Calcein (mixture of isomers)

Name: Calcein (mixture of isomers)
Brand: MedChemExpress
SKU: HY-D1249
Rating: 4.5 (1 reviews)

Cat. No.: HY-D1249: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.

For research use only. We do not sell to patients.

Calcein (mixture of isomers) Chemical Structure

CAS No. : 154071-48-4

Size	Stock	Quantity

Free Sample (0.1 - 0.2 mg)	Apply Now
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Based on 1 publication(s) in Google Scholar

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Biological Activity
Purity & Documentation
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Description

In Vitro

Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Take live cell staining as an example:
(1) Preparation of stock solution: Prepare a 10 mM stock solution with anhydrous DMSO and store it at -20℃.
(2) Preparation of working solution: Dilute with serum-free buffer (or PBS) to a final concentration of 0.1-10 μM, and prepare it freshly before use.
(3) Staining: Aspirate the old medium, add 1-10 μM Calcein working solution, and incubate at 37℃ in the dark for 15-60 min.
(4) Fluorescence detection: Wash 3 times with preheated PBS, replace with fresh medium, and then perform imaging detection.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

622.53

Formula

C₃₀H₂₆N₂O₁₃

CAS No.

154071-48-4

Appearance

Solid

Color

Yellow to orange

Emission (Em)

512

Excitation (Ex)

488

SMILES

O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=C3C=CC(O)=C5.CCN(CC(O)=O)CC(O)=O.CCN(CC(O)=O)CC(O)=O

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (160.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

1M NaOH : 10 mg/mL (16.06 mM; Need ultrasonic)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6063 mL	8.0317 mL	16.0635 mL
5 mM	0.3213 mL	1.6063 mL	3.2127 mL
10 mM	0.1606 mL	0.8032 mL	1.6063 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Select Batch:

Data Sheet (249 KB) SDS (393 KB)

COA (253 KB)

Handling Instructions (2659 KB)

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

1M NaOH / DMSO	1 mM	1.6063 mL	8.0317 mL	16.0635 mL	40.1587 mL
	5 mM	0.3213 mL	1.6063 mL	3.2127 mL	8.0317 mL
	10 mM	0.1606 mL	0.8032 mL	1.6063 mL	4.0159 mL
	15 mM	0.1071 mL	0.5354 mL	1.0709 mL	2.6772 mL
DMSO	20 mM	0.0803 mL	0.4016 mL	0.8032 mL	2.0079 mL
	25 mM	0.0643 mL	0.3213 mL	0.6425 mL	1.6063 mL
	30 mM	0.0535 mL	0.2677 mL	0.5354 mL	1.3386 mL
	40 mM	0.0402 mL	0.2008 mL	0.4016 mL	1.0040 mL
	50 mM	0.0321 mL	0.1606 mL	0.3213 mL	0.8032 mL
	60 mM	0.0268 mL	0.1339 mL	0.2677 mL	0.6693 mL
	80 mM	0.0201 mL	0.1004 mL	0.2008 mL	0.5020 mL
	100 mM	0.0161 mL	0.0803 mL	0.1606 mL	0.4016 mL