Trioxsalen  (Synonyms: Trisoralen; Trioxysalen; TMP)

Trioxsalen (Trisoralen), a psoralen derivative, is a photochemical DNA crosslinker. Trioxsalen only works after photoactivation with near ultraviolet light. Trioxsalen is a photosensitizer that can be used for the research of vitiligo and hand eczema. Trioxsalen is used for visualization of genomic interstrand cross-links localized by laser photoactivation^[1][2][3].

Trioxsalen (200 nM) plus 365 nm light produce DNA crosslinks but not breaks in mouse L1210 leukemia cells^[4].
Trioxsalen/UVA treatment of HaCaT keratinocytes induces NF-kappaB binding activity, and that this is a synergistic effect^[5].
Trioxsalen shows anti-inflammatory capacities in vitro^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Trioxsalen induces dose-dependent depletion of ATPase-positive LCs, with a maximal depletion of 80%, in oral mucous membrane (OMM) with PUVA (UVA doses of 1-8 J/cm2) in rat^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

228.24

C₁₄H₁₂O₃

3902-71-4

Solid

White to off-white

O=C1C=C(C)C2=CC3=C(OC(C)=C3)C(C)=C2O1

Room temperature in continental US; may vary elsewhere.

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

• [1]. M T Chuan, et al. Effectiveness of psoralen photochemotherapy for vitiligo. J Formos Med Assoc. 1999 May;98(5):335-40.  [Content Brief]

  [2]. A Marco van Coevorden, et al. Comparison of oral psoralen-UV-A with a portable tanning unit at home vs hospital-administered bath psoralen-UV-A in patients with chronic hand eczema: an open-label randomized controlled trial of efficacy. Arch Dermatol. 2004 Dec;140(12):1463-6.  [Content Brief]

  [3]. A Kuusilehto, et al. The effect of PUVA on langerhans cells in rat oral epithelium photosensitized with systemic methoxsalen or topical trioxsalen. Photodermatol Photoimmunol Photomed. 2000 Jun;16(3):129-33.  [Content Brief]

  [4]. L F Cohen, et al. Interstrand DNA crosslinking by 4,5'8-trimethylpsoralen plus monochromatic ultraviolet light. Studies by alkaline elution in mouse L1210 leukemia cells. Biochim Biophys Acta. 1980 Nov 14;610(1):56-63.  [Content Brief]

  [5]. H Sánchez Ruderisch, et al. Trioxsalen in the presence of UVA is able to induce nuclear factor kappa B binding activity in HaCaT keratinocytes. Skin Pharmacol Appl Skin Physiol. Sep-Oct 2002;15(5):335-41.  [Content Brief]