Comparison of Radio- and Phototoxicity in Association with an Enhancing Effect of the Photosensitizers Psoralen, Trioxsalen and Ortho-Iodo-Hoechst33258 on FaDu, PC-3, 4T1 and B16-F10 Cells

  • Biomedicines. 2024 Dec 31;13(1):73. doi: 10.3390/biomedicines13010073.
Katja Tietze  1 ,  Florian Brandt  1 ,  Kerstin Wetzig  1 ,  Lisa Hübinger  1 ,  Marc Pretze  1 ,  Ralph Alexander Bundschuh  1 ,  Jörg Kotzerke  1
Affiliations
  • 1. Department of Nuclear Medicine, University Hospital Carl Gustav Carus, Technische Universität Dresden, 01307 Dresden, Germany.
PMID: 39857658 DOI: 10.3390/biomedicines13010073
Abstract

Background: Energy delivered at different wavelengths causes different types of damage to DNA. Methods: PC-3, FaDu, 4T1 and B16-F10 cells were irradiated with different wavelengths of ultraviolet light (UVA/UVC) and ionizing radiation (X-ray). Furthermore, different photosensitizers (ortho-iodo-Hoechst33258/psoralen/trioxsalen) were tested for their amplifying effect. Survival fraction and damage analysis using the γH2A.X assay (double-strand breaks) and the ELISA assay (cyclobutane pyrimidine dimers) were compared. Results: The PC-3 cells were found to be the most sensitive cells to the treatment strategies used. FaDu and PC-3 showed a strong sensitivity to UVA. Analysis of the damage showed that the cell lines exhibited different sensitivities. Conclusions: Thus, an enhancing effect of photosensitizers (PS) in combination with UVA could be demonstrated in some cases. However, this is cell- and dose-dependent.

Keywords
ELISA-assay (cyclobutane pyrimidine dimers); UVA/UVC; X-rays; ortho-iodo-hoechst33258; photosensitizer; psoralen; survival fraction; trioxsalen; γH2A.X-assay (double-strand breaks).
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