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Fura-2 AM (Synonyms: Fura-2 Acetoxymethyl ester)

Cat. No.: HY-101897 Purity: ≥99.0%
Handling Instructions

Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator.

For research use only. We do not sell to patients.

Fura-2 AM Chemical Structure

Fura-2 AM Chemical Structure

CAS No. : 108964-32-5

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1 mg USD 400 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 3 publication(s) in Google Scholar

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  • Biological Activity

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Description

Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator.

In Vitro

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid.
1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months.
2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min.
3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish.
4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely.
5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish.
6. Mount the coverslip on the imaging chamber.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

1001.85

Formula

C₄₄H₄₇N₃O₂₄

CAS No.

108964-32-5

SMILES

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light

*The compound is unstable in solutions, freshly prepared is recommended.

References
Cell Assay

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References

Purity: ≥99.0%

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Keywords:

Fura-2 AMFura-2 Acetoxymethyl esterOthersInhibitorinhibitorinhibit

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Fura-2 AM
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