2. Neuronal Signaling Autophagy Metabolic Enzyme/Protease
  3. Oxidative Phosphorylation PINK1/Parkin Mitochondrial Metabolism
  4. FCCP

FCCP  (Synonyms: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)

Cat. No.: HY-100410 Purity: 99.69%
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FCCP is an uncoupler of oxidative phosphorylation (OXPHOS) in mitochondria. FCCP induces activation of PINK1 leading to Parkin Ser65 phosphorylation.

For research use only. We do not sell to patients.

CAS No. : 370-86-5

Size Price Stock Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO
ready for reconstitution
 In-stock
Solution
10 mM * 1 mL in DMSO In-stock
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Customer Review

Based on 106 publication(s) in Google Scholar

Other Forms of FCCP:

FCCP Related Antibodies

Top Publications Citing Use of Products

106 Publications Citing Use of MCE FCCP

Bio/Physico-chemical Assay
Cell Imaging/Staining
Flow Cytometry
IF
WB
Cell Proliferation/Viability Assay

    FCCP purchased from MedChemExpress. Usage Cited in: Nature. 2025 Mar;639(8055):765-775.  [Abstract]

    OCR of neuroendocrine (n=4) and non-neuroendocrine (n=2) SCLC cell lines after FCCP (1.5 μM, 40 min) treatment (n=6 technical replicates for each cell line).

    FCCP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 30;16(1):6996.  [Abstract]

    The mitophagy index of shRNA- or non-transduced 528NS and HOG-GSCs treated for 3 days with or without MitoTEMPOL (100 nM) on day 4 after shRNA transduction. FCCP (30 μM) and RA were used as positive controls.

    FCCP purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 28;12(1):49.  [Abstract]

    Immunofluorescence staining to show the CTSK expression level in osteoclasts that were treated with oligomycin A (10 nmol/L), rotenone (100 nmol/L), FCCP (1 μmol/L), 2DG (1 μmol/L) or 3BrPA (1 μmol/L) for 6 h (scale bar, 100 µm).

    FCCP purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 28;12(1):49.  [Abstract]

    Immunoblots of the pre-proenzyme and active CTSK levels in osteoclasts treated with 2DG (1 μmol/L), FCCP (1 μmol/L), rotenone (100 nmol/L) or 3-BrPA (1 μmol/L) for 10 h. The MMP-9 and CTSB expression levels were also detected. DMSO-treated osteoclasts were used as controls.

    FCCP purchased from MedChemExpress. Usage Cited in: Cancer Res. 2023 Dec 15;83(24):4063-4079.  [Abstract]

    Viability of Glut5-overexpressing and control cells cultured for 24 hours under different conditions in the presence of Rotenone or FCCP (10 μmol/L).

    FCCP purchased from MedChemExpress. Usage Cited in: Nat Commun. 2022 May 13;13(1):2673.  [Abstract]

    Flow cytometry analysis of WT and Myo19 KO cells stained with 100 nM TMRM for 20 min. 5 μM FCCP treatment for 10 min was used as positive.

    • Biological Activity

    • Protocol

    • Purity & Documentation

    • References

    • Customer Review

    Description

    FCCP is an uncoupler of oxidative phosphorylation (OXPHOS) in mitochondria. FCCP induces activation of PINK1 leading to Parkin Ser65 phosphorylation[1].

    Cellular Effect
    Cell Line Type Value Description References
    T47D IC50
    0.31 μM
    Compound: FCCP
    Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay
    Inhibition of 1, 10-phenanthroline-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay
    		[PMID: 20929261]
    T47D IC50
    0.51 μM
    Compound: FCCP
    Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay
    Inhibition of hypoxia-induced HIF1 activation in human T47D cells by HRE3-TK-luciferase reporter gene assay
    		[PMID: 20929261]
    In Vitro

    FCCP (5 μM) results in a concentration-dependent decrease in Aβ and APPsβ production in K695sw cells. FCCP inhibits processing of wild-type APP. FCCP does not alter cellular ATP levels at any of the concentrations measured. The effects of FCCP on APP catabolism are independent of secondary effects on oxidative phosphorylation or the result of reduced cell viability in K695sw cells. FCCP (5 μM or 500 nM), baf A1, and NH4Cl induce changes in Tf-Tx and Tf-F cellular fluorescence in K695 cells[1].
    FCCP (200 nM) protects and enhances the follicle integrity in cat ovarian tissue during short-term in vitro culture. But FCCP does not appear to exert a beneficial or detrimental effect during ovarian tissue cryopreservation[2].

    MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

    FCCP Related Antibodies
    Molecular Weight

    254.17

    Formula

    C10H5F3N4O
    CAS No.
    Appearance

    Solid

    Color

    Light yellow to yellow

    SMILES

    N#C/C(C#N)=N/NC1=CC=C(OC(F)(F)F)C=C1
    Shipping

    Room temperature in continental US; may vary elsewhere.
    Storage
    Powder -20°C 3 years
    4°C 2 years
    In solvent -80°C 1 year
    -20°C 6 months
    Solvent & Solubility
    In Vitro: 

    DMSO : ≥ 100 mg/mL (393.44 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

    Ethanol : ≥ 33.3 mg/mL (131.01 mM)

    *"≥" means soluble, but saturation unknown.

    Preparing
    Stock Solutions
    Concentration Solvent Mass 1 mg 5 mg 10 mg
    1 mM 3.9344 mL 19.6719 mL 39.3437 mL
    5 mM 0.7869 mL 3.9344 mL 7.8687 mL
    View the Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    • Molarity Calculator

    • Dilution Calculator

    Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

    Mass
    =
    Concentration
    ×
    Volume
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    Molecular Weight *

    Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

    This equation is commonly abbreviated as: C1V1 = C2V2

    Concentration (start)

    C1

    ×
    Volume (start)

    V1

    =
    Concentration (final)

    C2

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    Volume (final)

    V2

    In Vivo:

    Select the appropriate dissolution method based on your experimental animal and administration route.

    For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
    To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
    The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

    • Protocol 1

      Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% Saline

      Solubility: 2.5 mg/mL (9.84 mM); Suspended solution; Need ultrasonic and warming

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

      Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
    • Protocol 2

      Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in Saline)

      Solubility: 2.5 mg/mL (9.84 mM); Suspended solution; Need ultrasonic and warming

      This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

      Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

      Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
    In Vivo Dissolution Calculator
    Please enter the basic information of animal experiments:

    Dosage

    mg/kg

    Animal weight
    (per animal)

    g

    Dosing volume
    (per animal)

    μL

    Number of animals

    Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
    Please enter your animal formula composition:
    %
    DMSO +
    % 
    +
    %
    Tween-80 +
    %
    Saline
    Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
    The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
    Calculation results:
    Working solution concentration: mg/mL
    Method for preparing stock solution: mg drug dissolved in μL  DMSO (Stock solution concentration: mg/mL).
    The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).
    Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.
     If the continuous dosing period exceeds half a month, please choose this protocol carefully.
    Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
    Purity & Documentation

    Purity: 99.69%

    SDS (420 KB)

    COA (244 KB) HNMR (245 KB) RP-HPLC (266 KB) LCMS (91 KB)

    Handling Instructions (2659 KB)
    References
    Cell Assay
    [1]

    K695sw cells are maintained and exposed to vehicle or various concentrations of FCCP as mentioned above with the exception that cells are plated at a density of 20,000 cells per well in 96-well plates. Twenty-four hours after plating, cells are exposed to various treatments in Dulbecco’s modified Eagle’s medium supplemented with sodium pyruvate (1 mM). At the same time as drug exposures, YO-PRO (4 μM) is added to each well, and its uptake is quantified every 30 min for 1 day at 37°C using a Cytofluor 2350 fluorometric plate reader. As a positive control, all wells are exposed to 0.1% Triton X-100 at the end of the experiment[1].

    MCE has not independently confirmed the accuracy of these methods. They are for reference only.
    References

    Complete Stock Solution Preparation Table

    * Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
    Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

    Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
    Ethanol / DMSO 1 mM 3.9344 mL 19.6719 mL 39.3437 mL 98.3594 mL
    5 mM 0.7869 mL 3.9344 mL 7.8687 mL 19.6719 mL
    10 mM 0.3934 mL 1.9672 mL 3.9344 mL 9.8359 mL
    15 mM 0.2623 mL 1.3115 mL 2.6229 mL 6.5573 mL
    20 mM 0.1967 mL 0.9836 mL 1.9672 mL 4.9180 mL
    25 mM 0.1574 mL 0.7869 mL 1.5737 mL 3.9344 mL
    30 mM 0.1311 mL 0.6557 mL 1.3115 mL 3.2786 mL
    40 mM 0.0984 mL 0.4918 mL 0.9836 mL 2.4590 mL
    50 mM 0.0787 mL 0.3934 mL 0.7869 mL 1.9672 mL
    60 mM 0.0656 mL 0.3279 mL 0.6557 mL 1.6393 mL
    80 mM 0.0492 mL 0.2459 mL 0.4918 mL 1.2295 mL
    100 mM 0.0393 mL 0.1967 mL 0.3934 mL 0.9836 mL
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    FCCP Related Classifications

    Help & FAQs
    • Do most proteins show cross-species activity?

      Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

    Keywords:

    FCCP370-86-5Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazoneOxidative PhosphorylationPINK1/ParkinMitochondrial MetabolismPTEN-induced kinase 1/ParkinInhibitorinhibitorinhibit

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