Fura-2 AM (solution)

Guide (The following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Fura-2 AM diffuses across the cell membrane and is de-esterified by intracellular esterases to generate free Fura-2 acid.
1. Aliquot 2 mL of culture medium into a conical tube and warm it to 37°C. Add 2 μL of Fura-2 AM stock solution to prepare a 1 μM Fura-2 AM solution. Vortex vigorously for 1 min.
2. Transfer the loading solution into a tissue culture dish, and move the cell-containing coverslip into the dish.
3. Incubate neurons at 37°C in a dark incubator for 30 min. Accurately record the incubation time.
4. Prepare a new culture dish containing 2 mL tissue culture medium without Fura-2 AM. Remove the coverslip from the loading solution and transfer it into the new dish.
5. Mount the coverslip onto the imaging chamber.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.