1. Metabolic Enzyme/Protease
  2. Hexokinase
  3. 2-Deoxy-D-glucose

2-Deoxy-D-glucose (Synonyms: 2-Deoxy-D-arabino-hexose; D-Arabino-2-deoxyhexose)

Cat. No.: HY-13966 Purity: >99.0%
Handling Instructions

2-Deoxy-D-glucose is a glucose analog that acts as a competitive inhibitor of glucose metabolism, inhibiting glycolysis via its actions on hexokinase.

For research use only. We do not sell to patients.

2-Deoxy-D-glucose Chemical Structure

2-Deoxy-D-glucose Chemical Structure

CAS No. : 154-17-6

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Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 66 In-stock
Estimated Time of Arrival: December 31
1 g USD 60 In-stock
Estimated Time of Arrival: December 31
5 g USD 192 In-stock
Estimated Time of Arrival: December 31
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Customer Review

Based on 7 publication(s) in Google Scholar

Top Publications Citing Use of Products

    2-Deoxy-D-glucose purchased from MCE. Usage Cited in: Oncotarget. 2017 Jul 4;8(27):44669-44681.

    Effect of 2-DG treatment on BRSK2 and other components of AKT/mTOR signaling pathway. PANC-1 cells are treated with series concentrations of 2-DG for three hours before analysis.
    • Biological Activity

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    • References

    Description

    2-Deoxy-D-glucose is a glucose analog that acts as a competitive inhibitor of glucose metabolism, inhibiting glycolysis via its actions on hexokinase.

    In Vitro

    2-Deoxy-D-glucose (2-DG, 4, 8, or 16 mM) significantly reduces the level of ATP in MCF-7 cells in a dose- and time-dependent manner that paralleles the effects of 2-DG on cell growth. The levels of phosphorylated Akt are significantly decreased, whereas the levels of phosphorylated AMPK and Sirt-1 are significantly increased in MCF-7 cells exposed to 2-Deoxy-D-glucose at 4, 8, or 16 mM for 1, 3, or 5 days in a dose- and time-dependent manner[1]. 2-DG treatment increases the levels of pentose phosphate pathway (PPP) metabolites and augments the generation of NADPH by glucose-6-phosphate dehydrogenase. An increase in NADPH and upregulation of glutathione synthetase expression resultes in the increase in the reduced form of glutathione by 2-DG in NB4 cells[3].

    In Vivo

    2-Deoxy-D-glucose (0.03%, w/w) causes a 7% decrease in final weight that is statistically significant, and delayes the appearance of palpable mammary carcinomas[1]. 2-Deoxy-D-glucose (3 mmol/kg, i.v.) is decreased in a dose-dependent manner by insulin in rat muscle[2].

    Clinical Trial
    Storage

    4°C, protect from light

    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 51 mg/mL (310.67 mM)

    H2O : ≥ 24 mg/mL (146.20 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 6.0916 mL 30.4581 mL 60.9162 mL
    5 mM 1.2183 mL 6.0916 mL 12.1832 mL
    10 mM 0.6092 mL 3.0458 mL 6.0916 mL
    *Please refer to the solubility information to select the appropriate solvent.
    References
    Cell Assay
    [1]

    The effect of 2-DG on cell growth is determined by evaluating the number of adherent cells. Briefly, MCF-7 cells are plated at 3×104 cells per well in flat-bottomed 96-well plates in 100 μL of culture medium under the culture conditions. After 24 hours, cells are fed with fresh medium including 2-Deoxy-D-glucose at doses of 0, 4, 8, or 16 mM. At days 1, 3, and 5 after 2-Deoxy-D-glucose exposure, cells are fixed with 1% glutaraldehyde, replaced with PBS, and stored at 4°C. At the end of an experiment, all of the plates are stained with 0.02% aqueous crystal violet for 30 minutes and rinsed with deionized water. After redissolving the bound crystal violet in 70% ethanol, the absorbance is determined at 590 nm using a SPECTRA MAX PLUS Microplate Spectrophotometer System.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    Animal Administration
    [1]

    At 21 days of age, rats are injected with 50 mg 1-methyl-1-nitrosourea per kilogram of body weight (i.p.). Rats are housed two per cage in solid-bottomed polycarbonate cages equipped with a food cup. Six days following carcinogen injection, all rats are randomized into one of three groups, 30 rats per group, and are fed ad libitum AIN-93G diet containing 0.0%, 0.02%, or 0.03% (w/w) 2-Deoxy-D-glucose (2-DG) for 5 weeks. Animal rooms are maintained at 22±1°C with 50% relative humidity and a 12-hour light/12-hour dark cycle. Rats are weighed thrice per week and are palpated for detection of mammary tumors twice per week starting from 19 days postcarcinogen.

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.

    References
    Molecular Weight

    164.16

    Formula

    C₆H₁₂O₅

    CAS No.

    154-17-6

    SMILES
    Shipping

    Room temperature in continental US; may vary elsewhere

    Purity: >99.0%

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    Product Name:
    2-Deoxy-D-glucose
    Cat. No.:
    HY-13966
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    2-Deoxy-D-glucose

    Cat. No.: HY-13966