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650.  [Abstract]

    Western blot analysis of the phenotypic markers in LPS (1 µg/mL; 24 h)-stimulated macrophages.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2022 May 23;e2105650.  [Abstract]

    In LPS (1 µg/mL; 24 h)-stimulated macrophages, Representative fluorescence images of macrophage phenotypes after incubation with different pretreated neutrophils; iNOS (red), CD206 (green), and nuclei (blue).

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Biomater Res. 2022 Apr 25;26(1):15.  [Abstract]

    HUVECs ae induced with LPS 100 ng/mL for 24 h, then treated with PLCL-N, MPSS-loaded with 0.2 mg, 0.4 mg, 0.6 mg, 0.8 mg and 1 mg for another 24 h.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2022 Jan 21;13(1):73.  [Abstract]

    Effect of NKAP on the U87MG cell sensitivity to Erastin (10 μM), iFSP1 (100 μM), SAS (500 μM), Rotenone (2.5 μM), 17-DMAG (300 nM), Staurosporine (1.5 μM), TMZ (200 μM), β-lapachone (2 μM), H2O2 (1‰), LPS (200 μg/mL), and Rapamycin (300 nM). All drug treatments are for 24 h.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Sens Actuators B Chem. 15 December 2022, 132707.

    LPS is used as an external stimulus to induce •OH generation in the A549 and HeLa cell lines. Co-localization fluorescence imaging in A549 and HeLa cells using MTG and PY. Cells were incubated with LPS (10.0 μg/mL) for 24 h and then stained with MTG (500 nM) and PY (5.0 μM) for 30 min.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Elife. 2022 May 4;11:e76707.  [Abstract]

    qPCR analysis for the expression of indicated genes in D40 fVBOrs treated with lipopolysaccharide (LPS) (500 ng/mL; for 72 hr) without or with PLX5622 2 μM using DMSO as vehicle control.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Front Immunol. 2022 Jun 10;13:859806.  [Abstract]

    BMDMs are planted on XF24-well seahorse plates at the density of 5 × 104 cells per well and stimulated with 100 ng/mL LPS and 20 ng/mL hIFN-γ for 48 hours.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: Adv Funct Mater. 10 March 2022.

    The effect of CaNP on BMDM recruitment of T cells. Left panel: BMDM-M2 treated with CaNP (35 µg/mL) and LPS (50 ng/mL)/IFN-γ (20 ng/mL) for 48 h are in the lower compartment. CD8+ T cells are in the upper compartment. Right panel: representative images of BMDM recruitment of T cells. CD8+ T cells were labeled with FITC.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: J Med Virol. 2020 Aug 10.  [Abstract]

    The results show Apigenin significantly decreased 3pRNA‐ but not poly I:C‐ and LPS (0.5 μg/mL)‐induced A549 cell death.

    Lipopolysaccharides, from E. coli O55:B5 purchased from MedChemExpress. Usage Cited in: J Control Release. 2020 Jan 28;320:304-313.  [Abstract]

    Cells were differentiated to macrophages with PMA and then treated with 1 μg/mL LPS for 3 h.

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    Description

    Lipopolysaccharides, from E. coli O55:B5 is an endotoxin extracted from E. coli O55:B5, consisting of an antigen-specific chain, A core oligosaccharide, and lipid A. Lipopolysaccharides, from E. coli O55:B5 activates TLR-4 of immune cells. Lipopolysaccharides, from E. coli O55:B5 can induce the change of body temperature in rats with dose and serotype specificity. Lipopolysaccharides, from E. coli O55:B5 caused a heterogeneous and dose-independent increase in body temperature in rats[1][2][3][4][5].

    IC50 & Target

    TLR4

     

    In Vitro

    Lipopolysaccharides (10–80 μg/mL) selectively decreases THir (tyrosine hydroxylase immunoreactive) cells and increases culture media levels of interleukin1β (IL-1β) and tumor necrosis factor-α (TNF-α) as well as nitrite (an index of nitric oxide (NO) production)[5].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    In Vivo

    Induction of Cardiac Dysfunction[8]

    Background
    Cardiac dysfunction (cardiac inflammation) is a common complication of lipopolysaccharide (LPS)-induced sepsis, which is caused by severe inflammatory response. LPS can also cause deterioration of cardiac contractile function and increase cardiac lipid peroxidation, leading to cardiac dysfunction.
    Specific Mmodeling Methods
    Rat[8]: male • Wistar male rats • 6-week-old
    Administration: 10 mg/kg • ip for single dose • collected cardiac tissues and serums 24 hr after LPS-injection.
    Note
    /
    Modeling Record
    Phenotypic changes: Cardiac function left ventricular end-systolic volume (LVESV, %) and left ventricular end-diastolic volume (LVEDV, %) were significantly increased.
    Molecular level: The levels of CK-MB, LDH and AST in serum are ↑, and the TLR4/NF-κB pathway is inhibited.
    Correlated Product(s): Ferrostatin-1 (HY-100579)[7]

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Model: Female and male CD1 mice[3]
    Dosage: 1.5mg/kg
    Administration: Intraperitoneal injection, once
    Result: Induced sickness behavior in all mice, but adult mice displayed more sickness than pubertal mice and adult males remained sick for a longer period of time than adult females.
    Caused a decrease in body temperature for all mice, but this decrease was greatest in adult males.
    Increased pro- and anti-inflammatory cytokines at various levels in pubertal and adult male and female mice, resulted in age and sex differences in cytokine concentrations following immune challenge.
    Only adult males and females treated with LPS displayed significantly more IL-6 than their saline controls, and pubertal males and females and adult females displayed significantly more IL-10 than their saline controls.
    All the mice displayed significantly more IL-12 and TNF-α than their saline controls.
    Clinical Trial
    Appearance

    Solid

    Color

    White to off-white

    SMILES

    [Lipopolysaccharides, from E. coli O55:B5]

    Structure Classification
    Initial Source

    surface of Gram-negative bacteria

    Shipping

    Room temperature in continental US; may vary elsewhere.

    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 6 months
    -20°C 1 month
    Solvent & Solubility
    In Vitro: 

    H2O : 5 mg/mL (Need ultrasonic)

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    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: ≥ 1.43 mg/mL; Clear solution

      This protocol yields a clear solution of ≥ 1.43 mg/mL (saturation unknown).

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (14.3 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

    For the following dissolution methods, please prepare the working solution directly. It is recommended to prepare fresh solutions and use them promptly within a short period of time.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  PBS

      Solubility: 8.33 mg/mL; Clear solution; Need ultrasonic and warming and heat to 60°C

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    Purity & Documentation

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      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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    Product Name:
    Lipopolysaccharides, from E. coli O55:B5
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