1. Anti-infection
    Membrane Transporter/Ion Channel
    Neuronal Signaling
    Metabolic Enzyme/Protease
    Immunology/Inflammation
    NF-κB
    Apoptosis
  2. Parasite
    GABA Receptor
    Reactive Oxygen Species
    Apoptosis
  3. Emamectin Benzoate

Emamectin Benzoate (Synonyms: MK-244)

Cat. No.: HY-B0837 Purity: 99.40%
Handling Instructions

Emamectin Benzoate (MK-244) is an orally active nervoussystem toxicant by binding g-aminobutyric (GABA) receptor in insects. Emamectin Benzoate is one of semi-synthetic derivative of Avermectin (HY-15311) with a broadspectrum of insecticidal and acaricidal activity. Emamectin Benzoate induces ROS-mediated DNA damage and cell apoptosis. Emamectin Benzoate, a mixture of the natural Emamectin B1a benzoate and Emamectin B1b benzoate, has the main component of Emamectin B1a benzoate.

For research use only. We do not sell to patients.

Emamectin Benzoate Chemical Structure

Emamectin Benzoate Chemical Structure

CAS No. : 155569-91-8

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Solution
10 mM * 1 mL in DMSO USD 73 In-stock
Estimated Time of Arrival: December 31
Solid
100 mg USD 66 In-stock
Estimated Time of Arrival: December 31
500 mg USD 119 In-stock
Estimated Time of Arrival: December 31
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Description

Emamectin Benzoate (MK-244) is an orally active nervoussystem toxicant by binding g-aminobutyric (GABA) receptor in insects. Emamectin Benzoate is one of semi-synthetic derivative of Avermectin (HY-15311) with a broadspectrum of insecticidal and acaricidal activity. Emamectin Benzoate induces ROS-mediated DNA damage and cell apoptosis. Emamectin Benzoate, a mixture of the natural Emamectin B1a benzoate and Emamectin B1b benzoate, has the main component of Emamectin B1a benzoate[1][2].

IC50 & Target

GABA Receptor

In Vitro

Emamectin Benzoate (MK-244; 2.5-40 μM; 12 and 24 h) decreases cell viability in a time- and dose-dependent manner[1].
Emamectin Benzoate (2.5-20 μM; 24 hours) induces apoptosis and DNA damage in 16HBE cells. Emamectin Benzoate induces ROS generation in 16HBE cells[1].
Emamectin Benzoate (2.5-20 μM; 12 hours) increases the amounts of cytochrome-c, caspase-3, cas-pase-9, cleaved-PARP, Bax/Bcl-2[1].
Emamectin Benzoate (2.5, 5, 10, 15 μM; 72 h) inhibits cell viability with an IC50 of 3.72 μM in Trichoplusia Tn5B1-4 cell. Emamectin Benzoat induces chromatin condensation in nuclei and cell apoptosis[2].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay[1]

Cell Line: human normal bronchial epithelial cell line 16HBE
Concentration: 2.5, 5, 7.5,10,15, 20, 40 μM
Incubation Time: 12 and 24 hours
Result: Decreased cell viability in a time- and dose-dependent manner wirh IC50s of 11.88 μM and 9.67 μM in 12 and 24 hours, respectively.

Apoptosis Analysis[1]

Cell Line: human normal bronchial epithelial cell line 16HBE
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 24 hours
Result: Induced apoptosis and caused chromatin shrinkage and nuclear fragmentation.

Western Blot Analysis[1]

Cell Line: human normal bronchial epithelial cell line 16HBE
Concentration: 2.5, 5, 10, 20 μM
Incubation Time: 12 hours
Result: Increased the amounts of cytochrome-c, caspase-3, cas-pase-9, cleaved-PARP, Bax/Bcl-2.
In Vivo

Emamectin Benzoate (MK-244; oral; 25-100 mg/kg/day; for 14 days) causes a marked induction of oxidative damage in liver tissue[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: 10 weeks old Swiss albino male mice (25-30 g)[3]
Dosage: 25, 50, 100 mg/kg
Administration: Oral; daily; for 14 days
Result: Caused a marked induction of oxidative damage in liver tissue as demonstrated by an increased level of TBARS and reduced GSH level.
Molecular Weight

1008.24(Based on Emamectin B1a Benzoate)

Formula

C49H75NO13.C7H6O2

CAS No.
SMILES

C[[email protected]]1O[[email protected]@H](O[[email protected]@]2([H])[[email protected]](C)O[[email protected]@H](O[[email protected]]([[email protected]@H](C)/C=C/C=C3CO[[email protected]@]4([H])[[email protected]]\3(O)[[email protected]]5C=C(C)[[email protected]]4O)([H])/C(C)=C/C[[email protected]@H]6C[[email protected]](OC5=O)C[[email protected]]7(C=C[[email protected]](C)[[email protected]@H](C(C)C)O7)O6)C[[email protected]@H]2OC)C[[email protected]@H](OC)[[email protected]@H]1NC.C[[email protected]]8O[[email protected]@H](O[[email protected]@]9([H])[[email protected]](C)O[[email protected]@H](O[[email protected]]([[email protected]@H](C)/C=C/C=C%10CO[[email protected]@]%11([H])[[email protected]]\%10(O)[[email protected]]%12C=C(C)[[email protected]]%11O)([H])/C(C)=C/C[[email protected]@H]%13C[[email protected]](OC%12=O)C[[email protected]]%14(C=C[[email protected]](C)[[email protected]@H]([[email protected]](CC)C)O%14)O%13)C[[email protected]@H]9OC)C[[email protected]@H](OC)[[email protected]@H]8NC.O=C(O)C%15=CC=CC=C%15

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility
In Vitro: 

DMSO : ≥ 31 mg/mL

*"≥" means soluble, but saturation unknown.

In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.08 mg/mL (Infinity mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% (20% SBE-β-CD in saline)

    Solubility: ≥ 2.08 mg/mL (Infinity mM); Clear solution

  • 3.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (Infinity mM); Clear solution

*All of the co-solvents are available by MCE.
References

Purity: 99.40%

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Emamectin Benzoate
Cat. No.:
HY-B0837
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