Two previously undescribed phthalides from Talaromyces amestolkiae, a symbiotic fungus of Syngnathus acus

  • J Asian Nat Prod Res. 2022 May 18;1-9. doi: 10.1080/10286020.2022.2075738.
Li-Jun Huang  1 ,  Xin-Ai Li  2 ,  Meng-Ying Jin  2 ,  Wen-Xiu Guo  2 ,  Li-Rong Lei  2 ,  Ran Liu  2 ,  Ming-Zhi Zhang  3 ,  Da-Le Guo  2 ,  Dong Wang  1  2 ,  Yan Zhou  4 ,  Yun Deng  2 ,  Jian-Guang Zhang  5
Affiliations
  • 1. School of Basic Medical Sciences, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
  • 2. State Key Laboratory of Southwestern Chinese Medicine Resources, School of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
  • 3. Jiangsu Key Laboratory of Pesticide Science, College of Sciences, Nanjing Agricultural University, Nanjing 210095, China.
  • 4. Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, People's Republic of China.
  • 5. Qinzhou Provincial Health School, Qinzhou 535000, China.
PMID: 35582859 DOI: 10.1080/10286020.2022.2075738
Abstract

Amestolkins A (1) and B (2), two previously undescribed phthalides sharing the same planar structure of (1, 5-dihydroxyhexyl)-7-hydroxyisobenzofuran-1(3H)-one were isolated from Talaromyces amestolkiae. Their absolute configurations were elucidated by comprehensive analyses of spectroscopic evidences in high-resolution electrospray mass spectra (HRESIMS) and nuclear magnetic resonance (NMR) combined with electronic circular dichroism (ECD) and NMR calculations. 1 and 2 showed anti-neuroinflammatory activity by inhibiting the gene expressions of proinflammatory factors including C-C motif chemokine ligand 2 (CCL-2), tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), as well as attenuating the excretion of inducible nitric oxide synthase (iNOS) in BV-2 microglial cells at the concentration of 30 μM.

Keywords
Trichocomaceae; Talaromyces amestolkiae; absolute configuration; anti-neuroinflammation; phthalide.
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