Zinquin ethyl ester

Name: Zinquin ethyl ester
Brand: MedChemExpress
SKU: HY-124171
Rating: 4.5 (1 reviews)

Cat. No.: HY-124171 Purity: 99.0%: Data Sheet
COA

SDS
Handling Instructions
FAQs
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Zinquin ethyl ester is a cell-permeable and lipophilic fluorescent derivative of Zinquin (HY-D0982). Zinquin ethyl ester reacts with protein-bound Zn²⁺ in cells and forms fluorescent ternary adducts. Zinquin ethyl ester undergoes hydrolysis by intracellular esterases impeding its efflux across the plasma membrane (Ex/Em = 370/470 nm).

For research use only. We do not sell to patients.

Zinquin ethyl ester Chemical Structure

CAS No. : 181530-09-6

Size	Stock	Quantity
1 mg	In-stock	Estimated Time of Arrival: December 31
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	Get quote
50 mg	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

1 Publications Citing Use of MCE Zinquin ethyl ester

•Colloids Surf B Biointerfaces. 2025 Feb 8:250:114549. [Abstract]

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Biological Activity
Purity & Documentation
References
Customer Review

Description

In Vitro

Guidelines (The following is our recommended protocol; this protocol serves as a guideline only and should be modified to suit your specific needs.)
1. Preparation of Stock and Working Solutions
a. Prepare a 10 mM Zinquin ethyl ester stock solution in anhydrous DMSO.
b. Dilute the stock solution using pre-warmed, serum-free cell culture medium or PBS to prepare a 25 μM Zinquin ethyl ester working solution.
Note: Please adjust the concentration of the Rhobo6 working solution according to your specific experimental requirements, and prepare it immediately before use.
2. Cell Staining
a. Culture cells on sterile coverslips.
b. Remove the coverslips from the culture medium and aspirate any excess medium.
c. Add the working solution, gently agitating to ensure it completely covers the cells, and incubate at room temperature for 30 minutes.
d. Aspirate the working solution, then wash 2–3 times with culture medium (5 minutes per wash). Observe using a fluorescence microscope or flow cytometer.
Note: If analysis via flow cytometry is required, the cells must be detached using trypsin digestion and resuspended prior to staining.
Zinquin ethyl ester (25 μM; 30 min) forms fluorescent ternary adducts with Zn-proteins in LLC-PK1, TE671, U87 mg, C6, CCRF-CEM, A549, and mouse primary brain cells, producing a characteristic emission maximum near 470 nm with cell-type specific fluorescence intensities^[1].
Zinquin ethyl ester (1-20 μM; 15 min) forms fluorescent ternary adducts with the isolated Zn-proteome from TE671 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

414.47

Formula

C₂₁H₂₂N₂O₅S

CAS No.

181530-09-6

Appearance

Solid

Color

White to off-white

Emission (Em)

490

Excitation (Ex)

368

SMILES

O=C(OCC)COC1=CC(NS(=O)(C2=CC=C(C)C=C2)=O)=C3N=C(C)C=CC3=C1

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : ≥ 50 mg/mL (120.64 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.4127 mL	12.0636 mL	24.1272 mL
5 mM	0.4825 mL	2.4127 mL	4.8254 mL
10 mM	0.2413 mL	1.2064 mL	2.4127 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.0%

Select Batch:

Data Sheet (276 KB) SDS (252 KB)

COA (250 KB)

Handling Instructions (2659 KB)

References

[1]. Nowakowski AB, et al. Reactions of the fluorescent sensor, Zinquin, with the zinc-proteome: adduct formation and ligand substitution. Inorg Chem. 2011;50(20):10124-10133. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.4127 mL	12.0636 mL	24.1272 mL	60.3180 mL
	5 mM	0.4825 mL	2.4127 mL	4.8254 mL	12.0636 mL
	10 mM	0.2413 mL	1.2064 mL	2.4127 mL	6.0318 mL
	15 mM	0.1608 mL	0.8042 mL	1.6085 mL	4.0212 mL
	20 mM	0.1206 mL	0.6032 mL	1.2064 mL	3.0159 mL
	25 mM	0.0965 mL	0.4825 mL	0.9651 mL	2.4127 mL
	30 mM	0.0804 mL	0.4021 mL	0.8042 mL	2.0106 mL
	40 mM	0.0603 mL	0.3016 mL	0.6032 mL	1.5079 mL
	50 mM	0.0483 mL	0.2413 mL	0.4825 mL	1.2064 mL
	60 mM	0.0402 mL	0.2011 mL	0.4021 mL	1.0053 mL
	80 mM	0.0302 mL	0.1508 mL	0.3016 mL	0.7540 mL
	100 mM	0.0241 mL	0.1206 mL	0.2413 mL	0.6032 mL