IRG1 Antibody (YA4642)

Art. -Nr.: HY-P84946: Data Sheet COA
SDS
User Guide for Antibodies
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Technical Support

IRG1 Antibody (YA4642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRG1.

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Größe	Verfügbarkeit	Menge
20 μL	Auf Lager	Estimated Time of Arrival: December 31
50 μL	Auf Lager	Estimated Time of Arrival: December 31
100 μL	Auf Lager	Estimated Time of Arrival: December 31
250 μL	Angebot einholen

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WB: Western Blot;
IHC-P: Immunohistochemistry-Paraffin;
IHC-F: Immunohistochemistry-Frozen;
ICC/IF: Immunocytochemistry/Immunofluorescence;
IF-Tissue: Immunofluorescence-Tissue;
mIHC: Multiplex Immunohistochemical;
IP: Immunoprecipitation;
ChIP: Chromatin Immunoprecipitation;
FC: Flow Cytometry;
ELISA: Enzyme Linked Immunosorbent Assay

Product Detail
Background
Beschreibung

Beschreibung

IRG1 Antibody (YA4642) is a Rabbit-derived and non-conjugated IgG monoclonal antibody, targeting to IRG1.

Host

Rabbit

Clonality

Monoclonal, Recombinant

Molekulargewicht

Predicted band size: 54 kDa;

Observed band size: 54 kDa

Note: Due to possible protein modifications or aggregation, the molecular weight should be confirmed by actual measurement, and the predicted value is for reference only.

Species Reactivity

Mouse

SwissProt ID

P54987

Gene ID

16365 [NCBI]

Immunogen

Recombinant protein within mouse IRG1 aa 1-488.

Application &
Dilution Ratio

Application	Dilution Ratio
WB WB: Western Blot	1:1000
ICC/IF ICC/IF: Immunocytochemistry/Immunofluorescence	1:100

Reinheit	affinity purified	Conjugation	Non-conjugated
Modification	Unmodified	Isotype	IgG

Appearance

Liquid

Formulation

Supplied in PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Storage & Stability

Stored at -20°C for 1 year. Avoid repeated freeze / thaw cycles.

Versand

Shipping with blue ice.

Background

Function：Cis-aconitate decarboxylase that catalyzes production of itaconate and is involved in the inhibition of the inflammatory response (PubMed:23609450, PubMed:23610393, PubMed:30635240, PubMed:31548418). Acts as a negative regulator of the Toll-like receptors (TLRs)-mediated inflammatory innate response by stimulating the tumor necrosis factor alpha-induced protein TNFAIP3 expression via reactive oxygen species (ROS) in LPS-tolerized macrophages (PubMed:23609450). Involved in antimicrobial response of innate immune cells; ACOD1-mediated itaconic acid production contributes to the antimicrobial activity of macrophages by generating itaconate, leading to alkylation of proteins, such as TFEB (PubMed:23610393, PubMed:35662396). Involved in antiviral response following infection by flavivirus in neurons: ACOD1-mediated itaconate production inhibits the activity of succinate dehydrogenase, generating a metabolic state in neurons that suppresses replication of viral genomes (PubMed:30635240). Plays a role in the embryo implantation (PubMed:14500577)

Subcellular Localization：Mitochondrion

Expression：
Tissue_specificity:Expression in LPS-tolerant macrophages (protein level) (PubMed:23609450, PubMed:35662396) . Expression in pregnant uterine epithelial cells (PubMed:14500577) . Expression in microglia and macrophages (PubMed:23609450, PubMed:23610393, PubMed:35662396) .

Induction:Up-regulated after lipopolysaccharide (LPS) stimulation (PubMed:23609450, PubMed:23610393) . Up-regulated in LPS-tolerized macrophage by LPS (PubMed:23609450, PubMed:23610393) . Up-regulated synergistically by pro-inflammatory cytokines TNF and IFNG (PubMed:19014335) . Up-regulated by pro-inflammatory cytokines IL1B and IFNB1 (PubMed:19014335) . Up-regulated by progesterone and at the time of the embryo implantation (PubMed:14500577) . Expression is activated by IRF1 in neurons in response to flavivirus infection in neurons (PubMed:30635240)

Subunit：Homodimer

Database

SwissProt: P54987 Mouse

Synonyms

ACOD1 antibody; Aconitate decarboxylase 1 antibody; Aconitate decarboxylase antibody; CAD antibody; Cis aconitate decarboxylase antibody; Immune responsive gene 1 protein homolog antibody; Immune-responsive gene 1 protein homolog antibody; Immunoresponsive 1 homolog (mouse) antibody; Irg1 antibody; IRG1_HUMAN antibody; OC116 antibody ; OPTB1 antibody; TCTIRC7 antibody

Dokumentation

Select Batch:

Data Sheet (234 KB) SDS (252 KB)

COA (206 KB)

User Guide for Antibodies (1077 KB)