Fura-2 pentapotassium
Based on 1 Customer Validation
Fura-2 pentapotassium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentapotassium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca2+ level.
For research use only. We do not sell to patients.
- Purity: 97.0%
- CAS No.: 113694-64-7
- Formula: C29H22K5N3O14
- Molecular Weight:831.99
-
Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Biological Activity
Fura-2 itself cannot pass through the cell membrane, and various methods (such as microinjection, ATP permeabilization, scrape loading, and pH shock) can be employed to insert Fura-2 into the cytoplasm of cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
540
510
Chemical Information
-
CAS No. 113694-64-7
-
Appearance Solid
-
Molecular Weight 831.99
-
Formula C29H22K5N3O14
-
Color Off-white to light yellow
-
SMILES
O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(O[K])=O)CC(O[K])=O)=C(N(CC(O[K])=O)CC(O[K])=O)C=C3O2)O1)O[K]
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
1M NaOH : 10 mg/mL (12.02 mM; Need ultrasonic and warming)
1M NaOH : 10 mg/mL (12.02 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (274 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 1M NaOH / 1M NaOH | 1 mM | 1.2019 mL | 6.0097 mL | 12.0194 mL | 30.0484 mL |
| 5 mM | 0.2404 mL | 1.2019 mL | 2.4039 mL | 6.0097 mL | |
| 10 mM | 0.1202 mL | 0.6010 mL | 1.2019 mL | 3.0048 mL |