1. Metabolic Enzyme/Protease
  2. Mitochondrial Metabolism

FCCP (Synonyms: Carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone)

Cat. No.: HY-100410 Purity: >98.0%
Handling Instructions

FCCP is a mitochondrial uncoupling agent, used in cancer research.

For research use only. We do not sell to patients.
FCCP Chemical Structure

FCCP Chemical Structure

CAS No. : 370-86-5

Size Price Stock Quantity
Free Sample (0.5-1 mg)   Apply now  
10 mM * 1 mL in DMSO USD 73 In-stock
10 mg USD 66 In-stock
50 mg USD 228 In-stock
100 mg USD 384 In-stock
200 mg   Get quote  
500 mg   Get quote  

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Customer Review

  • Biological Activity

  • Protocol

  • Technical Information

  • Purity & Documentation

  • References


FCCP is a mitochondrial uncoupling agent, used in cancer research.

In Vitro

FCCP (5 μM) results in a concentration-dependent decrease in Aβ and APPsβ production in K695sw cells. FCCP inhibits processing of wild-type APP. FCCP does not alter cellular ATP levels at any of the concentrations measured. The effects of FCCP on APP catabolism are independent of secondary effects on oxidative phosphorylation or the result of reduced cell viability. FCCP (5 μM or 500 nM), baf A1, and NH4Cl induce changes in Tf-Tx and Tf-F cellular fluorescence in K695 cells[1]. FCCP (200 nM) protects and enhances the follicle integrity in cat ovarian tissue during short-term in vitro culture. But FCCP does not appear to exert a beneficial or detrimental effect during ovarian tissue cryopreservation[2].

Preparing Stock Solutions
Concentration Volume Mass 1 mg 5 mg 10 mg
1 mM 3.9344 mL 19.6719 mL 39.3437 mL
5 mM 0.7869 mL 3.9344 mL 7.8687 mL
10 mM 0.3934 mL 1.9672 mL 3.9344 mL
Please refer to the solubility information to select the appropriate solvent.
Cell Assay

FCCP is dissolved in 0.05% ethanol.

K695sw cells are maintained and exposed to vehicle or various concentrations of FCCP as mentioned above with the exception that cells are plated at a density of 20,000 cells per well in 96-well plates. Twenty-four hours after plating, cells are exposed to various treatments in Dulbecco’s modified Eagle’s medium supplemented with sodium pyruvate (1 mM). At the same time as drug exposures, YO-PRO (4 μM) is added to each well, and its uptake is quantified every 30 min for 1 day at 37°C using a Cytofluor 2350 fluorometric plate reader. As a positive control, all wells are exposed to 0.1% Triton X-100 at the end of the experiment. MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight








Powder -20°C 3 years
  4°C 2 years
In solvent -80°C 6 months
  -20°C 1 month

Room temperature in continental US; may vary elsewhere

Solvent & Solubility

DMSO: ≥ 2.6 mg/mL

* "<1 mg/mL" means slightly soluble or insoluble. "≥" means soluble, but saturation unknown.

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