1. Dye Reagents
  2. Calcein

Calcein (Synonyms: Fluorexon)

Cat. No.: HY-D0040
Handling Instructions

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

For research use only. We do not sell to patients.

Calcein Chemical Structure

Calcein Chemical Structure

CAS No. : 1461-15-0

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 66 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 60 In-stock
Estimated Time of Arrival: December 31
200 mg   Get quote  
500 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 3 publication(s) in Google Scholar

Other Forms of Calcein:

Top Publications Citing Use of Products
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Description

Calcein is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium.

In Vitro

Calcein accumulates intracellularly in coelomocytes incubated in coelomic fluid or ISO-EDTA. Coelomocytes incubated in CF show an increase in the calcein fluorescence intensity compared to the control.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

622.53

Formula

C₃₀H₂₆N₂O₁₃

CAS No.
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 100 mg/mL (160.63 mM)

0.1 M NaOH : 10 mg/mL (16.06 mM; ultrasonic and adjust pH to 11 with NaOH)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.6063 mL 8.0317 mL 16.0635 mL
5 mM 0.3213 mL 1.6063 mL 3.2127 mL
10 mM 0.1606 mL 0.8032 mL 1.6063 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  2% NaHCO3 in ddH2O

    Solubility: 12.5 mg/mL (20.08 mM); Clear solution; Need ultrasonic

*All of the co-solvents are provided by MCE.
References
Cell Assay
[1]

Coelomocytes suspensions (ISO and CF) are separately incubated with calcein-AM (excitation and emission wavelength: 496 nm and 516 nm, respectively) at a final concentration of 200 nM, during 30 min, at 26°C. Calcein-AM is a nonfluorescent ABC transporter substrate whose intracellular accumulation is inversely proportional to ABC transporter activity. Intracellular esterases convert calcein-AM into the fluorescent dye calcein, which is not an ABC transporter substrate, thereby accumulating the dye inside the cell. Therefore, a high fluorescence signal indicates low ABC transporter activity whereas a low fluorescence signal indicates high activity. The fluorescence of samples is measured by flow cytometer. The experiment is repeated six times in duplicates.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

References
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Product Name:
Calcein
Cat. No.:
HY-D0040
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