Calcein (solution)
Based on 1 Customer Validation
Calcein (solution) (Fluorexon (solution)) is a fluorescent dye and self-quenching probe, used as an indicator of lipid vesicle leakage, and also as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. Calcein cannot directly cross the intact cell membrane of a living cell, unlike Calcein-AM (HY-D0041) which is cell-permeable. Calcein can also be used as a model drug for evaluating efficiency and bioavailability of drug delivery systems.
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
For research use only. We do not sell to patients.
- CAS No.: 1461-15-0
- Formula: C30H26N2O13
- Molecular Weight:622.53
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
Solvent and concentration: DMSO: 2 mM
The 1 mL volume is defined as the base specification. All larger sizes correspond to incremental volumes of this base.
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| CHO | Inhibition |
105.616 %
Compound: CALCEIN
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Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
Inhibition of sodium fluorescein uptake in OATP1B1-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
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[PMID: 23571415] |
| CHO | Inhibition |
121.275 %
Compound: CALCEIN
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Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
Inhibition of sodium fluorescein uptake in OATP1B3-transfected CHO cells at an equimolar substrate-inhibitor concentration of 10 uM
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[PMID: 23571415] |
In Vitro
Description: Calcein is a fluorescent probe with yellow green fluorescence, it can be used to determine labile iron pool and measure the Fe(III)-binding affinity with ligands[3].
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Solution preparation[3]
1.1 Preparation of working solution
Dilute to 0.5 μM with PBS or cell culture medium.
Note: The working solution should be prepared and used immediately. Keep it away from light.
2. Cell staining
2.1 Cells (106/mL) are loaded by incubation with 20 μM Fe(II) for 2 h at 37°C.
2.2 Cells are collected by centrifugation (1000 rpm; 5 min) and Calcein (0.5 μM) is directly injected into the cells via microinjection for staining (5-15 mins).
2.3 Wash cells for twice by D’hanks buffer (pH 7.4).
2.4 The fluorescence is monitored by both flow cytometry and a confocal laser scanning microscope.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1461-15-0
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Appearance Liquid (Density: 1.7±0.1 g/cm3)
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Molecular Weight 622.53
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Formula C30H26N2O13
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Color Light yellow to yellow
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SMILES
O=C1OC2(C3=C(OC4=C2C=C(CN(CC(O)=O)CC(O)=O)C(O)=C4)C=C(O)C(CN(CC(O)=O)CC(O)=O)=C3)C5=C1C=CC=C5
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Synonyms
Fluorexon (solution)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Protocol for Pharmacokinetic Study
Pharmacokinetic studies quantify how an organism handles a drug over time through absorption, distribution, metabolism, and excretion, and the core experimental readout is the concentration-time profile of parent drug and, when relevant, metabolites in biological matrices such as plasma, whole blood, urine, bile, or tissue. Pharmacokinetic analysis links dose, route, exposure, clearance, half-life, distribution, bioavailability, and systemic exposure to drug efficacy and toxicity hypotheses rather than measuring a signaling pathway directly. The literature links pharmacokinetics to drug-development phenotypes by showing that drug metabolism and pharmacokinetics influence compound progression, exposure-response interpretation, safety margins, dosing strategy, and failure risk during discovery and development. DMPK science contributes to compound optimization by integrating physicochemical properties, in vitro metabolism, transporter behavior, in vivo exposure, and pharmacodynamic contex
Purity & Documentation
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Data Sheet (274 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Marques-Santos LF, et al. ABCB1 and ABCC1-like transporters in immune system cells from sea urchins Echinometra lucunter and Echinus esculentus and oysters Crassostrea gasar and Crassostrea gigas. Fish Shellfish Immunol. 2017 Sep 5;70:195-203. [Content Brief]
[2]. Shao B, et al. Caffeic Acid Phenyl Ester (CAPE) Protects against Iron-Mediated Cellular DNA Damage through Its Strong Iron-Binding Ability and High Lipophilicity. Antioxidants (Basel). 2021 May 18;10(5):798. [Content Brief]
[3]. Maherani B, et al., Calcein release behavior from liposomal bilayer; influence of physicochemical/mechanical/structural properties of lipids. Biochimie. 2013 Nov;95(11):2018-33. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Calcein (solution) Related Classifications
HY-DY1091 Related Classifications
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