Fura-2 AM (solution)  (Synonyms: Fura-2 Acetoxymethyl ester (solution))

Fura-2 AM (solution) (Fura-2 Acetoxymethyl ester (solution)) is a membrane permeable, intracellular, UV light-excitable and ratiometric fluorescent Ca²⁺ (calcium ion) indicator. Fura-2 AM crosses cell membranes and is converted to Fura-2 (HY-D0110A) via cellular esterases. Fura-2 AM can be used to detect calcium in cells.
Solvent and concentration: DMSO: 1 mM

Guide (The following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by intracellular esterases to generate free Fura-2 acid.
1. Aliquot 2 mL of culture medium into a conical tube and warm it to 37°C. Add 2 μL of Fura-2 AM stock solution to prepare a 1 μM Fura-2 AM solution. Vortex vigorously for 1 min.
2. Transfer the loading solution into a tissue culture dish, and move the cell-containing coverslip into the dish.
3. Incubate neurons at 37°C in a dark incubator for 30 min. Accurately record the incubation time.
4. Prepare a new culture dish containing 2 mL tissue culture medium without Fura-2 AM. Remove the coverslip from the loading solution and transfer it into the new dish.
5. Mount the coverslip onto the imaging chamber.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

1001.85

C₄₄H₄₇N₃O₂₄

108964-32-5

505

336

O=C(C1=CN=C(C2=CC3=CC(OCCOC4=CC(C)=CC=C4N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)=C(N(CC(OCOC(C)=O)=O)CC(OCOC(C)=O)=O)C=C3O2)O1)OCOC(C)=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Odmara L Barreto-Chang, et al. Calcium imaging of cortical neurons using Fura-2 AM. J Vis Exp. 2009 Jan 19;(23):1067.  [Content Brief]