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  4. TSQ

TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λmax ~470 nm). TSQ can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence (Ex/Em = 334/495 nm).

For research use only. We do not sell to patients.

CAS No. : 109628-27-5

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Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
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Based on 5 publication(s) in Google Scholar

Other Forms of TSQ:

TSQ Related Antibodies

Top Publications Citing Use of Products

    TSQ purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 16;23(1):447.  [Abstract]

    Intracellular Zn2+ ions detection by TSQ (0.1 M; 30 min). The results showed that the intracellular Zn2+ ion content in the nanoparticle groups was significantly higher than that in the control group, with the siRNAPD−L1@HA-ZIF-8 group exhibiting the highest intracellular Zn2+ ion content.

    TSQ purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Apr 18:113754.  [Abstract]

    Intracellular Zn2+ release over time detected using TSQ (HaCaT cells, 30 μM, 30 min at 37 °C) zinc probes (white arrow: Zn2+ fluorescence signal).

    TSQ purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Jun 18:e2501009.  [Abstract]

    TSQ intracellular Zn2+ probe detection of temporal changes in Zn2+ release from ZnO and H-ZnO in cells. White arrow indicates fluorescence signal of intracellular zinc ion release. To further clarify intracellular Zn2+ release, the TSQ (30 µM; 30 min) probe was used to detect fluorescence in L929 cells. After 15 min of ZnO treatment, intracellular Zn2+ levels significantly increased and continued to rise over time.

    TSQ purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2025 Apr 8:S1742-7061(25)00257-0.  [Abstract]

    Representative images of TSQ (10 μM; 30 min), a fluorescent Zn2+ probe, in Caco-2 cells. The results showed that Zn and I3C combined treatment significantly increased blue fluorescence intensity.

    • Biological Activity

    • Purity & Documentation

    • References

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    Description

    TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λmax ~470 nm). TSQ can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence (Ex/Em = 334/495 nm)[1].

    In Vitro

    Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
    1. Solution preparation[4]
    1.1 Preparation of stock solution
    Solvent: DMSO
    Concentration: 1-30 mM (optimized according to the experiment).
    Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
    1.2 Preparation of working solution
    Dilute to 1-30 μM with DPBS or other buffers.
    Note: The working solution should be prepared and used immediately. Keep it away from light.

    2. Cell staining
    2.1 Cells are grown on glass coverslips coated with Collagen, rat tail (HY-NP010).
    2.2 Rinsed three times with Dulbecco's phosphate buffered saline.
    2.3 30 μM TSQ in DPBS, mix well and incubate at 37°C for 30 minutes.
    2.4 After the stained cells are rinsed three times with DPBS.
    2.5 Fluorescence microscopy detection (Ex/Em = 334/495 nm).

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    TSQ Related Antibodies
    Clinical Trial
    Molecular Weight

    328.39

    Formula

    C17H16N2O3S
    CAS No.
    Appearance

    Solid

    Color

    Light brown to brown

    Emission (Em)

    495
    Excitation (Ex)

    334
    SMILES

    O=S(C1=CC=C(C)C=C1)(NC2=C3N=CC=CC3=CC(OC)=C2)=O
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage

    4°C, protect from light

    *In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (304.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.0452 mL 15.2258 mL 30.4516 mL
    5 mM 0.6090 mL 3.0452 mL 6.0903 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
    ×
    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

    ×
    Volume (final)

    V2

    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Calculation results:
    Working solution concentration: mg/mL
    Purity & Documentation
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    DMSO 1 mM 3.0452 mL 15.2258 mL 30.4516 mL 76.1290 mL
    5 mM 0.6090 mL 3.0452 mL 6.0903 mL 15.2258 mL
    10 mM 0.3045 mL 1.5226 mL 3.0452 mL 7.6129 mL
    15 mM 0.2030 mL 1.0151 mL 2.0301 mL 5.0753 mL
    20 mM 0.1523 mL 0.7613 mL 1.5226 mL 3.8064 mL
    25 mM 0.1218 mL 0.6090 mL 1.2181 mL 3.0452 mL
    30 mM 0.1015 mL 0.5075 mL 1.0151 mL 2.5376 mL
    40 mM 0.0761 mL 0.3806 mL 0.7613 mL 1.9032 mL
    50 mM 0.0609 mL 0.3045 mL 0.6090 mL 1.5226 mL
    60 mM 0.0508 mL 0.2538 mL 0.5075 mL 1.2688 mL
    80 mM 0.0381 mL 0.1903 mL 0.3806 mL 0.9516 mL
    100 mM 0.0305 mL 0.1523 mL 0.3045 mL 0.7613 mL
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    TSQ Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    TSQ109628-27-5Fluorescent DyezincInhibitorinhibitorinhibit

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