1. Dye Reagents

Fura-2 AM (Synonyms: Fura-2 Acetoxymethyl ester)

Cat. No.: HY-101897 Purity: >99.0%
Handling Instructions

Fura-2 AM is a ratiometric fluorescent Ca2+ indicator.

For research use only. We do not sell to patients.

Fura-2 AM Chemical Structure

Fura-2 AM Chemical Structure

CAS No. : 108964-32-5

Size Price Stock Quantity
1 mg USD 312 In-stock
Estimated Time of Arrival: December 31
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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References

Description

Fura-2 AM is a ratiometric fluorescent Ca2+ indicator.

In Vitro

The Ca2 increases above the baseline for the 750, 500 and 250 nM are 633.0±33.9 nM (n=111, P>0.05 compare to 1 μM Fura-2 AM), 552.6±22.7 nM (n=119, P>0.05 compare to 1 μM Fura-2 AM) and 616.0±24.9 nM (n=130, P>0.05 compare to 1 μM Fura-2 AM), respectively[1].

Solvent & Solubility
In Vitro: 

10 mM in DMSO

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 0.9982 mL 4.9908 mL 9.9815 mL
5 mM 0.1996 mL 0.9982 mL 1.9963 mL
10 mM 0.0998 mL 0.4991 mL 0.9982 mL
*Please refer to the solubility information to select the appropriate solvent.
References
Cell Assay
[2]

Cells are loaded with Fura-2 AM, which diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. Aliquot 2-mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1 µM Fura-2 AM solution. Vortex the solution vigorously for 1 minute. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Prepare a 35 mm dish containing 2-mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. Mount the coverslip on the imaging chamber[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
Molecular Weight

1001.85

Formula

C₄₄H₄₇N₃O₂₄

CAS No.

108964-32-5

SMILES

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O

Storage

-20°C, protect from light

Shipping

Room temperature in continental US; may vary elsewhere

Purity: >99.0%

  • Molarity Calculator

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The dilution calculator equation

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This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

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Product Name:
Fura-2 AM
Cat. No.:
HY-101897
Quantity:

Fura-2 AM

Cat. No.: HY-101897