888229-31-0
Chemical Structure
Rhod-5N
- CAS. Nr.: 888229-31-0
- Formula:C39H36K3N5O13
- Molecular Weight:900.02
IUPAC Name: potassium 2,2'-((2-(2-(2-(bis(carboxylatomethyl)amino)-5-(3,6-bis(dimethylamino)xanthylium-9-yl)phenoxy)ethoxy)-4-nitrophenyl)azanediyl)diacetate
InChIKey: JLZGTFWLMRDWKD-UHFFFAOYSA-K
SMILES: CN(C1=CC2=C(C=C1)C(C(C=C3)=CC(OCCOC(C=C4[N+]([O-])=O)=C(C=C4)N(CC(O[K])=O)CC(O[K])=O)=C3N(CC(O[K])=O)CC([O-])=O)=C(C=CC(N(C)C)=C5)C5=[O+]2)C
Biological Activity: Rhod-5N is a cell-impermeant calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd2+ is higher than other interfering cations ((Na+, K+, Mg2+, Ca2+, Zn2+) except Pb2+), and the detection limit is 3.1 μg/L[1][2].
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Rhod-5N | Rhod-5N is a cell-impermeant calcium-binding fluorescent dye composed of a BAPTA chelating group and a rhodamine fluorophore. Rhod-5N is often added to MOPS buffer to complex and indicate cation content. The selectivity of Rhod-5N for Cd2+ is higher than other interfering cations ((Na+, K+, Mg2+, Ca2+, Zn2+) except Pb2+), and the detection limit is 3.1 μg/L. | |||||||||||||||||||||
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References
- [1]. Soibinet M, Souchon V, Leray I, et al. Rhod-5N as a fluorescent molecular sensor of cadmium (II) ion[J]. Journal of fluorescence, 2008, 18: 1077-1082.
- [2]. McNamara C J, Perry Iv T D, Bearce K, et al. Measurement of limestone biodeterioration using the Ca2+ binding fluorochrome Rhod-5N[J]. Journal of microbiological methods, 2005, 61(2): 245-250.
