Calcein (mixture of isomers)
Based on 1 Customer Validation
Calcein (mixture of isomers), Calcein (HY-D0040) Derivative, is a fluorescent dye (Ex/Em = 495/515 nm). Calcein (mixture of isomers) can be used for the researches for live cell staining, calcium ion detection, bone fluorescence labeling and membrane permeability leakage detection.
For research use only. We do not sell to patients.
- CAS No.: 154071-48-4
- Formula: C30H26N2O13
- Molecular Weight:622.53
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
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Help & FAQs
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Methyl Orange
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Neutral Red (IND)
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HY-D0047
5-CFDA
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HY-118907R
Methyl Orange (Standard)
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HY-B1422R
9-Aminoacridine (Standard)
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
Take live cell staining as an example:
(1) Preparation of stock solution: Prepare a 10 mM stock solution with anhydrous DMSO and store it at -20℃.
(2) Preparation of working solution: Dilute with serum-free buffer (or PBS) to a final concentration of 0.1-10 μM, and prepare it freshly before use.
(3) Staining: Aspirate the old medium, add 1-10 μM Calcein working solution, and incubate at 37℃ in the dark for 15-60 min.
(4) Fluorescence detection: Wash 3 times with preheated PBS, replace with fresh medium, and then perform imaging detection.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Chemical Information
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CAS No. 154071-48-4
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Appearance Solid
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Molecular Weight 622.53
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Formula C30H26N2O13
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Color Yellow to orange
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SMILES
O=C(C1=C2C=CC=C1)OC32C4=C(C=C(O)C=C4)OC5=C3C=CC(O)=C5.CCN(CC(O)=O)CC(O)=O.CCN(CC(O)=O)CC(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 100 mg/mL (160.63 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
1M NaOH : 10 mg/mL (16.06 mM; Need ultrasonic)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (249 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| 1M NaOH / DMSO | 1 mM | 1.6063 mL | 8.0317 mL | 16.0635 mL | 40.1587 mL |
| 5 mM | 0.3213 mL | 1.6063 mL | 3.2127 mL | 8.0317 mL | |
| 10 mM | 0.1606 mL | 0.8032 mL | 1.6063 mL | 4.0159 mL | |
| 15 mM | 0.1071 mL | 0.5354 mL | 1.0709 mL | 2.6772 mL | |
| DMSO | 20 mM | 0.0803 mL | 0.4016 mL | 0.8032 mL | 2.0079 mL |
| 25 mM | 0.0643 mL | 0.3213 mL | 0.6425 mL | 1.6063 mL | |
| 30 mM | 0.0535 mL | 0.2677 mL | 0.5354 mL | 1.3386 mL | |
| 40 mM | 0.0402 mL | 0.2008 mL | 0.4016 mL | 1.0040 mL | |
| 50 mM | 0.0321 mL | 0.1606 mL | 0.3213 mL | 0.8032 mL | |
| 60 mM | 0.0268 mL | 0.1339 mL | 0.2677 mL | 0.6693 mL | |
| 80 mM | 0.0201 mL | 0.1004 mL | 0.2008 mL | 0.5020 mL | |
| 100 mM | 0.0161 mL | 0.0803 mL | 0.1606 mL | 0.4016 mL |
Calcein (mixture of isomers) Related Classifications
HY-D1249 Related Classifications
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