Rhod-5N
Rhod-5N is a cell-impermeant calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd2+ is higher than other interfering cations ((Na+, K+, Mg2+, Ca2+, Zn2+) except Pb2+), and the detection limit is 3.1 μg/L.
For research use only. We do not sell to patients.
- CAS No.: 888229-31-0
- Formula: C39H36K3N5O13
- Molecular Weight:900.02
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Rhod-5N can be introduced into cells using methods such as patch clamping and microinjection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 888229-31-0
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Molecular Weight 900.02
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Formula C39H36K3N5O13
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SMILES
CN(C1=CC2=C(C=C1)C(C(C=C3)=CC(OCCOC(C=C4[N+]([O-])=O)=C(C=C4)N(CC(O[K])=O)CC(O[K])=O)=C3N(CC(O[K])=O)CC([O-])=O)=C(C=CC(N(C)C)=C5)C5=[O+]2)C
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Ca2+ Staining Technique
Ca2+ staining is an experimental technique that utilizes specific fluorescent probes (such as Fluo-4 AM, Fura-2, etc.) to qualitatively or quantitatively detect dynamic changes in intracellular Ca2+ concentrations; this is achieved by monitoring the changes in fluorescent signals generated when these probes bind to free intracellular calcium ions. The underlying principle relies primarily on the presence of chelating groups within the probe's molecular structure that possess high affinity for calcium ions.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Rhod-5N Related Classifications
HY-136894 Related Classifications
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