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Fluo-3AM  (Synonyms: Fluo-3-pentaacetoxymethyl ester)

Cat. No.: HY-D0716 Purity: 96.92%
COA Handling Instructions

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence.

For research use only. We do not sell to patients.

Fluo-3AM Chemical Structure

Fluo-3AM Chemical Structure

CAS No. : 121714-22-5

Size Price Stock Quantity
500 μg USD 350 In-stock
1 mg USD 520 In-stock
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10 mg   Get quote  

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Customer Review

Based on 5 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

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Description

Fluo-3 AM is a fluorecent Ca2+ chelator, with high affinity for calcium. Fluo-3 AM can specifically identify intracellular calcium ions, with high sensitivity, low cytotoxicity, increased AM acetylmethyl ester can enter the cell well, after being sheared by the intracellular esterase stay in the cell to bind to calcium ions, produce strong fluorescence[1].

In Vitro

1. Preparation of Fluo-3 AM working solution
1.1 Preparation of the stock solution
Dissolve 1 mg Fluo-3 AM in 135 μL DMSO to obtain 10 mM of stock solution.
Note: It is recommended to store the stock solution at -20°C or -80°C away from light and avoid repetitive freeze-thaw cycles.
1.2 Preparation of Fluo-3 AM working solution
Dilute the stock solution in HBSS to obtain 1-10 μM of working solution.
Note: Please adjust the concentration of Fluo-3 AMworking solution according to the actual situation.
2. Cell staining
2.1 Suspension cells (6-well plate)
a. Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL
b.Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
c. Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
d. Wash twice with PBS, 5 minutes each time.
e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
2.2 Adherent cells
a. Culture adherent cells on sterile coverslips.
b. Remove the coverslip from the medium and aspirate excess medium.
c. Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
d. Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.
Storage
-20°C, 1 year
Protect from light

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1129.85

Formula

C51H50Cl2N2O23

CAS No.
Appearance

Solid

Color

Brown to reddish brown

Emission (Em)

526

Excitation (Ex)

488

SMILES

ClC(C=C1C(C2=CC(OCCOC(C=C(C)C=C3)=C3N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C2)=C(C=C4Cl)C5=CC4=O)=C(C=C1O5)OCOC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : 12.5 mg/mL (11.06 mM; Need ultrasonic)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.8851 mL 4.4254 mL 8.8507 mL
5 mM 0.1770 mL 0.8851 mL 1.7701 mL
10 mM 0.0885 mL 0.4425 mL 0.8851 mL
*Please refer to the solubility information to select the appropriate solvent.
Purity & Documentation

Purity: ≥99.0%

Dyeing Example
References
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Fluo-3AM Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

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Fluo-3AM
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