9-Aminoacridine (Synonyms: Aminacrine)

Name: 9-Aminoacridine
Brand: MedChemExpress
SKU: HY-B1422
Rating: 5.0 (4 reviews)

Cat. No.: HY-B1422 Purity: 99.35%: Data Sheet
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9-Aminoacridine, a fluorescent probe, acts as an indicator of pH for quantitative determination of transmembrane pH gradients (inside acidic). 9-Aminoacridine is an antimicrobial. 9-Aminoacridine exerts its antimicrobial activity by interacting with specific bacterial DNA and disrupting the proton motive force in K. pneumoniae. 9-Aminoacridine is a HIV-1 inhibitor and inhibits HIV LTR transcription highly dependent on the presence and location of the amino moiety. 9-Aminoacridine inhibits virus replication in HIV-1 infected cell lines. 9-Aminoacridine is used as a Rifampin (RIF; HY-B0272) adjuvant for the multidrug-resistant K. pneumoniae infections.

For research use only. We do not sell to patients.

9-Aminoacridine Chemical Structure

CAS No. : 90-45-9

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10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
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10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
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250 mg	In-stock	Estimated Time of Arrival: December 31
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Based on 4 publication(s) in Google Scholar

Other Forms of 9-Aminoacridine:

Acridin-9-amine hydrochloride hydrate In-stock
9-Aminoacridine-¹³C₆ Get quote
9-Aminoacridine (Standard) Get quote

4 Publications Citing Use of MCE 9-Aminoacridine

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HIV HIV-1 HIV-2

Biological Activity
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References
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Description

IC₅₀ & Target^[3]/sup>

HIV-1

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	GI₅₀	2 μM Compound: 9-AA	Growth inhibition of human HL60 cells Growth inhibition of human HL60 cells	[PMID: 20980148]
HT-22	EC₅₀	9.19 μM Compound: 47	Cytotoxicity against mouse HT22 cells after 24 hrs by MTT assay Cytotoxicity against mouse HT22 cells after 24 hrs by MTT assay	[PMID: 24602904]
Hepatocyte	IC₅₀	120 μM Compound: 10	Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes Inhibition of apamin-sensitive SKCa channel of guinea-pig hepatocytes	[PMID: 15225721]
L1210	IC₅₀	2600 nM Compound: 9	Evaluated for concentration which when added to cultures of L1210 leukemia cells for a period of 70 hr reduces cell numbers of 50% of control cultures Evaluated for concentration which when added to cultures of L1210 leukemia cells for a period of 70 hr reduces cell numbers of 50% of control cultures	[PMID: 3572974]
SK-OV-3	IC₅₀	2.53 μM Compound: 9AA	Cytotoxicity against human SKOV3 cells transfected with non-targeted vector assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay Cytotoxicity against human SKOV3 cells transfected with non-targeted vector assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay	[PMID: 31514018]
SK-OV-3	IC₅₀	2.57 μM Compound: 9AA	Cytotoxicity against human SKOV3 cells transfected with shRNA assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay Cytotoxicity against human SKOV3 cells transfected with shRNA assessed as reduction in cell viability incubated for 96 hrs in presence of doxycycline by MTT assay	[PMID: 31514018]
SK-OV-3	IC₅₀	2.78 μM Compound: 9AA	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 31514018]
SK-OV-3	IC₅₀	5.43 μM Compound: 9AA	Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay Cytotoxicity against human SKOV3 cells assessed as reduction in cell viability incubated for 24 hrs by MTT assay	[PMID: 31514018]
TOV112D	IC₅₀	3.15 μM Compound: 9AA	Cytotoxicity against human TOV112D cells expressing NQO2 assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human TOV112D cells expressing NQO2 assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 31514018]
TOV112D	IC₅₀	3.6 μM Compound: 9AA	Cytotoxicity against human TOV112D cells transfected with empty vector assessed as reduction in cell viability incubated for 96 hrs by MTT assay Cytotoxicity against human TOV112D cells transfected with empty vector assessed as reduction in cell viability incubated for 96 hrs by MTT assay	[PMID: 31514018]

In Vitro

9-Aminoacridine has a strong antimicrobial effect on K. pneumoniae, with MICs from 8 to 16?μg/mL^[1].
9-Aminoacridine (0-64?μg/mL) shows moderate cytotoxicity to normal (LO2, HK2, HMC3) and tumor (HepG2, 786-O, U251) human cell lines, but when used as a RIF adjuvant, the reduced dosage of 9-Aminoacridine further diminishes its toxicity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

9-Aminoacridine (Aminacrine; 15 mg/kg; subcutaneously) with a single dose decreases the abscess area in the skin subcutaneous abscess mice model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	7-week-old ICR female mice with the skin subcutaneous abscess model^[2]
Dosage:	15 mg/kg
Administration:	Subcutaneously
Result:	A single dose of 15 mg/kg 9-AA decreased the abscess area. A single dose of 15 mg/kg 9-AA or 20 mg/kg Rifampin (RIF; HY-B0272) did not significantly reduce the viable bacterial loads in the abscess. The combined use of the drugs significantly decreased the bacterial load by 3.15 log₁₀ CFU/abscess.

Molecular Weight

194.24

Formula

C₁₃H₁₀N₂

CAS No.

90-45-9

Appearance

Solid

Color

Light yellow to yellow

SMILES

NC1=C(C=CC=C2)C2=NC3=CC=CC=C31

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (514.83 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.1483 mL	25.7414 mL	51.4827 mL
5 mM	1.0297 mL	5.1483 mL	10.2965 mL
10 mM	0.5148 mL	2.5741 mL	5.1483 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

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In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 99.35%

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Data Sheet (279 KB) SDS (537 KB)

COA (252 KB) HNMR (129 KB) RP-HPLC (224 KB) LCMS (109 KB)

Handling Instructions (2659 KB)

References

[1]. S Grzesiek, et al. The 'delta pH'-probe 9-aminoacridine: response time, binding behaviour and dimerization at the membrane. Biochim Biophys Acta. 1988 Mar 3;938(3):411-24. [Content Brief]

[2]. Pengfei She, et al. Repurposing 9-Aminoacridine as an Adjuvant Enhances the Antimicrobial Effects of Rifampin against Multidrug-Resistant Klebsiella pneumonia. Microbiol Spectr. 2023 Jun 15;11(3):e0447422. [Content Brief]

[3]. Irene Guendel, et al. 9-Aminoacridine inhibition of HIV-1 Tat dependent transcription. Virol J. 2009 Jul 24:6:114. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	5.1483 mL	25.7414 mL	51.4827 mL	128.7068 mL
	5 mM	1.0297 mL	5.1483 mL	10.2965 mL	25.7414 mL
	10 mM	0.5148 mL	2.5741 mL	5.1483 mL	12.8707 mL
	15 mM	0.3432 mL	1.7161 mL	3.4322 mL	8.5805 mL
	20 mM	0.2574 mL	1.2871 mL	2.5741 mL	6.4353 mL
	25 mM	0.2059 mL	1.0297 mL	2.0593 mL	5.1483 mL
	30 mM	0.1716 mL	0.8580 mL	1.7161 mL	4.2902 mL
	40 mM	0.1287 mL	0.6435 mL	1.2871 mL	3.2177 mL
	50 mM	0.1030 mL	0.5148 mL	1.0297 mL	2.5741 mL
	60 mM	0.0858 mL	0.4290 mL	0.8580 mL	2.1451 mL
	80 mM	0.0644 mL	0.3218 mL	0.6435 mL	1.6088 mL
	100 mM	0.0515 mL	0.2574 mL	0.5148 mL	1.2871 mL

9-Aminoacridine Related Classifications

Infection

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

9-Aminoacridine90-45-9AminacrineBacterialHIVHuman immunodeficiency virusIndicatorPhpH gradientsantimicrobialK. pneumoniaHIV LTR transcriptionamino moietyvirus replicationadjuvantInhibitorinhibitorinhibit

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9-Aminoacridine (Synonyms: Aminacrine)

Customer Review

Other Forms of 9-Aminoacridine:

9-Aminoacridine Related Antibodies

4 Publications Citing Use of MCE 9-Aminoacridine

Featured Recommendations

•Related Small Molecules:

•Related Proteins:

View All HIV Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

9-Aminoacridine Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

9-Aminoacridine Related Classifications

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