Copy Link Print Information

IRG1 Antibody (YA4642)

(Synonyms: ACOD1 antibody; Aconitate decarboxylase 1 antibody; Aconitate decarboxylase antibody; CAD antibody; Cis aconitate decarboxylase antibody; Immune responsive gene 1 protein homolog antibody; Immune-responsive gene 1 protein homolog antibody; Immunoresponsive 1 homolog (mouse) antibody; Irg1 antibody; IRG1_HUMAN antibody; OC116 antibody ; OPTB1 antibody; TCTIRC7 antibody)
Customer Review

Based on 1 Customer Validation

IRG1 Antibody (YA4642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRG1.

For research use only. We do not sell to patients.
  • Host:

    Rabbit

  • Isotype:

    IgG

  • Application:

    WB, ICC/IF

  • Reactivity :

    Mouse

  • Formulation:

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Conjugation:
    Non-conjugated
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • IRG1 Antibody (YA4642) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download
Related Products:
Top Publications Citing

Use of MCE Products

Applications

Application
WB Info
WB: Western Blot
ICC/IF Info
ICC/IF: Immunocytochemistry/
Immunofluorescence
Dilution Ratio 1:1000 1:100

Product Details

Description

IRG1 Antibody (YA4642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRG1.

  • Host Rabbit
  • Clonality Monoclonal,Recombinant
  • Species Reactivity
    Mouse
  • Observed Molecular Weight
    Observed band size: 54 kDa Info
    Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.
  • Calculated Molecular Weight Predicted band size: 54 kDa
Species Reactivity Database

SwissProt: P54987 Mouse

Immunogen

Recombinant protein within mouse IRG1 aa 1-488.

Purification

affinity purified

Conjugation

Non-conjugated

Modification

Unmodified

Isotype

IgG

Product Properties

  • Appearance

    Solution

  • Formulation

    Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

  • Concentration

    Batch-dependent, Please check the COA for the concentration of each lot. Check Lot Concentration

  • Storage & Stability

    Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

  • Shipping

    Shipping with blue ice.

Verification Images

  • Experimental Validation Results for IRG1 Antibody (YA4642)
    RAW264.7 cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with LPS (HY-D1056) at concentrations ranging from 0 to 1 μg/mL for 4 h. IRG1 protein expression was subsequently analyzed by Western blot. The results demonstrated that LPS induced IRG1 expression in a dose-dependent manner, confirming the responsiveness of the model. Primary antibodies used: IRG1 Antibody (HY-P84946) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for IRG1 Antibody (YA4642)
    Western blot analysis of extracts from Raw264.7 (lane 2 (25 μg), LPS treated (HY-D1056, 0.1 μg/mL, 4 h) Raw264.7 cells, and LPS treated (1 μg/mL, 4 h) Raw264.7 cells as indicated using IRG1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

Background

  • Function

    IRG1 is a Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response. Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages. Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB. Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes. Plays a role in the embryo implantation[1][2][3][4][5][6].

  • Subcellular Localization

    Mitochondrion

  • Expression


    Tissue_specificity:Expression in LPS-tolerant macrophages (protein level) (PubMed:23609450, PubMed:35662396) . Expression in pregnant uterine epithelial cells (PubMed:14500577) . Expression in microglia and macrophages (PubMed:23609450, PubMed:23610393, PubMed:35662396) .

    Induction:Up-regulated after lipopolysaccharide (LPS) stimulation (PubMed:23609450, PubMed:23610393) . Up-regulated in LPS-tolerized macrophage by LPS (PubMed:23609450, PubMed:23610393) . Up-regulated synergistically by pro-inflammatory cytokines TNF and IFNG (PubMed:19014335) . Up-regulated by pro-inflammatory cytokines IL1B and IFNB1 (PubMed:19014335) . Up-regulated by progesterone and at the time of the embryo implantation (PubMed:14500577) . Expression is activated by IRF1 in neurons in response to flavivirus infection in neurons (PubMed:30635240)

  • Subunit

    Homodimer

  • SwissProt ID

    P54987

  • Gene ID
    16365 [NCBI]
  • Synonyms

    ACOD1 antibody; Aconitate decarboxylase 1 antibody; Aconitate decarboxylase antibody; CAD antibody; Cis aconitate decarboxylase antibody; Immune responsive gene 1 protein homolog antibody; Immune-responsive gene 1 protein homolog antibody; Immunoresponsive 1 homolog (mouse) antibody; Irg1 antibody; IRG1_HUMAN antibody; OC116 antibody ; OPTB1 antibody; TCTIRC7 antibody

IRG1 Antibody (YA4642) Related Classifications

MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     RAW264.7 cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with LPS (HY-D1056) at concentrations ranging from 0 to 1 μg/mL for 4 h. IRG1 protein expression was subsequently analyzed by Western blot. The results demonstrated that LPS induced IRG1 expression in a dose-dependent manner, confirming the responsiveness of the model. Primary antibodies used: IRG1 Antibody (HY-P84946) and GAPDH Antibody (HY-P80954A).

  • 1/16

    MedChemExpress Validation
     Western blot analysis of extracts from Raw264.7 (lane 2 (25 μg), LPS treated (HY-D1056, 0.1 μg/mL, 4 h) Raw264.7 cells, and LPS treated (1 μg/mL, 4 h) Raw264.7 cells as indicated using IRG1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IRG1 Antibody (YA4642) Product Image
    1/16
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • IRG1 Antibody (YA4642) Product Image
  • Experimental Validation Results for IRG1 Antibody (YA4642)
    1/1
    MedChemExpress Validation
     RAW264.7 cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with LPS (HY-D1056) at concentrations ranging from 0 to 1 μg/mL for 4 h. IRG1 protein expression was subsequently analyzed by Western blot. The results demonstrated that LPS induced IRG1 expression in a dose-dependent manner, confirming the responsiveness of the model. Primary antibodies used: IRG1 Antibody (HY-P84946) and GAPDH Antibody (HY-P80954A).
  • Experimental Validation Results for IRG1 Antibody (YA4642)
    1/1
    MedChemExpress Validation
     Western blot analysis of extracts from Raw264.7 (lane 2 (25 μg), LPS treated (HY-D1056, 0.1 μg/mL, 4 h) Raw264.7 cells, and LPS treated (1 μg/mL, 4 h) Raw264.7 cells as indicated using IRG1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • Experimental Validation Results for IRG1 Antibody (YA4642)
  • IRG1 Antibody (YA4642) Product Image
    MedChemExpress Validation

    RAW264.7 cells were cultured in 6 cm dishes to approximately 70% confluence and then treated with LPS (HY-D1056) at concentrations ranging from 0 to 1 μg/mL for 4 h. IRG1 protein expression was subsequently analyzed by Western blot. The results demonstrated that LPS induced IRG1 expression in a dose-dependent manner, confirming the responsiveness of the model. Primary antibodies used: IRG1 Antibody (HY-P84946) and GAPDH Antibody (HY-P80954A).

  • IRG1 Antibody (YA4642) Product Image
    MedChemExpress Validation

    Western blot analysis of extracts from Raw264.7 (lane 2 (25 μg), LPS treated (HY-D1056, 0.1 μg/mL, 4 h) Raw264.7 cells, and LPS treated (1 μg/mL, 4 h) Raw264.7 cells as indicated using IRG1 antibody. Proteins were transferred to a PVDF membrane and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. The primary antibody (1/1000) and Loading control antibody (GAPDH, HY-P80954A, 1/5000) was used in 5% Bovine Serum Albumin in TBST at 4°C overnight. After washing, the membrane of primary antibody was incubated with HRP-conjugated goat anti-rabbit/mouse IgG secondary antibody (HY-P8001/HY-P8004) (1:5000) diluted in 5% nonfat milk in TBST for 1 h at room temperature. Protein bands were visualized using an Ultra High Sensitivity ECL detection kit (HY-K1005).

  • IRG1 Antibody (YA4642) Product Image WB
  • IRG1 Antibody (YA4642) Product Image WB