1. Academic Validation
  2. Glycogen metabolism reprogramming promotes inflammation in coal dust-exposed lung

Glycogen metabolism reprogramming promotes inflammation in coal dust-exposed lung

  • Ecotoxicol Environ Saf. 2022 Sep 1;242:113913. doi: 10.1016/j.ecoenv.2022.113913.
Wenyang Wang 1 Min Mu 2 Yuanjie Zou 3 Songsong Deng 4 Yuting Lu 3 Qinglong Li 3 Zeyu Li 3 Huihui Tao 2 Yun Wang 5 Xinrong Tao 6
Affiliations

Affiliations

  • 1 Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, China; Anhui Province Engineering Laboratory of Occupational Health and Safety, China; School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology, China.
  • 2 Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, China; Anhui Province Engineering Laboratory of Occupational Health and Safety, China; School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology, China; Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, China.
  • 3 School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology, China.
  • 4 Department of Clinical Laboratory, Chaoyang Hospital, Huainan, China.
  • 5 School of Bioengineering, Huainan Normal University, Huainan 232038, China.
  • 6 Key Laboratory of Industrial Dust Control and Occupational Health of the Ministry of Education, Anhui University of Science and Technology, China; Anhui Province Engineering Laboratory of Occupational Health and Safety, China; School of Medicine, Department of Medical Frontier Experimental Center, Anhui University of Science and Technology, China; Key Laboratory of Industrial Dust Deep Reduction and Occupational Health and Safety of Anhui Higher Education Institutes, Anhui University of Science and Technology, China. Electronic address: [email protected].
Abstract

Long-term coal dust exposure triggers complex inflammatory processes in the coal workers' pneumoconiosis (CWP) lungs. The progress of the inflammation is reported to be affected by disordered cell metabolism. However, the changes in the metabolic reprogramming associated with the pulmonary inflammation induced by the coal dust particles are unknown. Herein, we show that coal dust exposure causes glycogen accumulation and the reprogramming of glucose metabolism in the CWP lung. The glycogen accumulation caused by coal dust is mainly due to macrophages, which reprogram glycogen metabolism and trigger an inflammatory response. In addition, 2-deoxy-D-glucose (2-DG) reduced glycogen content in macrophages, which was accompanied by mitigated inflammation and restrained NF-κB activation. Accordingly, we have pinpointed a novel and crucial metabolic pathway that is an essential regulator of the inflammatory phenotype of coal dust-exposed macrophages. These results shed LIGHT on new ways to regulate CWP inflammation.

Keywords

2-DG; Coal workers’ pneumoconiosis; Glycogen metabolism; Pulmonary inflammation; scMetabolism; scRNA-seq.

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