Cat. No.: HY-N0053

Psoralen

(Synonyms: Ficusin)

6 Cited Publications

Customer Review

Based on 6 publication(s) in Google Scholar

Psoralen (Ficusin) is a coumarin isolated from the seeds of Fructus Psoraleae. Psoralen exhibits a wide range of biological properties, including anti-cancer, antioxidant, antidepressant, anticancer, antibacterial, and antiviral, et al.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 66-97-7
Formula: C₁₁H₆O₃
Molecular Weight:186.16

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Psoralen Chemical Structure

CAS No.: 66-97-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- Cellular Effect
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Publications (6)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

All HIV Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	>10 μM Compound: 20	Cytotoxicity against human A549 cells after 48 hrs by MTT assay Cytotoxicity against human A549 cells after 48 hrs by MTT assay	[PMID: 25710081]
B16	IC₅₀	>200 μg/mL Compound: 7	Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as melanin release after 72 hrs Inhibition of alpha-MSH-stimulated melanogenesis in mouse B16 cells assessed as melanin release after 72 hrs	[PMID: 22450129]
HCT-116	IC₅₀	45.6 μM Compound: 5	Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay Cytotoxicity against human HCT116 cells after 96 hrs by MTT assay	[PMID: 23746477]
HEK293	IC₅₀	>167 μM Compound: 3	Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay Cytotoxicity against HEK239 cells after 72 hrs by Alamar blue assay	[PMID: 20558060]
HL-60	IC₅₀	16 μM Compound: 1	Cytotoxicity against HL-60 (human fibrosarcoma) cell line; Range 0.4-16 uM Cytotoxicity against HL-60 (human fibrosarcoma) cell line; Range 0.4-16 uM	[PMID: 15837311]
HT-29	IC₅₀	41.7 μM Compound: 5	Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay Cytotoxicity against human HT-29 cells after 96 hrs by MTT assay	[PMID: 23746477]
K562	IC₅₀	>10 μM Compound: 20	Cytotoxicity against human K562 cells after 48 hrs by MTT assay Cytotoxicity against human K562 cells after 48 hrs by MTT assay	[PMID: 25710081]
K562	IC₅₀	131 μM Compound: 5	Cytotoxicity against human K562 cells after 96 hrs by MTT assay Cytotoxicity against human K562 cells after 96 hrs by MTT assay	[PMID: 23746477]
LoVo	IC₅₀	16 μM Compound: 1	Cytotoxicity against LoVo (human intestinal adenocarcinoma) cell line; Range 0.4-16 uM Cytotoxicity against LoVo (human intestinal adenocarcinoma) cell line; Range 0.4-16 uM	[PMID: 15837311]

In Vitro

Psoralen (10-500 μM; 24-48 hours) inhibits cell viability in a concentration- and time-dependent manner in L02 and?HepG2 cells. In L02 cells, Psoralen at 400 μM does not significantly change extracellular LDH levels, and 400 μM or 450 μM psoralen inhibits 50–60% of cell viability^[1].
Psoralen (150-450 μM; 24 hours) induces significant S-phase arrest in L02 cells in time- and dose-dependent manners, but it does not exhibits significant change in the cycle distribution of HepG2 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay^[1]

Cell Line:

L02 and HepG2 cells
Concentration:

10 μM, 50 μM,100 μM, 200 μM, 300 μM,400 μM,450 μM,500 μM
Incubation Time:

24 or 48 hours
Result:

Inhibited the viability of L02 and HepG2 cells mainly by suppressing cell proliferation rather than causing cell death.

Cell Cycle Analysis^[1]

Cell Line:

L02 and HepG2 cells
Concentration:

150 μM; 300 μM; 450 μM
Incubation Time:

24 or 48 hours
Result:

Induced cell S-phase arrest instead of causing cell apoptosis or death.

In Vivo

Psoralen (oral gavage; 17.5 mg/kg; 6 weeks) reduces the number of metastatic lesions and the rate of bone metastasis by 20% compared to vehicle-treated mice. It also reduces tumor infiltration and decreases the percentage of tumor cells in metastatic lesions by ~40% compared to vehicle in mice^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female nude (BALB/c nu/nu) mice^[2]
Dosage:

17.5 mg/kg
Administration:

Oral gavage; 17.5 mg/kg; 6 weeks
Result:

Inhibited metastasis of breast cancer to bone in vivo.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 66-97-7
Appearance Solid
Molecular Weight 186.16
Formula C₁₁H₆O₃
Color White to off-white
SMILES

O=C1C=CC2=CC3=C(OC=C3)C=C2O1
Synonyms

Ficusin
Structure Classification
- Phenylpropanoids
- Coumarins
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (6)

All Cell Imaging/StainingCell Proliferation/Viability AssayCell Migration/Invasion AssayWB

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Journal Impact Factor

Anal Chem

Force-Dependent Intercalative Bulky DNA Adduct Formation Detected by Single-Molecule Stretching. [Abstract]

2022 Oct 4;94(39):13623-13630. PMID: 36129494
J Ethnopharmacol

Discovery of bakuchiol as an AIM2 inflammasome activator and cause of hepatotoxicity. [Abstract]

2022 Nov 15:298:115593. PMID: 35973629
Biomedicines

Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells. [Abstract]

2024 Dec 31;13(1):73. PMID: 39857658

Psoralen purchased from MedChemExpress. Usage Cited in: Biomedicines. 2024 Dec 31;13(1):73. [Abstract]

Nuclear staining of 4T1 with Pso (Psoralen) (10 nM; 1 h).

Psoralen purchased from MedChemExpress. Usage Cited in: Biomedicines. 2024 Dec 31;13(1):73. [Abstract]

The combination of Pso (Psoralen) (10 nM; 1 h) and 3 J/cm² UVA had the greatest effect at B16-F10 with a 2.04-fold increase (p < 0.005).
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
In Vivo

Mitochondrial Damage-induced Ferroptosis: The Molecular Mechanism by Which Psoralen Inhibits the Proliferation and Invasion of Non-small-cell Lung Cancer Cells. [Abstract]

2025 Sep-Oct;39(5):2681-2702. PMID: 40877163

Psoralen purchased from MedChemExpress. Usage Cited in: In Vivo. 2025 Sep-Oct;39(5):2681-2702. [Abstract]

Cell viability of A549 cells treated with different concentrations of Psoralen (20-160 μg/mL) for 12, 24, and 48 h, measured using the CCK-8 assay.

Psoralen purchased from MedChemExpress. Usage Cited in: In Vivo. 2025 Sep-Oct;39(5):2681-2702. [Abstract]

Effects of Psoralen (15-60 μg/mL) on A549 cell proliferation, assessed by the 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay.

Psoralen purchased from MedChemExpress. Usage Cited in: In Vivo. 2025 Sep-Oct;39(5):2681-2702. [Abstract]

Wound healing assay assessing the effect of different Psoralen (15-60 μg/mL) concentrations on A549 cell migration. Red lines indicate the wound edges, showing the extent of cell migration at different time points across treatment groups.

Psoralen purchased from MedChemExpress. Usage Cited in: In Vivo. 2025 Sep-Oct;39(5):2681-2702. [Abstract]

Psoralen (15-60 μg/mL; 24 h) increases intracellular Fe²⁺ levels in A549 cells.

Psoralen purchased from MedChemExpress. Usage Cited in: In Vivo. 2025 Sep-Oct;39(5):2681-2702. [Abstract]

Psoralen (15-60 μg/mL; 24 h) disrupts antioxidant defense and enhances lipid peroxidation in A549 cells. Western blot analysis of glutathione peroxidase 4 (GPX4) and solute carrier family 7 member 11 (SLC7A11) protein expression levels in A549 cells treated with psoralen psoralen , with quantitative analysis.
Evid Based Complement Alternat Med

The In Vitro Effect of Psoralen on Glioma Based on Network Pharmacology and Potential Target Research. [Abstract]

2022 Aug 27:2022:1952891. PMID: 36065261

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (537.17 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : < 0.1 mg/mL (insoluble)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.3717 mL

26.8586 mL

53.7172 mL

5 mM

1.0743 mL

5.3717 mL

10.7434 mL

10 mM

0.5372 mL

2.6859 mL

5.3717 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (13.43 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (13.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Li Yin, et al. A novel psoralen derivative-MPFC enhances melanogenesis via activation of p38 MAPK and PKA signaling pathways in B16 cells. Int J Mol Med. 2018 Jun;41(6):3727-3735. [Content Brief]

[2]. Wu C, et al. Psoralen inhibits bone metastasis of breast cancer in mice. Fitoterapia. 2013 Dec;91:205-10. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.3717 mL	26.8586 mL	53.7172 mL	134.2931 mL
	5 mM	1.0743 mL	5.3717 mL	10.7434 mL	26.8586 mL
	10 mM	0.5372 mL	2.6859 mL	5.3717 mL	13.4293 mL
	15 mM	0.3581 mL	1.7906 mL	3.5811 mL	8.9529 mL
	20 mM	0.2686 mL	1.3429 mL	2.6859 mL	6.7147 mL
	25 mM	0.2149 mL	1.0743 mL	2.1487 mL	5.3717 mL
	30 mM	0.1791 mL	0.8953 mL	1.7906 mL	4.4764 mL
	40 mM	0.1343 mL	0.6715 mL	1.3429 mL	3.3573 mL
	50 mM	0.1074 mL	0.5372 mL	1.0743 mL	2.6859 mL
	60 mM	0.0895 mL	0.4476 mL	0.8953 mL	2.2382 mL
	80 mM	0.0671 mL	0.3357 mL	0.6715 mL	1.6787 mL
	100 mM	0.0537 mL	0.2686 mL	0.5372 mL	1.3429 mL

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Psoralen

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Publications Citing Use of MedChemExpress (MCE) Psoralen

All HIV Isoforms

Biological Activity

Chemical Information

Publications (6)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Psoralen Related Classifications

Cat. No.: HY-N0053