2. Others
  3. Fluorescent Dye
  4. RhoNox-1

RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus.

For research use only. We do not sell to patients.

CAS No. : 1447815-38-4

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
Solid
5 mg In-stock
10 mg In-stock
25 mg In-stock
50 mg In-stock
100 mg In-stock
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 39 publication(s) in Google Scholar

Other Forms of RhoNox-1:

RhoNox-1 Related Antibodies

Top Publications Citing Use of Products

39 Publications Citing Use of MCE RhoNox-1

Flow Cytometry
Cell Imaging/Staining

    RhoNox-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Nov 10:e10991.  [Abstract]

    RhoNox‐1 (10 µM; 30 min). The levels of Fe2+ in BMDMs were analyzed by immunostaining and flow cytometry analysis of FeRhoNox-1.

    RhoNox-1 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 May 21:e2505359.  [Abstract]

    Intracellular Fe2+ levels under 30 µM Erastin or 500 µM FAC (Ammonium iron(III) citrate) treatment for 4 h were detected with the RhoNox-1 (1μM; 1 h) fluorescent probe.

    RhoNox-1 purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Nov 5;23(1):703.  [Abstract]

    RhoNox‐1 (1 µM; 30 min). Representative images of HK-2 cells stained with RhoNox-1 probe.

    RhoNox-1 purchased from MedChemExpress. Usage Cited in: ACS Appl Mater Interfaces. 2025 Oct 22;17(42):57903-57918.  [Abstract]

    RhoNox-1 (10 μM; 1 h). Fluorescence images showing intracellular Fe²⁺ (red; FeRhoNox-1) and nuclear staining (blue; Hoechst) in B16 cells after incubation with PFE-PEG NPs or PFE-PEG NPs plus NIR irradiation.

    RhoNox-1 purchased from MedChemExpress. Usage Cited in: J Med Chem. 2025 Apr 24;68(8):8124-8142.  [Abstract]

    RhoNox-1 (5 μM; 20 min). Flow cytometry analysis for intracellular Fe2+ levels (RhoNox-1) in U937 cell treated with DMSO or A9 for 48 h.

    • Biological Activity

    • Purity & Documentation

    • References

    • Customer Review

    Description

    RhoNox-1 is a fluorescent probe for the specific detection of divalent iron ions, and when RhoNox-1 reacts with Fe2+. RhoNox-1 can generate an irreversible orange (red) fluorescent product (Ex/Em：540/575 nm) . FeRhoNox-1 can enter the cell well, suitable for the detection of Fe2+ in living cells, and tends to be localized in the Golgi apparatus[1].

    In Vitro

    Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
    1. Preparation of RhoNox-1 working solution
    1.1 Preparation of the stock solution
    Dissolve RhoNox-1 in DMSO to obtain 1 mM of stock solution.
    1.2 Preparation of RhoNox-1 working solution
    Dilute the stock solution in serum-free cell culture medium or PBS to obtain 1-10 μM of working solution.
    Note: Please adjust the concentration of RhoNox-1 working solution according to the actual situation.
    2. Cell staining (Suspension cells)
    2.1 Centrifuge at 1000 g at 4°C for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL.
    2.2 Add 1 mL of working solution, and then incubate at room temperature for 5-30 minutes.
    2.3 Centrifuge at 400 g at 4°C for 3-4 minutes and then discard the supernatant.
    2.4 Wash twice with PBS, 5 minutes each time.
    2.5 Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry.
    3. Cell staining (Adherent cells)
    3.1 Culture adherent cells on sterile coverslips.
    3.2 Remove the coverslip from the medium and aspirate excess medium.
    3.3 Add 100 μL of working solution, gently shake it to completely cover the cells, and then incubate at room temperature for 5-30 minutes.
    3.4 Wash twice with medium, 5 minutes each time. Observation by fluorescence microscopy.

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    RhoNox-1 Related Antibodies
    Molecular Weight

    458.55

    Formula

    C28H30N2O4
    CAS No.
    Appearance

    Solid

    Color

    Pink to red

    Emission (Em)

    575
    Excitation (Ex)

    540
    SMILES

    O=C(C1=C(C2=C3C=C/C(C=C3OC4=CC([N+](CC)(CC)[O-])=CC=C42)=[N+](CC)\CC)C=CC=C1)[O-]
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : 100 mg/mL (218.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 2.1808 mL 10.9039 mL 21.8079 mL
    5 mM 0.4362 mL 2.1808 mL 4.3616 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 2.1808 mL 10.9039 mL 21.8079 mL 54.5197 mL
    5 mM 0.4362 mL 2.1808 mL 4.3616 mL 10.9039 mL
    10 mM 0.2181 mL 1.0904 mL 2.1808 mL 5.4520 mL
    15 mM 0.1454 mL 0.7269 mL 1.4539 mL 3.6346 mL
    20 mM 0.1090 mL 0.5452 mL 1.0904 mL 2.7260 mL
    25 mM 0.0872 mL 0.4362 mL 0.8723 mL 2.1808 mL
    30 mM 0.0727 mL 0.3635 mL 0.7269 mL 1.8173 mL
    40 mM 0.0545 mL 0.2726 mL 0.5452 mL 1.3630 mL
    50 mM 0.0436 mL 0.2181 mL 0.4362 mL 1.0904 mL
    60 mM 0.0363 mL 0.1817 mL 0.3635 mL 0.9087 mL
    80 mM 0.0273 mL 0.1363 mL 0.2726 mL 0.6815 mL
    100 mM 0.0218 mL 0.1090 mL 0.2181 mL 0.5452 mL
    • No file chosen (Maximum size is: 1024 Kb)
    • If you have published this work, please enter the PubMed ID.
    • Your name will appear on the site.

    RhoNox-1 Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    RhoNox-11447815-38-4RhoNox1RhoNox 1Fluorescent Dyefluorescent probesLIPMMNK1HepG2 cellsiron metabolismInhibitorinhibitorinhibit

    Your Recently Viewed Products:

    Inquiry Online

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Requested Quantity *

    Applicant Name *

    		 

    Salutation

    Email Address *

    		 

    Phone Number *

    Department

    		 

    Organization Name *

    City

    State

    Country or Region *

    		      

    Remarks

    Bulk Inquiry

    Inquiry Information

    Product Name:
    RhoNox-1
    Cat. No.:
    HY-D1533
    Quantity:
    MCE Japan Authorized Agent:

    Customer Review

    Thank You for Your Feedback!

    Request for HNMR Report

    Please fill out this form to request the QC report. We will send it to your Email address shortly.

    Your information is safe with us. * Required Fields.

    Product Name

    		  

    Lot #

    Applicant Name *

    		 

    Email Address *

    Phone Number

    		 

    Department *

    Organization Name *

    		 

    Country or Region *

    Remarks

    SAFETY DATA SHEET (SDS)

    English - EN (252 KB) Français - FR (252 KB) Deutsch - DE (252 KB) Norwegian - NO (252 KB) Español - ES (252 KB) Swedish - SV (252 KB) Italian - IT (252 KB) Korean - KR (252 KB) Portuguese - PT (252 KB)

    Request for HNMR Report

    We have received your request and will respond to you as soon as possible.