Calcein (tetraethyl ester)
Based on 1 Customer Validation
Calcein tetraethyl ester is a tetraethylated derivative of calcein, with its core function serving as a live cell-specific fluorescent tracer. Calcein tetraethyl ester can diffuse between human periodontal ligament cells via gap junctions, thus enabling the evaluation of gap junction intercellular communication function, and its diffusion is enhanced under hydrogen peroxide induction. Calcein tetraethyl ester can be applied in the fields of cell biology and fluorescent labeling.
For research use only. We do not sell to patients.
- Purity: 99.52%
- CAS No.: 1170856-93-5
- Formula: C38H42N2O13
- Molecular Weight:734.75
-
Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
Calcein tetraethyl ester (5 μM; 30 min) assays show that hydrogen peroxide treatment significantly increases the number of recipient cells labeled by Calcein tetraethyl ester, confirming that the expression level of Cx43 is positively correlated with gap junction intercellular communication[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
515
489
Chemical Information
-
CAS No. 1170856-93-5
-
Appearance Solid
-
Molecular Weight 734.75
-
Formula C38H42N2O13
-
Color Light yellow to yellow
-
SMILES
O=C1OC2(C3=C(OC4=C2C=CC(O)=C4CN(CC(OCC)=O)CC(OCC)=O)C(CN(CC(OCC)=O)CC(OCC)=O)=C(O)C=C3)C5=C1C=CC=C5
-
Synonyms
Fluorexon tetraethyl ester
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 25 mg/mL (34.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (276 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3610 mL | 6.8050 mL | 13.6101 mL | 34.0252 mL |
| 5 mM | 0.2722 mL | 1.3610 mL | 2.7220 mL | 6.8050 mL | |
| 10 mM | 0.1361 mL | 0.6805 mL | 1.3610 mL | 3.4025 mL | |
| 15 mM | 0.0907 mL | 0.4537 mL | 0.9073 mL | 2.2683 mL | |
| 20 mM | 0.0681 mL | 0.3403 mL | 0.6805 mL | 1.7013 mL | |
| 25 mM | 0.0544 mL | 0.2722 mL | 0.5444 mL | 1.3610 mL | |
| 30 mM | 0.0454 mL | 0.2268 mL | 0.4537 mL | 1.1342 mL |