TSQ
Based on 5 publication(s) in Google Scholar
TSQ is a cytosolic zinc ion fluorescence probe that is membrane permeable and can be used for intracellular imaging of zinc proteins (λmax ~470 nm). TSQ can combine with Zn2+ in the presence of Ca2+ and Mg2+ to produce blue fluorescence (Ex/Em = 334/495 nm).
For research use only. We do not sell to patients.
- Purity: 99.50%
- CAS No.: 109628-27-5
- Formula: C17H16N2O3S
- Molecular Weight:328.39
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications Citing Use of MedChemExpress (MCE) TSQ
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
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Cell Imaging/Staining
Biological Activity
Guide (The following is our recommended protocol. This protocol is for reference only and should be modified according to your specific needs).
1. Solution preparation[4]
1.1 Preparation of stock solution
Solvent: DMSO
Concentration: 1-30 mM (optimized according to the experiment).
Storage: Store at -20°C or -80°C in dark after aliquoting. Avoid repeated freezing and thawing.
1.2 Preparation of working solution
Dilute to 1-30 μM with DPBS or other buffers.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells are grown on glass coverslips coated with Collagen, rat tail (HY-NP010).
2.2 Rinsed three times with Dulbecco's phosphate buffered saline.
2.3 30 μM TSQ in DPBS, mix well and incubate at 37°C for 30 minutes.
2.4 After the stained cells are rinsed three times with DPBS.
2.5 Fluorescence microscopy detection (Ex/Em = 334/495 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
495
334
Chemical Information
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CAS No. 109628-27-5
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Appearance Solid
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Molecular Weight 328.39
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Formula C17H16N2O3S
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Color Light brown to brown
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SMILES
O=S(C1=CC=C(C)C=C1)(NC2=C3N=CC=CC3=CC(OC)=C2)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Publications (5)
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Journal Impact Factor
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Most Recent
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J Nanobiotechnology
An immune activator encapsulating PD-L1 siRNA for augmented immune checkpoint blockade immunotherapy through Zn2+ overload triggered pyroptosis. [Abstract]2025 Jun 16;23(1):447. PMID: 40524159
TSQ purchased from MedChemExpress. Usage Cited in: J Nanobiotechnology. 2025 Jun 16;23(1):447. [Abstract]
Intracellular Zn2+ ions detection by TSQ (0.1 M; 30 min). The results showed that the intracellular Zn2+ ion content in the nanoparticle groups was significantly higher than that in the control group, with the siRNAPD−L1@HA-ZIF-8 group exhibiting the highest intracellular Zn2+ ion content.
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J Control Release
Biomimetic hyaluronic acid-stabilized zinc oxide nanoparticles in acne treatment: A preclinical and clinical approach. [Abstract]2025 Apr 18:113754. PMID: 40254135
TSQ purchased from MedChemExpress. Usage Cited in: J Control Release. 2025 Apr 18:113754. [Abstract]
Intracellular Zn2+ release over time detected using TSQ (HaCaT cells, 30 μM, 30 min at 37 °C) zinc probes (white arrow: Zn2+ fluorescence signal).
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Adv Healthc Mater
Enhanced Xanthium-Inspired ZnO for Precision Antibacterial Therapy and Regeneration of Infected Wounds. [Abstract]2025 Jun 18:e2501009. PMID: 40531599
TSQ purchased from MedChemExpress. Usage Cited in: Adv Healthc Mater. 2025 Jun 18:e2501009. [Abstract]
TSQ intracellular Zn2+ probe detection of temporal changes in Zn2+ release from ZnO and H-ZnO in cells. White arrow indicates fluorescence signal of intracellular zinc ion release. To further clarify intracellular Zn2+ release, the TSQ (30 µM; 30 min) probe was used to detect fluorescence in L929 cells. After 15 min of ZnO treatment, intracellular Zn2+ levels significantly increased and continued to rise over time.
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Acta Biomater
Engineered probiotics remodel the intestinal epithelial barrier and enhance bacteriotherapy for inflammatory bowel diseases. [Abstract]2025 Apr 8:S1742-7061(25)00257-0. PMID: 40210182
TSQ purchased from MedChemExpress. Usage Cited in: Acta Biomater. 2025 Apr 8:S1742-7061(25)00257-0. [Abstract]
Representative images of TSQ (10 μM; 30 min), a fluorescent Zn2+ probe, in Caco-2 cells. The results showed that Zn and I3C combined treatment significantly increased blue fluorescence intensity.
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Solvent & Solubility
DMSO : ≥ 100 mg/mL (304.52 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.0452 mL | 15.2258 mL | 30.4516 mL | 76.1290 mL |
| 5 mM | 0.6090 mL | 3.0452 mL | 6.0903 mL | 15.2258 mL | |
| 10 mM | 0.3045 mL | 1.5226 mL | 3.0452 mL | 7.6129 mL | |
| 15 mM | 0.2030 mL | 1.0151 mL | 2.0301 mL | 5.0753 mL | |
| 20 mM | 0.1523 mL | 0.7613 mL | 1.5226 mL | 3.8064 mL | |
| 25 mM | 0.1218 mL | 0.6090 mL | 1.2181 mL | 3.0452 mL | |
| 30 mM | 0.1015 mL | 0.5075 mL | 1.0151 mL | 2.5376 mL | |
| 40 mM | 0.0761 mL | 0.3806 mL | 0.7613 mL | 1.9032 mL | |
| 50 mM | 0.0609 mL | 0.3045 mL | 0.6090 mL | 1.5226 mL | |
| 60 mM | 0.0508 mL | 0.2538 mL | 0.5075 mL | 1.2688 mL | |
| 80 mM | 0.0381 mL | 0.1903 mL | 0.3806 mL | 0.9516 mL | |
| 100 mM | 0.0305 mL | 0.1523 mL | 0.3045 mL | 0.7613 mL |