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Cat. No.: HY-D0110B

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Fura-2 pentasodium

Fura-2 pentasodium is a cell-impermeable scaled fluorescent dye that can be used for intracellular calcium imaging. Fura-2 pentasodium has an emission wavelength of 510 nm and excitation wavelengths of 340 nm or 380 nm and the ratio of 340/380 fluorescence intensity is proportional to the intracellular Ca²⁺ level.

For research use only. We do not sell to patients.

Formula: C₂₉H₂₂N₃Na₅O₁₄
Molecular Weight:751.45

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

Fura-2 pentasodium Chemical Structure

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

In Vitro

Fura-2 itself cannot pass through the cell membrane, and various methods (such as microinjection, ATP permeabilization, scrape loading, and pH shock) can be employed to insert Fura-2 into the cytoplasm of cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

Molecular Weight 751.45
Formula C₂₉H₂₂N₃Na₅O₁₄
SMILES

O=C(O[Na])C(O1)=CN=C1C(O2)=CC(C=C3OCCOC4=C(N(CC(O[Na])=O)CC(O[Na])=O)C=CC(C)=C4)=C2C=C3N(CC(O[Na])=O)CC(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Roe MW, et al. Assessment of Fura-2 for measurements of cytosolic free calcium. Cell Calcium. 1990 Feb-Mar;11(2-3):63-73. [Content Brief]

[2]. James G. McCormack, et al. Studies on mitochondrial Ca2+-transport and matrix Ca2+ using fura-2-loaded rat heart mitochondria. Biochim Biophys Acta. 1989 Mar 23;973(3):420-7. [Content Brief]

Calculators

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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