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  4. Coppersensor 1

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases.

For research use only. We do not sell to patients.

CAS No. : 874748-20-6

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
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Based on 29 publication(s) in Google Scholar

Other Forms of Coppersensor 1:

Coppersensor 1 Related Antibodies

Top Publications Citing Use of Products

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 13:e02501.  [Abstract]

    Analysis of monovalent copper ion levels in TRIM21‐knockdown KYSE30 cells following 0.5 mm CuSO4 treatment (CS1, 5 μM, 10 min).

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Jul 13:e02501.  [Abstract]

    Analysis of monovalent copper ion levels in TRIM21‐knockdown KYSE450 cells following 0.5 mm CuSO4 treatment (CS1, 5 μM, 10 min).

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Stem Cell Res Ther. 2025 Sep 29;16(1):526.  [Abstract]

    Copper sensor probe (Red) (5 μM; 30 min) was used.

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Int J Mol Med. 2025 Oct;56(4):154.  [Abstract]

    Copper sensor 1 (5 μM; 30 min). Fluorescence staining indicated that intracellular copper ion levels were elevated in U87 and U251 cells following SDF2 knockdown compared with those in control cells (n = 3, scale bar, 50 µm).

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Aug 13;15(1):6947.  [Abstract]

    RNA-qPCR fold-changes of select transcripts 4 h post-supplementation of 500 nM CuGTSM or 5 µM CS1 relative to DMSO treated A549 cells.

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Mater Today Bio. 2024 May 11:26:101087.

    Representative fluorescent images of intracellular Cu⁺ distribution. Upon exposure to HL stimulation for 12 h, NIH-3T3 cells were treated with CuONPs or CuO@PS, both containing CuONPs at concentrations of 5 μg/mL, for 12 h, and then the cells were washed and detected with Cu⁺ probe Coppersensor-1 (CS-1, red) (5 μM; 20 min) at different time points. Lysosomes were stained with lysotracker (green). Scale bar, 10 μm.

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: Int J Mol Sci. 2024 Oct 30;25(21):11657.  [Abstract]

    Copper content was measured using Copper sensor-1 (5 μM; 30 min) and detected by fluorescence microscopy.

    Coppersensor 1 purchased from MedChemExpress. Usage Cited in: BMC Pharmacol Toxicol. 2024 Aug 21;25(1):54.  [Abstract]

    Levels of copper in cells were labeled by copper sensor-1 (5 μM; 15 min).

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu+ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu+) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases[1].

    In Vitro

    Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1][2].
    Cell Labeling::
    1. Prepare a reagent stock solution for imaging: Prepare a 1 mM CS1 stock solution in DMSO.
    2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
    3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer.
    4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.
    5. Incubate for 5-20 min in the dark at 25 or 37°C.
    6. Imageing in standard confocal microscope with 543 nm excitation.
    (CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    Coppersensor 1 Related Antibodies
    Clinical Trial
    Molecular Weight

    629.81

    Formula

    C30H50BF2N3S4
    CAS No.
    Appearance

    Solid

    Color

    Brown to red

    Emission (Em)

    576
    Excitation (Ex)

    543
    SMILES

    [F-][B+3]1([N]2=C(C)C(CC)=C(C)C2=C(CN(CCSCCSCC)CCSCCSCC)C3=C(C)C(CC)=C(C)[N-]31)[F-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 25 mg/mL (39.69 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 1.5878 mL 7.9389 mL 15.8778 mL
    5 mM 0.3176 mL 1.5878 mL 3.1756 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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    =
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    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

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    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation

    Purity: ≥98.0%

    SDS (252 KB)

    COA (252 KB) HNMR (268 KB) MS (69 KB)

    Handling Instructions (2659 KB)
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 1.5878 mL 7.9389 mL 15.8778 mL 39.6945 mL
    5 mM 0.3176 mL 1.5878 mL 3.1756 mL 7.9389 mL
    10 mM 0.1588 mL 0.7939 mL 1.5878 mL 3.9695 mL
    15 mM 0.1059 mL 0.5293 mL 1.0585 mL 2.6463 mL
    20 mM 0.0794 mL 0.3969 mL 0.7939 mL 1.9847 mL
    25 mM 0.0635 mL 0.3176 mL 0.6351 mL 1.5878 mL
    30 mM 0.0529 mL 0.2646 mL 0.5293 mL 1.3232 mL
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    Coppersensor 1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    Coppersensor 1874748-20-6Coppersensor1Coppersensor-1Fluorescent Dyemembrane-permeableboron dipyrromethene (BODIPY)fluorescent dyebiological imagingcancercardiovascular disordersneurogenerative diseasesInhibitorinhibitorinhibit

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